Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV49_RS02090 Genome accession   NZ_AP025499
Coordinates   416286..417395 (+) Length   369 a.a.
NCBI ID   WP_192852325.1    Uniprot ID   -
Organism   Vibrio lentus strain LMG 21034     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411286..422395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV49_RS02055 - 411521..412120 (-) 600 WP_017080776.1 XTP/dITP diphosphatase -
  OCV49_RS02060 - 412169..412600 (-) 432 WP_192852327.1 DUF4426 domain-containing protein -
  OCV49_RS02065 yggU 412629..412919 (-) 291 WP_009847703.1 DUF167 family protein YggU -
  OCV49_RS02070 - 412949..413506 (-) 558 WP_017067798.1 YggT family protein -
  OCV49_RS02075 proC 413584..414402 (-) 819 WP_192852326.1 pyrroline-5-carboxylate reductase -
  OCV49_RS02080 - 414503..415207 (-) 705 WP_065110764.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV49_RS02085 pilT 415234..416271 (+) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV49_RS02090 pilU 416286..417395 (+) 1110 WP_192852325.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV49_RS02095 - 417493..417654 (-) 162 WP_170960663.1 hypothetical protein -
  OCV49_RS02100 ruvX 417754..418176 (-) 423 WP_017062240.1 Holliday junction resolvase RuvX -
  OCV49_RS02105 - 418233..418796 (-) 564 WP_102279318.1 YqgE/AlgH family protein -
  OCV49_RS02110 gshB 418912..419862 (-) 951 WP_192852324.1 glutathione synthase -
  OCV49_RS02115 rsmE 419872..420603 (-) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV49_RS02120 - 420763..421494 (-) 732 WP_192852323.1 endonuclease -
  OCV49_RS02125 - 421592..422092 (-) 501 WP_017105106.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41383.24 Da        Isoelectric Point: 5.8689

>NTDB_id=92177 OCV49_RS02090 WP_192852325.1 416286..417395(+) (pilU) [Vibrio lentus strain LMG 21034]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNVAQVSQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGDLHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALSGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92177 OCV49_RS02090 WP_192852325.1 416286..417395(+) (pilU) [Vibrio lentus strain LMG 21034]
ATGGAATTGAATCAAATCCTTGAGGGGATGCTTTCTCAAAAGGCTTCGGATCTTTATATCACTGTCGATGCTCCAATCCT
GTTTCGTGTCGATGGTGAACTTCGCCCTCAGGGAGAGAAACTGAATGTGGCTCAAGTATCTCAGCTTCTTGATGCGATGA
TGGATCAAGACCGACGCGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCCATTGTGCGTGACTTTGGTCGTTTTCGT
GTCAGTGCATTCTTTCAACGAGAGCTACCTGGAGCGGTTATTCGACGTATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTGCTGCAAGACCTTTCAATCGCTAAGCGCGGACTTGTGCTGGTGGTTGGTGCGACTGGCTCTGGTA
AATCGACCTCGATGGCTGCGATGACAGGCTATCGCAATACCAATCGTTCAGGTCATATCCTAACGGTTGAAGACCCGATT
GAATTTGTCCATGAACATAAAAAATGTATCGTGACTCAGCGTGAGGTCGGGCTCGATACCGAGAGTTACGAAGTCGCACT
TAAGAACTCTTTGCGCCAAGCACCAGATATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACTATGGAATACGCGATGA
CCTTTGCTGAGACGGGTCACTTGTGTATGGCTACACTGCATGCGAATAACGCTAACCAAGCACTAGAGCGTATTCTTCAC
TTGGTACCAAAAGAGCAGAAAGAGCAGTTCCTGTTTGATCTGTCGATGAACCTGCGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGCAGCGGTCGTCATGGTGTGTTTGAGATTTTACTCAACAGCCCACGAGTGTCTGACTTAATTCGTC
GTGGTGACTTGCATGAGCTTAAAGCGACGATGGCTAAATCGAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GATTTAGTCGTTGCTGGCAAGATTAGCGAAGAAGATGCGTTCCACAGTGCAGATTCAGCCAATGACTTGCGCTTGATGTT
GAAAACCAGACGTGGCGATGATGACTACGGGACTGGCGCTTTGTCTGGCGTTAAGATTGATATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.783

99.729

0.846
  pilU Pseudomonas stutzeri DSM 10701

56.065

100

0.564
  pilU Acinetobacter baylyi ADP1

53.846

95.122

0.512
  pilT Legionella pneumophila strain Lp02

42.69

92.683

0.396
  pilT Legionella pneumophila strain ERS1305867

42.69

92.683

0.396
  pilT Acinetobacter baumannii strain A118

42.105

92.683

0.39
  pilT Acinetobacter baumannii D1279779

42.105

92.683

0.39
  pilT Acinetobacter nosocomialis M2

42.105

92.683

0.39
  pilT Acinetobacter baylyi ADP1

41.813

92.683

0.388
  pilT Pseudomonas aeruginosa PAK

41.593

91.87

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

87.805

0.377
  pilT Pseudomonas stutzeri DSM 10701

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.551

93.496

0.36
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36

Multiple sequence alignment