Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV49_RS02085 Genome accession   NZ_AP025499
Coordinates   415234..416271 (+) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio lentus strain LMG 21034     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 410234..421271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV49_RS02050 hemW 410334..411521 (-) 1188 WP_192852328.1 radical SAM family heme chaperone HemW -
  OCV49_RS02055 - 411521..412120 (-) 600 WP_017080776.1 XTP/dITP diphosphatase -
  OCV49_RS02060 - 412169..412600 (-) 432 WP_192852327.1 DUF4426 domain-containing protein -
  OCV49_RS02065 yggU 412629..412919 (-) 291 WP_009847703.1 DUF167 family protein YggU -
  OCV49_RS02070 - 412949..413506 (-) 558 WP_017067798.1 YggT family protein -
  OCV49_RS02075 proC 413584..414402 (-) 819 WP_192852326.1 pyrroline-5-carboxylate reductase -
  OCV49_RS02080 - 414503..415207 (-) 705 WP_065110764.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV49_RS02085 pilT 415234..416271 (+) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV49_RS02090 pilU 416286..417395 (+) 1110 WP_192852325.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV49_RS02095 - 417493..417654 (-) 162 WP_170960663.1 hypothetical protein -
  OCV49_RS02100 ruvX 417754..418176 (-) 423 WP_017062240.1 Holliday junction resolvase RuvX -
  OCV49_RS02105 - 418233..418796 (-) 564 WP_102279318.1 YqgE/AlgH family protein -
  OCV49_RS02110 gshB 418912..419862 (-) 951 WP_192852324.1 glutathione synthase -
  OCV49_RS02115 rsmE 419872..420603 (-) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=92176 OCV49_RS02085 WP_004735478.1 415234..416271(+) (pilT) [Vibrio lentus strain LMG 21034]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=92176 OCV49_RS02085 WP_004735478.1 415234..416271(+) (pilT) [Vibrio lentus strain LMG 21034]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCCGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGTGGTGAGTTCGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGT
TTCCGTGTTAATGCTTTTAATCAATCTCGTGGTTGCTCTGCTGTATTTCGTACCATTCCAGTAGAGATTCCTACCCTTGA
ACAGCTAGGAGCTCCTGAGATCTTTGAAAGAATATCTAATTACGAAAAAGGTTTAGTCTTGGTGACAGGGCCGACGGGTT
CAGGTAAGTCGACAACTTTGGCTGCGATGGTGGATTACGTTAACCGTAACCATAATAAGCATATATTGACGATTGAAGAC
CCGATCGAATTTGTTCATACCAACAACAAATGCTTAATCAACCAACGAGAAGTGCACCGCGACACTCACAGCTTCAAAGC
TGCGCTGCGCAGTGCGTTACGTGAAGATCCAGACGTTATCCTTGTTGGTGAGCTTCGTGACCAAGAGACGATCAGCTTAG
CGCTAACCGCAGCAGAAACGGGTCACTTAGTGTTTGGTACCTTGCACACGAGCTCTGCGGCAAAAACTATCGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGATATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATCGCCCAGAA
GCTGTTAAAGCGTGTCGGTGGTGGTCGTGTCGCTTGTCATGAGATCATGATGGCGACACCTGCGATCAGAAACTTGATCC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGCGCGGCACATGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGTTGATGGCTCAAGGCTTGGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCTATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment