Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   vsple_RS11995 Genome accession   NZ_AP025503
Coordinates   2741294..2742331 (-) Length   345 a.a.
NCBI ID   WP_261882113.1    Uniprot ID   -
Organism   Vibrio pelagius strain ATCC 25916     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2736294..2747331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  vsple_RS11965 rsmE 2737053..2737784 (+) 732 WP_150869304.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  vsple_RS11970 gshB 2737798..2738748 (+) 951 WP_261882109.1 glutathione synthase -
  vsple_RS11975 - 2738842..2739405 (+) 564 WP_261882110.1 YqgE/AlgH family protein -
  vsple_RS11980 ruvX 2739437..2739859 (+) 423 WP_032549845.1 Holliday junction resolvase RuvX -
  vsple_RS11985 - 2739955..2740113 (+) 159 WP_261882111.1 hypothetical protein -
  vsple_RS11990 pilU 2740169..2741278 (-) 1110 WP_261882112.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  vsple_RS11995 pilT 2741294..2742331 (-) 1038 WP_261882113.1 type IV pilus twitching motility protein PilT Machinery gene
  vsple_RS12000 - 2742358..2743062 (+) 705 WP_255229873.1 YggS family pyridoxal phosphate-dependent enzyme -
  vsple_RS12005 proC 2743120..2743938 (+) 819 WP_261882114.1 pyrroline-5-carboxylate reductase -
  vsple_RS12010 - 2743993..2744550 (+) 558 WP_255229871.1 YggT family protein -
  vsple_RS12015 yggU 2744550..2744840 (+) 291 WP_255229870.1 DUF167 family protein YggU -
  vsple_RS12020 - 2744869..2745300 (+) 432 WP_255229869.1 DUF4426 domain-containing protein -
  vsple_RS12025 - 2745337..2745936 (+) 600 WP_239841281.1 XTP/dITP diphosphatase -
  vsple_RS12030 hemW 2745936..2747123 (+) 1188 WP_261882115.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38177.61 Da        Isoelectric Point: 6.0485

>NTDB_id=92237 vsple_RS11995 WP_261882113.1 2741294..2742331(-) (pilT) [Vibrio pelagius strain ATCC 25916]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPDIFEKIANFEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGVVDSDEVQNKIEIETALF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=92237 vsple_RS11995 WP_261882113.1 2741294..2742331(-) (pilT) [Vibrio pelagius strain ATCC 25916]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTTAGGAAGCTCGGAATACCGGCTTTGAGCCATGCTGATGTCCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAGCTGGAAGTCGACTTTTCATTTGAGTTGCCGAATGTTGGACGT
TTTCGTGTGAATGCATTTAACCAGTCGCGTGGTTGTTCTGCGGTGTTTCGAACTATCCCAGTTGAGATCCCAACACTCGA
GCAGTTAGGTGCTCCTGATATCTTTGAAAAGATTGCTAACTTTGAGAAAGGCTTGGTTTTAGTCACTGGCCCAACGGGTT
CAGGTAAATCGACGACACTGGCGGCGATGGTTGATTATGTAAACCGTAACCACAACAAACACATCCTTACTATTGAAGAC
CCTATCGAATTTGTTCATACCAACAATAAGTGCCTAGTGAACCAGCGTGAGGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGCTGCGCAGTGCACTGCGTGAAGACCCAGACGTTATTTTAGTGGGAGAGTTACGTGACCAAGAGACGATCAGCTTAG
CTTTAACTGCAGCTGAAACCGGCCACCTGGTGTTTGGTACGCTACATACTAGCTCTGCGGCTAAAACCGTTGACCGTATT
ATTGATGTGTTTCCAGGCAGTGATAAGGACATGGTGCGCTCAATGCTGTCTGAGTCATTGCGCTCGGTGATTGCTCAGAA
GCTACTGAAGCGAGTAGGCGGAGGTCGTGTCGCTTGTCATGAGATTATGATGGCAACACCAGCTATTCGTAACTTGATTC
GTGAGGATAAAGTCGCTCAGATGTATTCCATCATTCAAACAGGGGCTGCACATGGCATGCAAACAATGGAGCAAAATGCC
AAACAGTTGATTGCTCAAGGCGTGGTGGACTCAGATGAAGTCCAAAACAAAATTGAAATCGAAACAGCACTGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.565

100

0.896
  pilT Vibrio cholerae strain A1552

89.565

100

0.896
  pilT Acinetobacter baumannii D1279779

72.948

95.362

0.696
  pilT Acinetobacter baumannii strain A118

72.948

95.362

0.696
  pilT Acinetobacter nosocomialis M2

72.644

95.362

0.693
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Pseudomonas aeruginosa PAK

68.235

98.551

0.672
  pilT Legionella pneumophila strain Lp02

68.693

95.362

0.655
  pilT Legionella pneumophila strain ERS1305867

68.693

95.362

0.655
  pilT Neisseria meningitidis 8013

65.152

95.652

0.623
  pilT Neisseria gonorrhoeae MS11

62.941

98.551

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.599

96.812

0.49
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment