Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VP504_RS13840 Genome accession   NZ_CP142108
Coordinates   2977307..2978416 (+) Length   369 a.a.
NCBI ID   WP_325891659.1    Uniprot ID   -
Organism   Grimontia sp. NTOU-MAR1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2972307..2983416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP504_RS13805 (VP504_13805) - 2972562..2973161 (-) 600 WP_325891654.1 XTP/dITP diphosphatase -
  VP504_RS13810 (VP504_13810) - 2973251..2973685 (-) 435 WP_325891655.1 DUF4426 domain-containing protein -
  VP504_RS13815 (VP504_13815) yggU 2973780..2974091 (-) 312 WP_325891656.1 DUF167 family protein YggU -
  VP504_RS13820 (VP504_13820) - 2974095..2974661 (-) 567 WP_062705825.1 YggT family protein -
  VP504_RS13825 (VP504_13825) proC 2974671..2975489 (-) 819 WP_046304352.1 pyrroline-5-carboxylate reductase -
  VP504_RS13830 (VP504_13830) - 2975536..2976240 (-) 705 WP_325891657.1 YggS family pyridoxal phosphate-dependent enzyme -
  VP504_RS13835 (VP504_13835) pilT 2976267..2977304 (+) 1038 WP_325891658.1 type IV pilus twitching motility protein PilT Machinery gene
  VP504_RS13840 (VP504_13840) pilU 2977307..2978416 (+) 1110 WP_325891659.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VP504_RS13845 (VP504_13845) ruvX 2978468..2978899 (-) 432 WP_325891660.1 Holliday junction resolvase RuvX -
  VP504_RS13850 (VP504_13850) - 2978909..2979472 (-) 564 WP_325891661.1 YqgE/AlgH family protein -
  VP504_RS13855 (VP504_13855) gshB 2979583..2980530 (-) 948 WP_325891662.1 glutathione synthase -
  VP504_RS13860 (VP504_13860) rsmE 2980560..2981291 (-) 732 WP_325891663.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VP504_RS13865 (VP504_13865) - 2981367..2982059 (-) 693 WP_325894050.1 endonuclease -
  VP504_RS13870 (VP504_13870) - 2982190..2982681 (-) 492 WP_325891664.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40997.00 Da        Isoelectric Point: 6.2358

>NTDB_id=921747 VP504_RS13840 WP_325891659.1 2977307..2978416(+) (pilU) [Grimontia sp. NTOU-MAR1]
MQDTLQKLLDKMVAQKASDCYITVDAPSMLRVDGSLKSVGEALDLEGVKTLVNEAMDAETEQKFYADREANFALVTDNGR
FRVSAFWQRDLPGMVLRRIETKIPEIQSLGLPDVIQDLSIAKRGLVLIVGATGSGKSTSMAAMVGFRNRHRSGHILTVED
PIEFIHPHGKCIVTQREIGIDTDSYEVALKNSLRQAPDMIMIGEIRTQETMEYAMQFAETGHLCIATLHANNANQALERV
LHLVPKERREQFLFDLSLNLRGILAQQLVPDVSGIGRHGAYELLLNTSHVSDLIRRGELHELKETIRKGKNAGMCTFDQS
LFELFQAGKVSKDEALHHADSRNDLKLMMKMAEQSSAGLSLANVSIEGQ

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=921747 VP504_RS13840 WP_325891659.1 2977307..2978416(+) (pilU) [Grimontia sp. NTOU-MAR1]
ATGCAGGACACGCTCCAAAAACTTCTGGATAAGATGGTGGCGCAGAAGGCGTCAGATTGTTATATCACGGTCGACGCTCC
TAGTATGTTGCGGGTCGATGGCAGTTTAAAGTCTGTGGGAGAAGCACTTGATCTCGAAGGTGTGAAAACGCTTGTTAATG
AAGCGATGGATGCCGAGACTGAACAGAAATTTTATGCTGACCGCGAAGCCAACTTTGCTTTGGTGACCGATAATGGACGA
TTTCGTGTCAGTGCGTTCTGGCAGCGTGATTTGCCGGGCATGGTGCTTCGTCGTATCGAAACTAAAATCCCTGAAATTCA
GTCATTAGGCTTGCCTGATGTGATTCAGGATTTGTCGATAGCCAAACGTGGTCTGGTGCTGATTGTCGGTGCGACGGGGT
CAGGTAAATCGACGTCGATGGCAGCGATGGTGGGTTTTCGTAACCGACACCGCAGCGGCCATATCCTGACGGTGGAAGAC
CCAATTGAGTTTATCCACCCACATGGCAAATGCATCGTGACCCAGCGTGAAATCGGCATTGATACCGACAGCTACGAAGT
GGCGCTGAAAAACTCGCTGCGTCAGGCGCCGGATATGATCATGATTGGTGAGATCCGAACGCAGGAAACCATGGAATACG
CCATGCAGTTTGCTGAAACCGGTCACCTTTGTATCGCAACCCTACACGCCAACAATGCTAATCAGGCACTGGAAAGAGTG
CTGCATTTGGTACCGAAAGAGCGTCGCGAACAGTTCCTGTTTGATTTGTCGCTTAACCTTCGCGGTATTCTGGCCCAGCA
ACTGGTGCCAGACGTCAGTGGTATCGGACGTCATGGCGCATACGAGCTTCTGCTCAATACTTCCCATGTGTCGGATCTGA
TTCGCCGTGGTGAACTGCATGAGCTGAAAGAAACGATCCGCAAAGGCAAGAACGCGGGAATGTGTACCTTTGACCAATCC
CTGTTTGAGTTGTTTCAGGCGGGCAAGGTTTCTAAAGACGAAGCACTCCACCACGCGGACTCGCGAAATGATCTCAAACT
CATGATGAAAATGGCTGAGCAGTCATCTGCCGGACTTTCGCTGGCGAATGTCAGCATTGAGGGGCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

65.659

98.645

0.648
  pilU Pseudomonas stutzeri DSM 10701

54.821

98.374

0.539
  pilU Acinetobacter baylyi ADP1

50.986

96.206

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.665

97.019

0.385