Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU50_RS12175 Genome accession   NZ_AP025514
Coordinates   2711211..2712320 (-) Length   369 a.a.
NCBI ID   WP_060468649.1    Uniprot ID   A0A109D7J0
Organism   Vibrio toranzoniae strain CECT 7225     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2706211..2717320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU50_RS12145 - 2706572..2707072 (+) 501 WP_060468645.1 SprT family zinc-dependent metalloprotease -
  OCU50_RS12150 - 2707169..2707900 (+) 732 WP_060468646.1 endonuclease -
  OCU50_RS12155 rsmE 2708046..2708777 (+) 732 WP_017056527.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU50_RS12160 gshB 2708787..2709737 (+) 951 WP_060468647.1 glutathione synthase -
  OCU50_RS12165 - 2709844..2710407 (+) 564 WP_060468648.1 YqgE/AlgH family protein -
  OCU50_RS12170 ruvX 2710480..2710902 (+) 423 WP_029626997.1 Holliday junction resolvase RuvX -
  OCU50_RS12175 pilU 2711211..2712320 (-) 1110 WP_060468649.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU50_RS12180 pilT 2712336..2713373 (-) 1038 WP_060468650.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU50_RS12185 - 2713400..2714104 (+) 705 WP_060468651.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU50_RS12190 proC 2714189..2715007 (+) 819 WP_060468652.1 pyrroline-5-carboxylate reductase -
  OCU50_RS12195 - 2715084..2715641 (+) 558 WP_060468653.1 YggT family protein -
  OCU50_RS12200 yggU 2715671..2715961 (+) 291 WP_060468654.1 DUF167 family protein YggU -
  OCU50_RS12205 - 2715990..2716421 (+) 432 WP_060468655.1 DUF4426 domain-containing protein -
  OCU50_RS12210 - 2716469..2717068 (+) 600 WP_060468656.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41349.20 Da        Isoelectric Point: 6.0667

>NTDB_id=92416 OCU50_RS12175 WP_060468649.1 2711211..2712320(-) (pilU) [Vibrio toranzoniae strain CECT 7225]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEKLNTAQVSQLLDAMMEQDRRDEYQQTREANFAIVRDSGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMATMTGYRNANRSGHILTIEDPI
EFVHEHRKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNANGRHGVFEVLLNSPRVSDLIRRGELHELKSTMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTKRGDGDNGMGALSSVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92416 OCU50_RS12175 WP_060468649.1 2711211..2712320(-) (pilU) [Vibrio toranzoniae strain CECT 7225]
ATGGAGTTGAATCAAATTCTTGAAGGCATGCTTTCACAGAAAGCGTCGGATCTTTACATCACCGTGGACGCACCAGTTCT
GTTTCGTGTTGATGGTGAACTGCGACCTCAGGGAGAAAAGCTGAATACGGCTCAGGTCTCTCAATTGCTTGATGCGATGA
TGGAGCAAGACCGACGCGATGAATACCAACAAACACGCGAGGCTAATTTTGCGATTGTGCGTGATAGTGGCCGTTTTCGT
GTGAGTGCTTTCTTCCAACGAGAGCTTCCTGGTGCGGTAATTAGACGCATCGAGACTAACATTCCAACCTTTGAACAATT
AAAACTCCCTGATGTGCTACAAGACCTTTCAATAGCGAAACGCGGACTTGTGCTAGTGGTTGGAGCGACGGGGTCTGGTA
AATCAACTTCAATGGCTACGATGACAGGGTACCGGAATGCTAACCGGTCTGGTCATATTTTGACGATTGAAGATCCGATT
GAATTCGTACATGAGCATAGAAAGTGCATTGTTACTCAGCGTGAGGTCGGACTCGACACCGAGAGTTATGAAGTCGCGCT
TAAGAACTCCCTGCGCCAAGCTCCCGACATGATCTTGATTGGTGAGATCCGTAGCCGTGAAACCATGGAGTACGCGATGA
CTTTTGCTGAGACTGGTCACCTATGTATGGCAACTCTGCATGCTAATAATGCTAATCAAGCACTAGAACGTATTCTGCAT
TTGGTTCCCAAAGAGCAAAAAGAGCAATTCTTGTTTGATCTGTCGATGAACTTACGTGGTGTGGTGGCACAGCAATTAAT
TCGAGACAAGAATGCCAATGGTCGTCATGGGGTGTTCGAGGTTCTTTTGAACAGCCCTCGAGTGTCGGACCTGATTCGTC
GTGGTGAGTTGCATGAACTCAAATCGACAATGGCTAAATCGAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GATTTAGTCGTTGCGGGCAAAATCAGCGAAGAAGATGCGTTTCATAGTGCCGACTCTGCTAATGATCTGCGCTTAATGTT
GAAAACCAAGCGAGGTGATGGTGACAATGGCATGGGAGCTCTCAGCAGTGTGAAAATCGATATGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A109D7J0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.424

99.729

0.832
  pilU Pseudomonas stutzeri DSM 10701

58.571

94.851

0.556
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain ERS1305867

41.298

91.87

0.379
  pilT Legionella pneumophila strain Lp02

41.298

91.87

0.379
  pilT Pseudomonas aeruginosa PAK

41.194

90.786

0.374
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.284

87.805

0.371
  pilT Acinetobacter nosocomialis M2

40.118

91.87

0.369
  pilT Acinetobacter baumannii D1279779

40.118

91.87

0.369
  pilT Acinetobacter baumannii strain A118

40.118

91.87

0.369
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.701

90.786

0.36

Multiple sequence alignment