Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   vsple_RS11990 Genome accession   NZ_AP025503
Coordinates   2740169..2741278 (-) Length   369 a.a.
NCBI ID   WP_261882112.1    Uniprot ID   -
Organism   Vibrio pelagius strain ATCC 25916     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2735169..2746278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  vsple_RS11955 - 2735579..2736082 (+) 504 WP_261882107.1 SprT family zinc-dependent metalloprotease -
  vsple_RS11960 - 2736173..2736904 (+) 732 WP_261882108.1 endonuclease -
  vsple_RS11965 rsmE 2737053..2737784 (+) 732 WP_150869304.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  vsple_RS11970 gshB 2737798..2738748 (+) 951 WP_261882109.1 glutathione synthase -
  vsple_RS11975 - 2738842..2739405 (+) 564 WP_261882110.1 YqgE/AlgH family protein -
  vsple_RS11980 ruvX 2739437..2739859 (+) 423 WP_032549845.1 Holliday junction resolvase RuvX -
  vsple_RS11985 - 2739955..2740113 (+) 159 WP_261882111.1 hypothetical protein -
  vsple_RS11990 pilU 2740169..2741278 (-) 1110 WP_261882112.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  vsple_RS11995 pilT 2741294..2742331 (-) 1038 WP_261882113.1 type IV pilus twitching motility protein PilT Machinery gene
  vsple_RS12000 - 2742358..2743062 (+) 705 WP_255229873.1 YggS family pyridoxal phosphate-dependent enzyme -
  vsple_RS12005 proC 2743120..2743938 (+) 819 WP_261882114.1 pyrroline-5-carboxylate reductase -
  vsple_RS12010 - 2743993..2744550 (+) 558 WP_255229871.1 YggT family protein -
  vsple_RS12015 yggU 2744550..2744840 (+) 291 WP_255229870.1 DUF167 family protein YggU -
  vsple_RS12020 - 2744869..2745300 (+) 432 WP_255229869.1 DUF4426 domain-containing protein -
  vsple_RS12025 - 2745337..2745936 (+) 600 WP_239841281.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41373.12 Da        Isoelectric Point: 5.6473

>NTDB_id=92236 vsple_RS11990 WP_261882112.1 2740169..2741278(-) (pilU) [Vibrio pelagius strain ATCC 25916]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEVLNEDQVSQLLDAMMDQERRDEYRQTREANFAIVRDSGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNHNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRVSDLIRRGDLHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTQRGDGDYGSGSLAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92236 vsple_RS11990 WP_261882112.1 2740169..2741278(-) (pilU) [Vibrio pelagius strain ATCC 25916]
ATGGAATTGAACCAGATCCTCGAGGGAATGCTGTCGCAAAAGGCGTCCGATCTTTATATCACGGTTGACGCGCCAGTGTT
GTTTCGCGTGGATGGAGAGCTGCGTCCGCAAGGTGAAGTTCTCAATGAAGATCAAGTGTCACAGCTGCTAGACGCAATGA
TGGATCAAGAGCGACGTGACGAGTACCGTCAAACACGCGAGGCGAACTTTGCGATCGTTCGAGATAGTGGTCGCTTTCGT
GTCAGTGCCTTCTTCCAGAGGGAGCTCCCTGGTGCAGTTATTCGTCGTATTGAAACTAATATCCCAACCTTTGAGCAGTT
AAAGCTCCCGGATGTTTTACAAGACCTCTCAATCGCTAAGCGCGGCCTTGTGCTGGTGGTTGGTGCAACAGGTTCTGGTA
AGTCGACGTCGATGGCAGCGATGACGGGGTATCGCAACCATAATCGCTCAGGTCATATTCTTACGGTGGAAGACCCGATT
GAGTTTGTGCATGAGCACAAGAAGTGCATTGTCACCCAGCGTGAAGTGGGTTTAGATACAGAGAGCTACGAAATTGCGCT
TAAGAATTCGTTACGCCAAGCACCCGATATGATCTTGATTGGTGAGATTCGAAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGGCATCTATGTATGGCAACGCTACATGCAAATAATGCCAACCAAGCGTTAGAACGTATTCTTCAT
TTGGTGCCGAAAGAGCAGAAAGAGCAGTTCTTGTTTGACCTGTCGATGAACCTACGTGGCGTTGTGGCGCAGCAGCTCAT
ACGAGACAAAAATGGCAATGGTCGTCATGGTGTGTTTGAGATCTTACTCAACAGCCCACGTGTGTCAGATCTTATTCGTC
GTGGTGATTTACATGAGCTCAAAGCAACGATGGCAAAGTCGAAAGAGATTGGAATGCAGACTTTCGATCAAGCATTGTAT
GACTTAGTTGTTGCAGGGAAAATCAGTGAAGAGGATGCATTCCACAGTGCTGACTCGGCGAACGATCTCAGGTTAATGTT
AAAAACGCAGCGCGGTGACGGTGATTACGGCAGTGGTTCTCTGGCCGGCGTGAAAATTGATATGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.511

99.729

0.843
  pilU Pseudomonas stutzeri DSM 10701

55.795

100

0.561
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Legionella pneumophila strain Lp02

42.69

92.683

0.396
  pilT Legionella pneumophila strain ERS1305867

42.69

92.683

0.396
  pilT Acinetobacter baumannii strain A118

42.353

92.141

0.39
  pilT Acinetobacter baumannii D1279779

42.353

92.141

0.39
  pilT Acinetobacter nosocomialis M2

42.353

92.141

0.39
  pilT Acinetobacter baylyi ADP1

41.813

92.683

0.388
  pilT Pseudomonas aeruginosa PAK

41.593

91.87

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.593

87.805

0.374
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.506

94.309

0.363
  pilT Neisseria meningitidis 8013

38.506

94.309

0.363

Multiple sequence alignment