Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV56_RS13795 Genome accession   NZ_AP025492
Coordinates   3107584..3108693 (-) Length   369 a.a.
NCBI ID   WP_086712019.1    Uniprot ID   A0A5M9NBB1
Organism   Vibrio gigantis strain LMG 22741     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3102584..3113693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV56_RS13760 - 3102870..3103370 (+) 501 WP_086712015.1 SprT family zinc-dependent metalloprotease -
  OCV56_RS13765 - 3103523..3104254 (+) 732 WP_086712016.1 endonuclease -
  OCV56_RS13770 rsmE 3104408..3105139 (+) 732 WP_086712017.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV56_RS13775 gshB 3105149..3106099 (+) 951 WP_017629553.1 glutathione synthase -
  OCV56_RS13780 - 3106209..3106772 (+) 564 WP_086712018.1 YqgE/AlgH family protein -
  OCV56_RS13785 ruvX 3106826..3107248 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  OCV56_RS13790 - 3107348..3107509 (+) 162 WP_167373144.1 hypothetical protein -
  OCV56_RS13795 pilU 3107584..3108693 (-) 1110 WP_086712019.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV56_RS13800 pilT 3108708..3109745 (-) 1038 WP_048605840.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV56_RS13805 - 3109772..3110476 (+) 705 WP_086712020.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV56_RS13810 proC 3110561..3111379 (+) 819 WP_086712021.1 pyrroline-5-carboxylate reductase -
  OCV56_RS13815 - 3111437..3111994 (+) 558 WP_010435653.1 YggT family protein -
  OCV56_RS13820 yggU 3112024..3112314 (+) 291 WP_086712022.1 DUF167 family protein YggU -
  OCV56_RS13825 - 3112343..3112774 (+) 432 WP_016768173.1 DUF4426 domain-containing protein -
  OCV56_RS13830 - 3112823..3113422 (+) 600 WP_086712023.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41340.22 Da        Isoelectric Point: 5.8700

>NTDB_id=92106 OCV56_RS13795 WP_086712019.1 3107584..3108693(-) (pilU) [Vibrio gigantis strain LMG 22741]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKSGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTSALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92106 OCV56_RS13795 WP_086712019.1 3107584..3108693(-) (pilU) [Vibrio gigantis strain LMG 22741]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTGTCTCAAAAGGCGTCGGATCTTTATATCACCGTTGATGCGCCTATCTT
GTTTCGTGTTGATGGTGAATTGCGACCTCAAGGAGAGAAGCTGAATGCGGCTCAGGTTGCCCAACTACTTGATGCGATGA
TGGATCAAGACAGACGTGATGAATATCAGCAAACACGTGAAGCTAACTTTGCTATCGTGCGTGATTTTGGTCGTTTTCGT
GTTAGTGCATTCTTCCAACGAGAGCTACCTGGAGCAGTCATTCGACGCATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTACTGCAAGACCTTTCAATCGCCAAGCGCGGACTTGTGCTGGTGGTTGGTGCGACTGGATCTGGTA
AATCGACCTCAATGGCTGCAATGACAGGTTATCGCAATACCAATCGCTCGGGGCATATCTTAACGGTTGAAGACCCGATT
GAATTCGTCCATGAGCATAAAAAATGTATCGTGACTCAGCGTGAGGTCGGGCTCGACACCGAGAGCTACGAAGTCGCACT
TAAGAACTCGTTACGCCAAGCACCAGATATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATATGCGATGA
CCTTTGCTGAGACGGGTCACCTGTGTATGGCAACTTTGCACGCGAATAATGCTAACCAAGCGTTAGAGCGTATCCTTCAT
TTGGTGCCGAAAGAGCAGAAAGAACAGTTCCTGTTTGATCTGTCGATGAACCTTCGTGGTGTGGTCGCTCAACAGTTAAT
CAGAGACAAGAGTGGCAGTGGGCGTCATGGTGTGTTTGAGATTCTACTGAACAGCCCACGCGTGTCTGATTTGATTCGTC
GCGGTGAGTTGCATGAGTTAAAAGCAACGATGGCAAAATCGAAAGAGATTGGTATGCAGACCTTTGATCAAGCTTTGTAT
GATTTAGTTGTTGCAGGTAAGATCAGCGAAGAAGACGCGTTCCACAGTGCCGATTCGGCGAATGATTTGCGCTTAATGCT
CAAAACCAGACGTGGAGATGATGACTACGGAACGAGTGCATTGGCCGGTGTGAAGATTGACATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9NBB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

99.729

0.84
  pilU Pseudomonas stutzeri DSM 10701

56.604

100

0.569
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain Lp02

41.888

91.87

0.385
  pilT Legionella pneumophila strain ERS1305867

41.888

91.87

0.385
  pilT Acinetobacter baumannii D1279779

41.52

92.683

0.385
  pilT Acinetobacter nosocomialis M2

41.52

92.683

0.385
  pilT Acinetobacter baumannii strain A118

41.52

92.683

0.385
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363

Multiple sequence alignment