Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VP504_RS13835 Genome accession   NZ_CP142108
Coordinates   2976267..2977304 (+) Length   345 a.a.
NCBI ID   WP_325891658.1    Uniprot ID   -
Organism   Grimontia sp. NTOU-MAR1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2971267..2982304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP504_RS13800 (VP504_13800) hemW 2971404..2972555 (-) 1152 WP_325891653.1 radical SAM family heme chaperone HemW -
  VP504_RS13805 (VP504_13805) - 2972562..2973161 (-) 600 WP_325891654.1 XTP/dITP diphosphatase -
  VP504_RS13810 (VP504_13810) - 2973251..2973685 (-) 435 WP_325891655.1 DUF4426 domain-containing protein -
  VP504_RS13815 (VP504_13815) yggU 2973780..2974091 (-) 312 WP_325891656.1 DUF167 family protein YggU -
  VP504_RS13820 (VP504_13820) - 2974095..2974661 (-) 567 WP_062705825.1 YggT family protein -
  VP504_RS13825 (VP504_13825) proC 2974671..2975489 (-) 819 WP_046304352.1 pyrroline-5-carboxylate reductase -
  VP504_RS13830 (VP504_13830) - 2975536..2976240 (-) 705 WP_325891657.1 YggS family pyridoxal phosphate-dependent enzyme -
  VP504_RS13835 (VP504_13835) pilT 2976267..2977304 (+) 1038 WP_325891658.1 type IV pilus twitching motility protein PilT Machinery gene
  VP504_RS13840 (VP504_13840) pilU 2977307..2978416 (+) 1110 WP_325891659.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VP504_RS13845 (VP504_13845) ruvX 2978468..2978899 (-) 432 WP_325891660.1 Holliday junction resolvase RuvX -
  VP504_RS13850 (VP504_13850) - 2978909..2979472 (-) 564 WP_325891661.1 YqgE/AlgH family protein -
  VP504_RS13855 (VP504_13855) gshB 2979583..2980530 (-) 948 WP_325891662.1 glutathione synthase -
  VP504_RS13860 (VP504_13860) rsmE 2980560..2981291 (-) 732 WP_325891663.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VP504_RS13865 (VP504_13865) - 2981367..2982059 (-) 693 WP_325894050.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38002.43 Da        Isoelectric Point: 6.5649

>NTDB_id=921746 VP504_RS13835 WP_325891658.1 2976267..2977304(+) (pilT) [Grimontia sp. NTOU-MAR1]
MDITELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLSLPPLDHNAVHRLIFDIMNDAQQREFEQNLEVDFSFELASVGR
FRVNAFHQSRGCAAVFRSIPSKIPTLDAINAPAVFQQFTEYQKGLVLVTGPTGSGKSTTLSAMIDKINSSQHRHVLTIED
PIEFVHEQKNCLINQREVHRDTHSFNNALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGADKPMVRSMLSESLRAVVSQKLVKQISGGRIAAHEVMIATPAIRNLIREDKIAQMYSMIQTGSSLGMLTMEQSL
RNLTMQGLVDTAEANKALDNAGVGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=921746 VP504_RS13835 WP_325891658.1 2976267..2977304(+) (pilT) [Grimontia sp. NTOU-MAR1]
ATGGATATTACTGAATTACTGGACTTTAGTGTAAAGCATAACGCTTCAGATCTACATCTTTCTGCAGGGGTTCCCCCAAT
GGTCCGCGTAGATGGTGATGTCCGCAAGCTTAGCCTGCCTCCGCTAGACCACAATGCTGTGCACCGTCTTATTTTCGATA
TCATGAACGATGCTCAGCAGCGCGAGTTTGAACAGAACCTTGAAGTTGACTTCTCTTTTGAGCTTGCCTCTGTCGGTCGC
TTCCGTGTCAATGCGTTTCACCAATCCCGAGGCTGTGCCGCGGTGTTCCGCAGCATTCCTTCGAAAATTCCAACACTTGA
TGCGATTAACGCACCAGCAGTGTTCCAACAATTTACCGAATATCAGAAAGGTCTGGTACTGGTCACCGGACCGACAGGCT
CAGGTAAGTCGACCACGTTGTCGGCGATGATAGATAAAATTAACAGCAGTCAGCATCGTCACGTATTGACCATCGAAGAC
CCGATTGAATTTGTCCACGAACAGAAAAACTGTCTGATCAACCAGCGTGAAGTGCACCGCGATACCCACTCCTTCAACAA
TGCGCTCCGGTCGGCACTGCGTGAAGACCCGGATGTGATTCTGGTTGGTGAGCTTCGCGATAAAGAAACCATTAGCCTTG
CGCTAACGGCAGCAGAAACCGGTCACTTGGTATTTGGTACGTTGCATACCTCCAGTGCGGCAAAAACCATCGACCGCATT
ATTGATGTTTTTCCAGGTGCGGATAAGCCGATGGTGCGCTCCATGCTGTCCGAATCGCTACGCGCGGTGGTTTCGCAAAA
ACTAGTGAAGCAGATTTCCGGCGGCCGTATTGCTGCTCACGAAGTCATGATTGCAACACCGGCTATCCGAAATCTTATCC
GTGAAGACAAAATTGCCCAGATGTATTCGATGATCCAGACAGGCTCCAGTCTTGGCATGCTGACGATGGAACAGTCACTT
CGAAATCTCACCATGCAAGGGTTGGTGGATACAGCTGAAGCAAATAAAGCACTGGATAATGCGGGAGTTGGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.336

98.261

0.73
  pilT Vibrio cholerae strain A1552

74.336

98.261

0.73
  pilT Pseudomonas aeruginosa PAK

68.452

97.391

0.667
  pilT Pseudomonas stutzeri DSM 10701

68.155

97.391

0.664
  pilT Acinetobacter baumannii D1279779

67.656

97.681

0.661
  pilT Acinetobacter baumannii strain A118

67.656

97.681

0.661
  pilT Acinetobacter nosocomialis M2

67.656

97.681

0.661
  pilT Acinetobacter baylyi ADP1

67.665

96.812

0.655
  pilT Legionella pneumophila strain Lp02

65.598

99.42

0.652
  pilT Legionella pneumophila strain ERS1305867

65.598

99.42

0.652
  pilT Neisseria meningitidis 8013

62.573

99.13

0.62
  pilT Neisseria gonorrhoeae MS11

62.281

99.13

0.617
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

95.652

0.478
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

40.058

99.13

0.397
  pilU Vibrio cholerae strain A1552

39.766

99.13

0.394