Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VOI35_RS07840 Genome accession   NZ_CP142685
Coordinates   1685362..1686411 (+) Length   349 a.a.
NCBI ID   WP_407275506.1    Uniprot ID   -
Organism   Halothiobacillus sp. DCM-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1680362..1691411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VOI35_RS07815 (VOI35_07805) metW 1681284..1681883 (-) 600 WP_407275501.1 methionine biosynthesis protein MetW -
  VOI35_RS07820 (VOI35_07810) - 1681880..1683037 (-) 1158 WP_407275502.1 homoserine O-acetyltransferase -
  VOI35_RS07825 (VOI35_07815) - 1683064..1683618 (-) 555 WP_407275503.1 YggT family protein -
  VOI35_RS07830 (VOI35_07820) proC 1683615..1684457 (-) 843 WP_407275504.1 pyrroline-5-carboxylate reductase -
  VOI35_RS07835 (VOI35_07825) - 1684523..1685233 (-) 711 WP_407275505.1 YggS family pyridoxal phosphate-dependent enzyme -
  VOI35_RS07840 (VOI35_07830) pilT 1685362..1686411 (+) 1050 WP_407275506.1 type IV pilus twitching motility protein PilT Machinery gene
  VOI35_RS07845 (VOI35_07835) pilU 1686480..1687628 (+) 1149 WP_407275507.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VOI35_RS07850 (VOI35_07840) - 1687671..1688834 (+) 1164 WP_407275508.1 PilT/PilU family type 4a pilus ATPase -
  VOI35_RS07855 (VOI35_07845) - 1689181..1689519 (+) 339 WP_407275509.1 c-type cytochrome -
  VOI35_RS07860 (VOI35_07850) - 1689528..1690895 (+) 1368 WP_407275510.1 FCSD flavin-binding domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38928.58 Da        Isoelectric Point: 7.3028

>NTDB_id=926415 VOI35_RS07840 WP_407275506.1 1685362..1686411(+) (pilT) [Halothiobacillus sp. DCM-1]
MAEQRGIDTLLAFTVKNGASDLHLSANEPPRVRVDGDIRRINFPVMEHKEVHAMVYDIMNDKQRKEYEDNLEVDFSFELP
GLARFRVNAYNQNRGAAAVFRTIPSEVLSLEQLAAPNVFKELCMFPRGIVLVTGPTGSGKSTTLAAMINHINDNRNDHIL
TIEDPIEFVHQSKKCLINQREVHRDTHSFNAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKT
IDRVVDVFPAAEKDMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGQTQGMQTL
DQNLLQLMKRGIISREDARARAQNKADFA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=926415 VOI35_RS07840 WP_407275506.1 1685362..1686411(+) (pilT) [Halothiobacillus sp. DCM-1]
ATGGCAGAACAACGCGGCATTGACACCTTACTCGCCTTCACCGTCAAGAACGGCGCTTCGGATCTTCACCTTTCCGCCAA
CGAACCGCCTCGCGTGCGGGTCGATGGGGATATTCGCCGGATCAATTTCCCGGTGATGGAGCATAAAGAAGTGCACGCCA
TGGTGTATGACATCATGAACGACAAACAGCGGAAGGAATATGAAGACAACCTCGAAGTGGATTTTTCCTTCGAGCTCCCC
GGCCTCGCCCGCTTCCGGGTGAATGCGTATAACCAGAACCGGGGCGCGGCCGCCGTTTTCCGTACGATTCCGAGCGAAGT
GCTCTCCTTAGAGCAGCTCGCCGCACCCAATGTGTTCAAAGAGCTGTGCATGTTCCCGCGCGGCATCGTGCTGGTGACCG
GCCCGACCGGCTCGGGCAAGTCCACCACCCTGGCCGCGATGATCAACCACATCAATGACAATCGGAATGACCACATTCTG
ACCATTGAAGACCCGATCGAATTCGTCCATCAGAGCAAAAAATGCCTGATCAACCAACGCGAAGTCCATCGCGACACCCA
CAGCTTCAACGCTGCGCTGCGCTCCGCTCTGCGGGAGGATCCGGATGTCATCCTGGTCGGCGAATTGCGCGATCTTGAAA
CCATTCGCCTGGCGCTGACCGCCGCAGAAACTGGCCACCTAGTGTTCGCCACCCTGCACACCAGTTCGGCGGCCAAGACG
ATCGACCGGGTCGTCGATGTCTTCCCCGCGGCCGAAAAAGACATGGTTCGCTCGATGCTCTCGGAATCCCTGCGCGCCGT
CATCTCGCAAACCCTGCTCAAGAAGATCGGCGGCGGGCGGGTGGCCGCGCACGAAATCATGCTGGGCACCCCCGCCATTC
GCAACCTGATCCGCGAAAACAAGATCGCCCAGATGTATTCCGCCATCCAAACCGGCCAGACACAGGGCATGCAAACCCTG
GATCAGAACCTGTTGCAGTTGATGAAGCGCGGGATTATTTCCCGTGAAGATGCACGGGCACGGGCGCAAAACAAAGCCGA
TTTCGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.193

97.994

0.756
  pilT Acinetobacter baumannii D1279779

77.193

97.994

0.756
  pilT Acinetobacter baumannii strain A118

77.193

97.994

0.756
  pilT Pseudomonas aeruginosa PAK

75.146

97.994

0.736
  pilT Acinetobacter baylyi ADP1

74.561

97.994

0.731
  pilT Pseudomonas stutzeri DSM 10701

73.977

97.994

0.725
  pilT Legionella pneumophila strain Lp02

72.807

97.994

0.713
  pilT Legionella pneumophila strain ERS1305867

72.807

97.994

0.713
  pilT Neisseria meningitidis 8013

69.767

98.567

0.688
  pilT Neisseria gonorrhoeae MS11

69.477

98.567

0.685
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

96.562

0.665
  pilT Vibrio cholerae strain A1552

68.843

96.562

0.665
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.28

99.427

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.399

98.281

0.407
  pilU Acinetobacter baylyi ADP1

40.299

95.989

0.387
  pilU Vibrio cholerae strain A1552

40.785

94.842

0.387