Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU90_RS14925 Genome accession   NZ_AP025508
Coordinates   3369148..3370185 (-) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio splendidus strain LMG 19031     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3364148..3375185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU90_RS14895 rsmE 3364829..3365560 (+) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU90_RS14900 gshB 3365570..3366520 (+) 951 WP_017077777.1 glutathione synthase -
  OCU90_RS14905 - 3366637..3367200 (+) 564 WP_017095267.1 YqgE/AlgH family protein -
  OCU90_RS14910 ruvX 3367274..3367696 (+) 423 WP_017084336.1 Holliday junction resolvase RuvX -
  OCU90_RS14915 - 3367796..3367948 (+) 153 WP_017084337.1 hypothetical protein -
  OCU90_RS14920 pilU 3368024..3369133 (-) 1110 WP_004735479.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU90_RS14925 pilT 3369148..3370185 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU90_RS14930 - 3370212..3370916 (+) 705 WP_061025919.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU90_RS14935 proC 3371029..3371847 (+) 819 WP_017077774.1 pyrroline-5-carboxylate reductase -
  OCU90_RS14940 - 3371923..3372480 (+) 558 WP_004735475.1 YggT family protein -
  OCU90_RS14945 yggU 3372510..3372800 (+) 291 WP_054541895.1 DUF167 family protein YggU -
  OCU90_RS14950 - 3372829..3373260 (+) 432 WP_004735472.1 DUF4426 domain-containing protein -
  OCU90_RS14955 - 3373309..3373908 (+) 600 WP_061025922.1 XTP/dITP diphosphatase -
  OCU90_RS14960 hemW 3373908..3375095 (+) 1188 WP_054541896.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=92323 OCU90_RS14925 WP_004735478.1 3369148..3370185(-) (pilT) [Vibrio splendidus strain LMG 19031]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=92323 OCU90_RS14925 WP_004735478.1 3369148..3370185(-) (pilT) [Vibrio splendidus strain LMG 19031]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGCCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAATTTGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGT
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCTGTGTTCCGTACCATTCCAGTAGAGATTCCCACCCTTGA
ACAGCTAGGGGCTCCTGAGATCTTTGAAAGAATATCTAATTACGAAAAAGGGCTAGTATTAGTCACTGGGCCGACTGGTT
CAGGTAAGTCGACAACTTTGGCAGCGATGGTGGATTACGTTAACCGTAACCATAATAAGCATATATTGACGATTGAAGAC
CCGATCGAATTTGTTCATACCAACAACAAATGCCTAATCAACCAACGAGAAGTGCATCGCGATACTCACAGCTTCAAAGC
GGCATTGCGTAGTGCATTGCGTGAAGATCCAGACGTAATCCTTGTTGGCGAACTTCGTGACCAAGAGACGATTAGCTTAG
CGCTAACCGCAGCAGAAACTGGCCACTTAGTTTTTGGTACCTTGCATACTAGCTCTGCGGCAAAAACCATAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTAAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATCAGAAACTTGATCC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGGGCGGCACACGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTGATGGCACAAGGCTTAGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment