Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV56_RS13800 Genome accession   NZ_AP025492
Coordinates   3108708..3109745 (-) Length   345 a.a.
NCBI ID   WP_048605840.1    Uniprot ID   A0AA86X1P3
Organism   Vibrio gigantis strain LMG 22741     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3103708..3114745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV56_RS13770 rsmE 3104408..3105139 (+) 732 WP_086712017.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV56_RS13775 gshB 3105149..3106099 (+) 951 WP_017629553.1 glutathione synthase -
  OCV56_RS13780 - 3106209..3106772 (+) 564 WP_086712018.1 YqgE/AlgH family protein -
  OCV56_RS13785 ruvX 3106826..3107248 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  OCV56_RS13790 - 3107348..3107509 (+) 162 WP_167373144.1 hypothetical protein -
  OCV56_RS13795 pilU 3107584..3108693 (-) 1110 WP_086712019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV56_RS13800 pilT 3108708..3109745 (-) 1038 WP_048605840.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV56_RS13805 - 3109772..3110476 (+) 705 WP_086712020.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV56_RS13810 proC 3110561..3111379 (+) 819 WP_086712021.1 pyrroline-5-carboxylate reductase -
  OCV56_RS13815 - 3111437..3111994 (+) 558 WP_010435653.1 YggT family protein -
  OCV56_RS13820 yggU 3112024..3112314 (+) 291 WP_086712022.1 DUF167 family protein YggU -
  OCV56_RS13825 - 3112343..3112774 (+) 432 WP_016768173.1 DUF4426 domain-containing protein -
  OCV56_RS13830 - 3112823..3113422 (+) 600 WP_086712023.1 XTP/dITP diphosphatase -
  OCV56_RS13835 hemW 3113422..3114609 (+) 1188 WP_086712024.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38243.79 Da        Isoelectric Point: 6.0512

>NTDB_id=92107 OCV56_RS13800 WP_048605840.1 3108708..3109745(-) (pilT) [Vibrio gigantis strain LMG 22741]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLEQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=92107 OCV56_RS13800 WP_048605840.1 3108708..3109745(-) (pilT) [Vibrio gigantis strain LMG 22741]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAGTTTGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTCCGTGTGAATGCTTTTAACCAAGCTCGTGGTTGCTCGGCTGTCTTTCGAACTATCCCTGTAGAGATCCCAACTTTAGA
ACAGTTAGGTGCGCCTGAAATCTTTGAAAAGATTGCTAATTACGAAAAAGGTTTGGTACTTGTCACTGGACCCACTGGTT
CTGGTAAATCGACGACGCTCGCGGCGATGGTGGATTACGTAAATCGCAACCACAATAAGCACATCCTGACCATCGAAGAC
CCGATTGAATTCGTTCATACCAACAATAAATGCCTTGTTAACCAGCGTGAAGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGCTACGCAGTGCGTTACGTGAAGACCCAGACGTTATCCTTGTAGGTGAGCTTCGTGACCAAGAAACCATTAGTTTAG
CGTTAACCGCTGCAGAAACCGGTCACTTGGTTTTTGGTACTTTGCATACCAGTTCGGCGGCAAAAACTATTGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGCTCAATGCTTTCTGAATCATTGCGTTCGGTCATTGCTCAGAA
GCTGTTAAAACGTGTAGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCAGCGATTAGAAACTTGATTC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGGGCGGCACATGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTGATGGCGCAAGGCTTGGTTGACTCGGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.275

100

0.893
  pilT Vibrio cholerae strain A1552

89.275

100

0.893
  pilT Acinetobacter baumannii D1279779

72.289

96.232

0.696
  pilT Acinetobacter baumannii strain A118

72.289

96.232

0.696
  pilT Acinetobacter nosocomialis M2

71.988

96.232

0.693
  pilT Pseudomonas aeruginosa PAK

69.118

98.551

0.681
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Acinetobacter baylyi ADP1

71.212

95.652

0.681
  pilT Legionella pneumophila strain Lp02

65.797

100

0.658
  pilT Legionella pneumophila strain ERS1305867

65.797

100

0.658
  pilT Neisseria meningitidis 8013

62.899

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.609

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.385

94.203

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.812

0.397
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment