Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OPV09_RS05555 Genome accession   NZ_CP142523
Coordinates   1226830..1227873 (-) Length   347 a.a.
NCBI ID   WP_034749074.1    Uniprot ID   -
Organism   Janthinobacterium sp. TB1-E2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1221830..1232873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPV09_RS05540 (OPV09_05540) - 1222907..1225015 (-) 2109 WP_338680824.1 EAL domain-containing protein -
  OPV09_RS05545 (OPV09_05545) - 1225200..1225685 (-) 486 WP_219329983.1 glutathione peroxidase -
  OPV09_RS05550 (OPV09_05550) pilU 1225687..1226826 (-) 1140 WP_338680825.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OPV09_RS05555 (OPV09_05555) pilT 1226830..1227873 (-) 1044 WP_034749074.1 type IV pilus twitching motility protein PilT Machinery gene
  OPV09_RS05560 (OPV09_05560) - 1227944..1228645 (+) 702 WP_338680826.1 YggS family pyridoxal phosphate-dependent enzyme -
  OPV09_RS05565 (OPV09_05565) proC 1228662..1229492 (+) 831 WP_338680827.1 pyrroline-5-carboxylate reductase -
  OPV09_RS05570 (OPV09_05570) - 1229496..1230212 (-) 717 WP_338680828.1 anti-sigma factor -
  OPV09_RS05575 (OPV09_05575) - 1230209..1230784 (-) 576 WP_230518294.1 sigma-70 family RNA polymerase sigma factor -
  OPV09_RS05580 (OPV09_05580) - 1230859..1231350 (-) 492 WP_139143513.1 DUF3455 domain-containing protein -
  OPV09_RS05585 (OPV09_05585) - 1231507..1231854 (-) 348 WP_070305746.1 hypothetical protein -
  OPV09_RS05590 (OPV09_05590) - 1231851..1232228 (-) 378 WP_081368364.1 phage holin family protein -
  OPV09_RS05595 (OPV09_05595) - 1232294..1232650 (-) 357 WP_046683402.1 DUF883 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38085.57 Da        Isoelectric Point: 6.6378

>NTDB_id=926024 OPV09_RS05555 WP_034749074.1 1226830..1227873(-) (pilT) [Janthinobacterium sp. TB1-E2]
MDISELLAFSVSNKASDLHLSSGLPPMIRVNGDVRRLNVPPLEHKEVHSMIYDIMNDSQRKAYEEALECDFSFEIPGLAR
FRVNAYNQERGASAVLRTIPSKVLTLEDLNAPRIFGELAMRPRGLVLVTGPTGSGKSTTLAAMVNHVNERLNHHILTIED
PIEFVHEPKKCLINQREVGSHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKSIDRI
IDVFPAEEKEMVRAMLSESLQAVISQNLLKTRDGAGRVAAHEIMLATPAVRNLIREAKVAQMYSAIQTGSNVGMQTLDQC
LSDLVRRGTISAETARSAAKAPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=926024 OPV09_RS05555 WP_034749074.1 1226830..1227873(-) (pilT) [Janthinobacterium sp. TB1-E2]
ATGGACATCTCCGAACTACTCGCTTTCTCCGTCAGCAACAAGGCTTCCGACTTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATACGGGTCAACGGCGACGTGCGCCGCCTGAACGTGCCCCCGCTCGAGCACAAGGAAGTGCACAGCATGATCTACGACA
TCATGAACGACAGCCAGCGCAAGGCGTATGAAGAAGCGCTGGAATGCGATTTCTCGTTCGAGATTCCCGGCCTGGCCCGC
TTCCGCGTCAACGCCTACAACCAGGAAAGGGGCGCCTCGGCCGTGCTGCGCACGATTCCGTCAAAGGTGCTGACACTGGA
AGACCTGAACGCGCCGCGCATCTTCGGCGAACTGGCCATGCGCCCACGCGGCCTCGTGTTGGTGACGGGGCCGACCGGTT
CCGGCAAGTCGACCACCCTGGCGGCGATGGTGAACCACGTCAACGAACGCCTGAACCACCATATTTTGACCATCGAAGAC
CCGATCGAATTCGTGCATGAACCGAAGAAATGCCTGATCAACCAGCGCGAAGTGGGTTCGCACACGCATTCGTTCAGCAA
CGCGCTGCGCTCGGCCCTGCGCGAAGACCCGGACGTGATTTTAGTGGGCGAATTGCGCGACCTGGAAACCATCCGTCTGG
CGCTGACGGCGGCCGAAACGGGCCACCTCGTGTTCGGCACCCTGCACACCTCGTCGGCGGCGAAATCGATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCCCTGCAAGCCGTCATTTCGCAAAA
CCTGCTGAAAACCAGGGATGGCGCGGGCCGCGTGGCCGCGCATGAAATCATGCTGGCCACGCCGGCCGTGCGCAACCTGA
TCCGCGAAGCGAAAGTGGCGCAAATGTATTCAGCCATCCAGACAGGCAGCAACGTGGGTATGCAGACGCTGGACCAGTGC
CTGTCCGACCTGGTGCGACGCGGCACGATCTCGGCCGAGACGGCCCGCTCGGCCGCCAAGGCCCCTGAAAACTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.623

99.424

0.732
  pilT Legionella pneumophila strain Lp02

71.976

97.695

0.703
  pilT Legionella pneumophila strain ERS1305867

71.976

97.695

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.118

0.689
  pilT Vibrio cholerae strain A1552

70.92

97.118

0.689
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Vibrio cholerae strain A1552

39.71

99.424

0.395