Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU50_RS12180 Genome accession   NZ_AP025514
Coordinates   2712336..2713373 (-) Length   345 a.a.
NCBI ID   WP_060468650.1    Uniprot ID   A0A109D7I3
Organism   Vibrio toranzoniae strain CECT 7225     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2707336..2718373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU50_RS12155 rsmE 2708046..2708777 (+) 732 WP_017056527.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU50_RS12160 gshB 2708787..2709737 (+) 951 WP_060468647.1 glutathione synthase -
  OCU50_RS12165 - 2709844..2710407 (+) 564 WP_060468648.1 YqgE/AlgH family protein -
  OCU50_RS12170 ruvX 2710480..2710902 (+) 423 WP_029626997.1 Holliday junction resolvase RuvX -
  OCU50_RS12175 pilU 2711211..2712320 (-) 1110 WP_060468649.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU50_RS12180 pilT 2712336..2713373 (-) 1038 WP_060468650.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU50_RS12185 - 2713400..2714104 (+) 705 WP_060468651.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU50_RS12190 proC 2714189..2715007 (+) 819 WP_060468652.1 pyrroline-5-carboxylate reductase -
  OCU50_RS12195 - 2715084..2715641 (+) 558 WP_060468653.1 YggT family protein -
  OCU50_RS12200 yggU 2715671..2715961 (+) 291 WP_060468654.1 DUF167 family protein YggU -
  OCU50_RS12205 - 2715990..2716421 (+) 432 WP_060468655.1 DUF4426 domain-containing protein -
  OCU50_RS12210 - 2716469..2717068 (+) 600 WP_060468656.1 XTP/dITP diphosphatase -
  OCU50_RS12215 hemW 2717068..2718255 (+) 1188 WP_060468657.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38167.68 Da        Isoelectric Point: 6.4426

>NTDB_id=92417 OCU50_RS12180 WP_060468650.1 2712336..2713373(-) (pilT) [Vibrio toranzoniae strain CECT 7225]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPALSHADVHRLVFEIMNDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRSIPVEIPTLGQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSRF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=92417 OCU50_RS12180 WP_060468650.1 2712336..2713373(-) (pilT) [Vibrio toranzoniae strain CECT 7225]
ATGGATATCACCGAGTTACTAGATTTTAGTGTAAAGCATAACGCATCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGAAAGCTTGGAGTGCCCGCTTTGAGTCATGCTGATGTGCATCGTTTAGTTTTTGAGA
TCATGAACGATTCACAGCGCGGTGAGTTTGAAGAGAAACTCGAAGTCGACTTCTCTTTTGAATTGCCCAATGTTGGTCGT
TTCCGTGTGAATGCTTTTAACCAAGCTCGTGGTTGCTCAGCTGTGTTTCGATCTATCCCTGTAGAGATCCCAACTTTAGG
TCAGTTAGGCGCGCCTGAAATCTTTGAAAAGATTGCTAACTACGAGAAAGGGTTAGTGCTGGTCACTGGCCCTACTGGTT
CTGGTAAATCGACGACGCTTGCGGCGATGGTTGATTATGTTAACCGTAACCATAACAAGCACATCCTGACCATTGAAGAC
CCGATTGAATTCGTTCATACCAACAATAAATGCCTCGTTAACCAGCGAGAAGTTCATCGTGATACCCACAGCTTTAAAGC
GGCGCTACGCAGTGCATTGCGCGAAGACCCTGACGTTATCCTTGTGGGTGAACTTCGTGACCAAGAGACAATCAGCTTAG
CGCTAACGGCCGCAGAAACAGGCCATCTAGTTTTTGGTACGCTGCACACCAGTTCTGCTGCTAAAACCATAGATCGTATT
ATTGATGTGTTCCCCGGCAGCGACAAATCTATGGTTCGTTCAATGCTATCCGAATCACTACGCTCGGTGATTGCTCAAAA
ATTGTTAAAGCGTGTAGGCGGTGGTCGTGTGGCTTGTCATGAAATTATGATGGCGACACCTGCAATTAGAAACTTGATTC
GTGAAGATAAAGTTGCACAGATGTACTCGATTATTCAAACTGGCGCAGCACATGGTATGCAAACCATGGAGCAGAATGCG
AAGCAGCTTATGGCCCAAGGTTTAGTTGATTCAGAAGAAGTCGAGAAGAAGATTGAAATCGAAACCTCACGGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A109D7I3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

71.687

96.232

0.69
  pilT Acinetobacter baumannii strain A118

71.687

96.232

0.69
  pilT Acinetobacter nosocomialis M2

71.386

96.232

0.687
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Acinetobacter baylyi ADP1

70.606

95.652

0.675
  pilT Legionella pneumophila strain Lp02

68.085

95.362

0.649
  pilT Legionella pneumophila strain ERS1305867

68.085

95.362

0.649
  pilT Neisseria meningitidis 8013

62.941

98.551

0.62
  pilT Neisseria gonorrhoeae MS11

62.647

98.551

0.617
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.44

98.841

0.499
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.812

0.397
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment