Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV12_RS13875 Genome accession   NZ_AP025506
Coordinates   3172695..3173804 (-) Length   369 a.a.
NCBI ID   WP_261884900.1    Uniprot ID   -
Organism   Vibrio pomeroyi strain LMG 20537     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3167695..3178804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV12_RS13840 - 3167979..3168479 (+) 501 WP_261884894.1 SprT family zinc-dependent metalloprotease -
  OCV12_RS13845 - 3168631..3169362 (+) 732 WP_261884895.1 endonuclease -
  OCV12_RS13850 rsmE 3169516..3170247 (+) 732 WP_261884896.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV12_RS13855 gshB 3170257..3171207 (+) 951 WP_261884897.1 glutathione synthase -
  OCV12_RS13860 - 3171317..3171880 (+) 564 WP_261884898.1 YqgE/AlgH family protein -
  OCV12_RS13865 ruvX 3171935..3172357 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  OCV12_RS13870 - 3172456..3172617 (+) 162 WP_261884899.1 hypothetical protein -
  OCV12_RS13875 pilU 3172695..3173804 (-) 1110 WP_261884900.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV12_RS13880 pilT 3173819..3174856 (-) 1038 WP_017629549.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV12_RS13885 - 3174883..3175587 (+) 705 WP_261884901.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV12_RS13890 proC 3175687..3176505 (+) 819 WP_261884902.1 pyrroline-5-carboxylate reductase -
  OCV12_RS13895 - 3176563..3177120 (+) 558 WP_010435653.1 YggT family protein -
  OCV12_RS13900 yggU 3177150..3177440 (+) 291 WP_261884903.1 DUF167 family protein YggU -
  OCV12_RS13905 - 3177469..3177900 (+) 432 WP_261884904.1 DUF4426 domain-containing protein -
  OCV12_RS13910 - 3177949..3178548 (+) 600 WP_261884905.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41381.33 Da        Isoelectric Point: 5.8696

>NTDB_id=92277 OCV12_RS13875 WP_261884900.1 3172695..3173804(-) (pilU) [Vibrio pomeroyi strain LMG 20537]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGDKLNAAQIAQLLDAMMDQDRRDEYQQTREANFAIVREFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGCGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVIAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92277 OCV12_RS13875 WP_261884900.1 3172695..3173804(-) (pilU) [Vibrio pomeroyi strain LMG 20537]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTGTCTCAAAAAGCATCGGATCTTTATATCACCGTTGATGCACCTATCCT
GTTTCGTGTTGATGGTGAATTACGACCTCAAGGCGATAAGCTGAATGCGGCTCAGATTGCTCAATTACTTGATGCGATGA
TGGATCAAGACCGACGTGATGAATATCAGCAAACACGTGAGGCAAACTTTGCCATTGTGCGTGAGTTTGGTCGCTTTCGT
GTCAGTGCATTCTTCCAACGAGAGCTACCTGGAGCGGTAATTCGACGCATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTATTACAAGACCTTTCAATCGCTAAGCGGGGGCTTGTGCTGGTGGTTGGTGCGACTGGCTCTGGTA
AATCGACCTCAATGGCCGCAATGACAGGCTATCGCAATACCAATCGTTCAGGGCACATCTTAACGGTTGAAGACCCGATT
GAATTCGTCCATGAACATAAGAAATGTATTGTGACTCAGCGTGAGGTCGGGCTCGACACCGAGAGCTACGAAGTCGCACT
TAAGAACTCGTTACGTCAAGCACCAGATATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCTACACTGCATGCGAATAACGCTAACCAAGCACTAGAGCGTATTCTCCAC
TTGGTGCCAAAAGAGCAGAAAGAACAGTTCCTGTTTGATCTGTCGATGAATCTGCGTGGTGTGGTCGCTCAACAGCTAAT
TCGAGATAAGAATGGCTGCGGTCGTCATGGTGTGTTTGAGATTTTACTCAACAGCCCCCGAGTGTCTGACCTAATTCGTC
GTGGTGAGCTGCATGAACTAAAAGCAACGATGGCGAAATCGAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GATTTAGTTATAGCAGGCAAGATCAGCGAAGAGGATGCGTTCCACAGTGCTGACTCCGCTAACGACTTACGCTTGATGCT
AAAAACCAGACGTGGCGATGATGACTACGGGACCGGTGCTTTGGCTGGTGTGAAGATCGATATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.696

99.729

0.835
  pilU Pseudomonas stutzeri DSM 10701

56.065

100

0.564
  pilU Acinetobacter baylyi ADP1

55.271

95.122

0.526
  pilT Legionella pneumophila strain Lp02

41.888

91.87

0.385
  pilT Legionella pneumophila strain ERS1305867

41.888

91.87

0.385
  pilT Acinetobacter baumannii strain A118

41.279

93.225

0.385
  pilT Acinetobacter baumannii D1279779

41.279

93.225

0.385
  pilT Acinetobacter nosocomialis M2

41.279

93.225

0.385
  pilT Pseudomonas aeruginosa PAK

41.298

91.87

0.379
  pilT Acinetobacter baylyi ADP1

41.867

89.973

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.593

87.805

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Pseudomonas stutzeri DSM 10701

40

90.786

0.363
  pilT Neisseria gonorrhoeae MS11

38.551

93.496

0.36
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36

Multiple sequence alignment