Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU90_RS14920 Genome accession   NZ_AP025508
Coordinates   3368024..3369133 (-) Length   369 a.a.
NCBI ID   WP_004735479.1    Uniprot ID   A0A1R3EZW5
Organism   Vibrio splendidus strain LMG 19031     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3363024..3374133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU90_RS14885 - 3363340..3363840 (+) 501 WP_012604825.1 SprT family zinc-dependent metalloprotease -
  OCU90_RS14890 - 3363938..3364669 (+) 732 WP_017084333.1 endonuclease -
  OCU90_RS14895 rsmE 3364829..3365560 (+) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU90_RS14900 gshB 3365570..3366520 (+) 951 WP_017077777.1 glutathione synthase -
  OCU90_RS14905 - 3366637..3367200 (+) 564 WP_017095267.1 YqgE/AlgH family protein -
  OCU90_RS14910 ruvX 3367274..3367696 (+) 423 WP_017084336.1 Holliday junction resolvase RuvX -
  OCU90_RS14915 - 3367796..3367948 (+) 153 WP_017084337.1 hypothetical protein -
  OCU90_RS14920 pilU 3368024..3369133 (-) 1110 WP_004735479.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU90_RS14925 pilT 3369148..3370185 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU90_RS14930 - 3370212..3370916 (+) 705 WP_061025919.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU90_RS14935 proC 3371029..3371847 (+) 819 WP_017077774.1 pyrroline-5-carboxylate reductase -
  OCU90_RS14940 - 3371923..3372480 (+) 558 WP_004735475.1 YggT family protein -
  OCU90_RS14945 yggU 3372510..3372800 (+) 291 WP_054541895.1 DUF167 family protein YggU -
  OCU90_RS14950 - 3372829..3373260 (+) 432 WP_004735472.1 DUF4426 domain-containing protein -
  OCU90_RS14955 - 3373309..3373908 (+) 600 WP_061025922.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41351.25 Da        Isoelectric Point: 5.8700

>NTDB_id=92322 OCU90_RS14920 WP_004735479.1 3368024..3369133(-) (pilU) [Vibrio splendidus strain LMG 19031]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVLAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92322 OCU90_RS14920 WP_004735479.1 3368024..3369133(-) (pilU) [Vibrio splendidus strain LMG 19031]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTATCTCAAAAAGCATCGGATCTTTATATCACCGTTGATGCGCCTATCCT
GTTTCGTGTCGATGGTGAACTGCGACCTCAAGGCGAGAAATTGAATGCGGCTCAGGTTGCTCAGTTACTTGATGCGATGA
TGGATCAAGACCGACGCGATGAATATCAGCAAACGCGAGAGGCTAATTTTGCCATCGTACGTGATTTTGGTCGTTTTCGT
GTGAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTCATTCGTCGTATCGAAACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTACTGCAAGACCTGTCAATCGCAAAGCGTGGATTGGTGCTGGTGGTTGGTGCTACGGGCTCTGGTA
AATCAACCTCAATGGCTGCGATGACGGGCTATCGTAATACCAATCGTTCAGGGCATATTTTGACGGTTGAAGATCCAATT
GAATTCGTTCACGAACATAAAAAATGTATCGTAACTCAACGTGAGGTTGGGCTAGACACCGAGAGCTATGAAGTTGCGCT
TAAGAACTCTTTACGCCAAGCACCGGATATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGCCACTTGTGTATGGCAACACTGCACGCGAATAATGCCAACCAAGCGTTAGAACGTATTCTTCAC
TTGGTGCCGAAAGAACAGAAAGAGCAGTTCTTGTTTGATCTGTCGATGAACCTGCGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGCAGCGGTCGCCATGGCGTGTTTGAGATTCTACTTAACAGCCCGCGAGTGTCTGACTTGATTCGTC
GTGGTGAGTTGCATGAACTAAAAGCGACTATGGCTAAATCGAAAGAGATTGGTATGCAGACTTTTGACCAAGCTTTGTAT
GACTTGGTGCTTGCAGGCAAGATCAGTGAAGAAGACGCATTCCACAGTGCTGATTCCGCGAATGACTTACGCTTAATGCT
AAAAACCAGACGTGGTGATGATGACTACGGAACCGGTGCTTTGGCTGGCGTGAAGATTGATATGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EZW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.511

99.729

0.843
  pilU Pseudomonas stutzeri DSM 10701

56.065

100

0.564
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363

Multiple sequence alignment