Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV12_RS13880 Genome accession   NZ_AP025506
Coordinates   3173819..3174856 (-) Length   345 a.a.
NCBI ID   WP_017629549.1    Uniprot ID   -
Organism   Vibrio pomeroyi strain LMG 20537     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3168819..3179856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV12_RS13850 rsmE 3169516..3170247 (+) 732 WP_261884896.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV12_RS13855 gshB 3170257..3171207 (+) 951 WP_261884897.1 glutathione synthase -
  OCV12_RS13860 - 3171317..3171880 (+) 564 WP_261884898.1 YqgE/AlgH family protein -
  OCV12_RS13865 ruvX 3171935..3172357 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  OCV12_RS13870 - 3172456..3172617 (+) 162 WP_261884899.1 hypothetical protein -
  OCV12_RS13875 pilU 3172695..3173804 (-) 1110 WP_261884900.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV12_RS13880 pilT 3173819..3174856 (-) 1038 WP_017629549.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV12_RS13885 - 3174883..3175587 (+) 705 WP_261884901.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV12_RS13890 proC 3175687..3176505 (+) 819 WP_261884902.1 pyrroline-5-carboxylate reductase -
  OCV12_RS13895 - 3176563..3177120 (+) 558 WP_010435653.1 YggT family protein -
  OCV12_RS13900 yggU 3177150..3177440 (+) 291 WP_261884903.1 DUF167 family protein YggU -
  OCV12_RS13905 - 3177469..3177900 (+) 432 WP_261884904.1 DUF4426 domain-containing protein -
  OCV12_RS13910 - 3177949..3178548 (+) 600 WP_261884905.1 XTP/dITP diphosphatase -
  OCV12_RS13915 hemW 3178548..3179735 (+) 1188 WP_261884906.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38257.78 Da        Isoelectric Point: 6.0507

>NTDB_id=92278 OCV12_RS13880 WP_017629549.1 3173819..3174856(-) (pilT) [Vibrio pomeroyi strain LMG 20537]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLDQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVERKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=92278 OCV12_RS13880 WP_017629549.1 3173819..3174856(-) (pilT) [Vibrio pomeroyi strain LMG 20537]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCATCGTTTAGTTTTTGAGA
TCATGAGCGATTCACAGCGTGGTGAGTTTGAAGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGT
TTCCGTGTTAACGCTTTTAACCAAGCTCGTGGTTGCTCCGCTGTCTTTCGAACTATCCCTGTAGAGATCCCGACTTTAGA
TCAGTTAGGTGCGCCTGAAATCTTTGAAAAGATTGCGAACTACGAAAAAGGTTTAGTGCTCGTTACTGGCCCTACTGGTT
CTGGTAAATCGACAACGCTCGCAGCGATGGTTGATTACGTAAACCGTAACCACAATAAGCACATCCTGACGATTGAAGAC
CCGATTGAATTCGTTCATACCAACAATAAATGCCTTGTTAACCAGCGTGAGGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGCTGCGCAGTGCGTTACGTGAAGACCCAGATGTAATTCTTGTTGGTGAGCTTCGTGACCAAGAGACGATCAGCTTGG
CGCTAACCGCAGCGGAAACCGGCCACTTGGTTTTTGGTACTCTGCATACCAGCTCTGCTGCTAAAACTATCGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGCTGTCTGAATCGTTGCGTTCGGTCATCGCTCAGAA
GTTGTTGAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCTATTAGAAACTTGATTC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGCGCAGCACATGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTAATGGCTCAAGGCTTGGTTGACTCGGAAGAGGTCGAGAGAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.899

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.609

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.44

98.841

0.499
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.812

0.394
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment