Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VXJ35_RS22500 Genome accession   NZ_CP143089
Coordinates   4922228..4923361 (-) Length   377 a.a.
NCBI ID   WP_064023282.1    Uniprot ID   A0A172UEK3
Organism   Methylomonas sp. DH-1 strain CS40     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4917228..4928361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXJ35_RS22475 - 4917784..4919031 (+) 1248 WP_064023653.1 IS256 family transposase -
  VXJ35_RS22480 - 4919016..4919354 (-) 339 WP_064023280.1 hypothetical protein -
  VXJ35_RS22485 - 4919367..4919537 (-) 171 WP_157198267.1 hypothetical protein -
  VXJ35_RS22490 - 4919595..4920188 (-) 594 WP_064023281.1 recombinase family protein -
  VXJ35_RS22495 mltB 4921032..4922210 (-) 1179 WP_231914812.1 lytic murein transglycosylase B -
  VXJ35_RS22500 pilU 4922228..4923361 (-) 1134 WP_064023282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VXJ35_RS22505 pilT 4923378..4924415 (-) 1038 WP_054759731.1 type IV pilus twitching motility protein PilT Machinery gene
  VXJ35_RS22510 - 4924591..4925613 (-) 1023 WP_064023283.1 SPFH domain-containing protein -
  VXJ35_RS22515 - 4925813..4926436 (+) 624 WP_064023284.1 TPM domain-containing protein -
  VXJ35_RS22520 - 4926433..4927941 (+) 1509 WP_064023285.1 TIM44-like domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42058.29 Da        Isoelectric Point: 6.6668

>NTDB_id=928222 VXJ35_RS22500 WP_064023282.1 4922228..4923361(-) (pilU) [Methylomonas sp. DH-1 strain CS40]
MDFTALLALMVEKRASDLFITAGRPPSMKVNGKVVEVSKTPLTSDQTMKLVLGIMSQRQRDEFENTKECQFALSAHSLGR
FRVSAFTQRDSAGMVLRRIETTIPTAEELHLPPVLKELIMHKRGLVMFVGATGTGKSTSLAALIRHRNENSSGHIISIED
PIEFIHPHQGCIITQREVGLDTESFEVALKNTLRQAPDVILIGEVRTRETMQHAITFAETGHLCVCTLHANNANQALDRI
LHFFPEDMHSQLFMDLSLNLRGIVAQQLIQRADGKGRYPAIEILLNTPLVADLVRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYTAGKISYEDALHAADSRNEVRLMIKLGAENVNFETAGMSLAENDEDGGSLYK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=928222 VXJ35_RS22500 WP_064023282.1 4922228..4923361(-) (pilU) [Methylomonas sp. DH-1 strain CS40]
ATGGACTTTACCGCGCTATTGGCATTGATGGTGGAAAAACGGGCTTCGGATTTATTCATCACCGCCGGGCGGCCGCCGTC
GATGAAAGTCAACGGCAAAGTCGTCGAAGTCTCGAAGACGCCGCTGACCAGCGACCAAACCATGAAACTGGTACTCGGCA
TCATGTCGCAGCGCCAGCGCGACGAATTCGAAAACACCAAGGAATGCCAATTTGCATTAAGCGCGCACAGCCTGGGCCGA
TTCCGGGTCAGCGCCTTTACCCAGCGCGATTCCGCCGGCATGGTGTTGCGCCGGATCGAAACCACGATTCCGACCGCGGA
AGAATTGCATCTGCCGCCGGTGTTGAAAGAATTGATCATGCACAAGCGCGGCCTGGTGATGTTCGTCGGCGCGACCGGTA
CCGGTAAATCGACCTCGTTGGCGGCCTTGATTCGCCACCGCAACGAAAACAGCAGCGGCCACATCATTTCCATCGAAGAT
CCGATCGAGTTCATCCATCCGCACCAGGGCTGCATCATTACTCAGCGCGAAGTGGGGCTGGATACCGAATCGTTCGAAGT
GGCTTTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATTCTGATCGGCGAGGTTCGCACCCGCGAGACGATGCAACACG
CGATTACCTTTGCCGAGACCGGCCATCTCTGCGTCTGCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
TTACATTTCTTCCCCGAAGACATGCACAGCCAGTTGTTCATGGATTTGTCGCTGAATCTGCGCGGGATCGTCGCCCAACA
ATTGATCCAACGCGCCGACGGCAAGGGCCGCTATCCGGCCATCGAAATTCTGTTGAATACGCCGTTGGTCGCCGACCTGG
TGCGCAAAGGCGAAGTGCATAAATTGAAGGAGCTGATGAAAAACTCGCGCGAGCATGGCATGCAGACTTTCGACCAGGCG
CTTTACGATCTGTACACAGCCGGTAAAATTAGTTACGAAGATGCTCTACACGCTGCCGACTCCCGCAACGAAGTCCGCCT
GATGATCAAACTGGGGGCCGAGAACGTCAATTTCGAAACCGCCGGCATGTCGCTGGCCGAGAACGACGAAGATGGAGGCA
GCCTTTATAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172UEK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.241

100

0.69
  pilU Acinetobacter baylyi ADP1

64.225

94.164

0.605
  pilU Vibrio cholerae strain A1552

57.429

92.838

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.253

92.308

0.408
  pilT Legionella pneumophila strain ERS1305867

41.888

89.92

0.377
  pilT Legionella pneumophila strain Lp02

41.888

89.92

0.377
  pilT Pseudomonas aeruginosa PAK

41.003

89.92

0.369
  pilT Acinetobacter nosocomialis M2

40.413

89.92

0.363
  pilT Acinetobacter baumannii D1279779

40.413

89.92

0.363
  pilT Acinetobacter baumannii strain A118

40.413

89.92

0.363
  pilT Acinetobacter baylyi ADP1

39.883

90.451

0.361