Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VOI35_RS07845 Genome accession   NZ_CP142685
Coordinates   1686480..1687628 (+) Length   382 a.a.
NCBI ID   WP_407275507.1    Uniprot ID   -
Organism   Halothiobacillus sp. DCM-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1681480..1692628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VOI35_RS07820 (VOI35_07810) - 1681880..1683037 (-) 1158 WP_407275502.1 homoserine O-acetyltransferase -
  VOI35_RS07825 (VOI35_07815) - 1683064..1683618 (-) 555 WP_407275503.1 YggT family protein -
  VOI35_RS07830 (VOI35_07820) proC 1683615..1684457 (-) 843 WP_407275504.1 pyrroline-5-carboxylate reductase -
  VOI35_RS07835 (VOI35_07825) - 1684523..1685233 (-) 711 WP_407275505.1 YggS family pyridoxal phosphate-dependent enzyme -
  VOI35_RS07840 (VOI35_07830) pilT 1685362..1686411 (+) 1050 WP_407275506.1 type IV pilus twitching motility protein PilT Machinery gene
  VOI35_RS07845 (VOI35_07835) pilU 1686480..1687628 (+) 1149 WP_407275507.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VOI35_RS07850 (VOI35_07840) - 1687671..1688834 (+) 1164 WP_407275508.1 PilT/PilU family type 4a pilus ATPase -
  VOI35_RS07855 (VOI35_07845) - 1689181..1689519 (+) 339 WP_407275509.1 c-type cytochrome -
  VOI35_RS07860 (VOI35_07850) - 1689528..1690895 (+) 1368 WP_407275510.1 FCSD flavin-binding domain-containing protein -
  VOI35_RS07865 (VOI35_07855) - 1691024..1692481 (+) 1458 WP_407275511.1 cytochrome c -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42846.03 Da        Isoelectric Point: 6.1931

>NTDB_id=926416 VOI35_RS07845 WP_407275507.1 1686480..1687628(+) (pilU) [Halothiobacillus sp. DCM-1]
MDRQQAANYVQGLLRKAVDLNASDLFVTVGAPPSAKINGEITQLTEQALSTNHTQMLVRSIMNDKQVAAFEETREANFAI
SLPGVARFRVNAFMQRGSVGMVLRMIRSDVPKFDELLLPKVLEDIAMTKRGLVIFVGATSSGKSTSLAAMLDYRNENSAG
HIVTIEDPIEYVHRHKKCLVTQREVGVDTETFESALKNTLRQAPDVILIGEIRERETMDHAIAFAETGHLCLATLHANNT
NQALDRIINFFPEERRHQLLMDLSLNLKAVVSQRLLRKEEGSGRVPAVEVLINTPLIGDLIFKGDVHEIKEVMKRSREHG
MQTFDQALLDLYDQGLVSYQEALRNADSQNDLRLSIKLNSKRGLPKNMMETDDGLRIEKDDE

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=926416 VOI35_RS07845 WP_407275507.1 1686480..1687628(+) (pilU) [Halothiobacillus sp. DCM-1]
ATGGATCGTCAGCAAGCCGCCAACTATGTTCAAGGCCTTCTGCGCAAGGCCGTAGACCTGAATGCCTCGGATTTATTTGT
CACCGTCGGCGCGCCCCCTTCCGCCAAAATCAATGGCGAAATCACCCAGCTCACTGAGCAGGCACTCTCGACTAACCATA
CCCAGATGCTGGTTCGGTCGATCATGAATGACAAACAGGTGGCCGCATTTGAAGAAACCCGTGAGGCCAACTTCGCCATC
TCCCTGCCCGGCGTGGCGCGTTTTCGCGTGAATGCCTTCATGCAGCGCGGCAGCGTCGGCATGGTGTTGCGGATGATTCG
TTCGGATGTGCCCAAATTCGACGAGCTGCTCTTGCCCAAAGTGCTCGAAGATATCGCCATGACCAAGCGCGGTCTGGTGA
TTTTCGTCGGCGCGACCAGCTCAGGAAAATCCACCTCACTGGCGGCAATGCTCGATTACCGCAATGAAAACTCTGCCGGG
CACATCGTCACCATCGAAGACCCGATCGAGTATGTGCATCGCCATAAAAAGTGCCTGGTCACGCAGCGTGAAGTCGGCGT
GGATACGGAAACCTTCGAGTCCGCGCTGAAAAACACCCTGCGGCAAGCACCCGATGTCATCCTGATTGGCGAGATTCGTG
AACGCGAAACCATGGATCACGCCATTGCCTTCGCCGAAACCGGCCACTTGTGCCTTGCGACCCTGCACGCCAACAACACC
AACCAGGCACTCGACCGGATCATCAACTTTTTCCCCGAAGAACGGCGGCATCAGCTGTTGATGGATCTCTCGCTCAACCT
CAAGGCGGTCGTGTCGCAGCGGCTGCTCCGCAAGGAAGAAGGCAGTGGGCGCGTGCCGGCGGTCGAAGTGCTGATCAACA
CCCCGTTGATCGGCGATTTGATTTTTAAGGGCGATGTACACGAAATCAAAGAGGTTATGAAACGCTCGCGTGAGCACGGC
ATGCAAACCTTCGACCAAGCCCTGTTGGATCTATACGATCAGGGGCTGGTCAGCTATCAGGAAGCCCTTCGTAATGCTGA
TTCGCAAAATGATCTGCGCCTGTCGATCAAACTCAACAGTAAACGCGGCCTGCCGAAAAACATGATGGAAACCGATGATG
GTTTGCGGATCGAGAAAGACGACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.811

96.859

0.589
  pilU Acinetobacter baylyi ADP1

59.42

90.314

0.537
  pilU Vibrio cholerae strain A1552

56.901

92.932

0.529
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.349

90.052

0.408
  pilT Acinetobacter baumannii strain A118

45.697

88.22

0.403
  pilT Acinetobacter nosocomialis M2

45.697

88.22

0.403
  pilT Acinetobacter baumannii D1279779

45.697

88.22

0.403
  pilT Acinetobacter baylyi ADP1

44.94

87.958

0.395
  pilT Pseudomonas stutzeri DSM 10701

44.611

87.435

0.39
  pilT Pseudomonas aeruginosa PAK

44.012

87.435

0.385
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.465

86.126

0.374
  pilT Vibrio cholerae strain A1552

43.465

86.126

0.374
  pilT Neisseria meningitidis 8013

41.867

86.911

0.364