Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M3O54_RS07295 Genome accession   NZ_CP142004
Coordinates   1658267..1659304 (+) Length   345 a.a.
NCBI ID   WP_064632554.1    Uniprot ID   A0A3E1KFD3
Organism   Xanthomonas nasturtii strain 8853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653267..1664304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3O54_RS07265 (M3O54_007250) - 1653586..1654128 (-) 543 WP_064632548.1 hypothetical protein -
  M3O54_RS07270 (M3O54_007255) - 1654186..1654527 (-) 342 WP_064632550.1 DUF3861 domain-containing protein -
  M3O54_RS07275 (M3O54_007260) - 1654585..1655223 (-) 639 WP_064632613.1 M23 family metallopeptidase -
  M3O54_RS07280 (M3O54_007265) - 1655333..1655758 (-) 426 WP_064632551.1 HU family DNA-binding protein -
  M3O54_RS07285 (M3O54_007270) proC 1656305..1657159 (-) 855 WP_064632552.1 pyrroline-5-carboxylate reductase -
  M3O54_RS07290 (M3O54_007275) - 1657223..1657915 (-) 693 WP_064632553.1 YggS family pyridoxal phosphate-dependent enzyme -
  M3O54_RS07295 (M3O54_007280) pilT 1658267..1659304 (+) 1038 WP_064632554.1 type IV pilus twitching motility protein PilT Machinery gene
  M3O54_RS07300 (M3O54_007285) pilU 1659418..1660548 (+) 1131 WP_064632557.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M3O54_RS07305 (M3O54_007290) - 1660864..1661538 (+) 675 WP_064632559.1 YitT family protein -
  M3O54_RS07310 (M3O54_007295) - 1661671..1662317 (-) 647 Protein_1425 DUF72 domain-containing protein -
  M3O54_RS07315 (M3O54_007300) - 1662354..1662926 (-) 573 WP_064632615.1 DNA-3-methyladenine glycosylase I -
  M3O54_RS07320 (M3O54_007305) - 1663217..1663783 (+) 567 WP_064632563.1 YqgE/AlgH family protein -
  M3O54_RS07325 (M3O54_007310) ruvX 1663776..1664243 (+) 468 WP_064632565.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38666.33 Da        Isoelectric Point: 6.7269

>NTDB_id=920840 M3O54_RS07295 WP_064632554.1 1658267..1659304(+) (pilT) [Xanthomonas nasturtii strain 8853]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRHQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=920840 M3O54_RS07295 WP_064632554.1 1658267..1659304(+) (pilT) [Xanthomonas nasturtii strain 8853]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGTTGGACCACAAGCAGGTGCATGCGCTGGTCTACGACA
TCATGTCCGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCCTTCGAGATTCCCTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGCACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATTTTCCGCCAGTTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGGT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACTGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCGTTGCGTGAAGACCCGGACATCATTCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTTG
CGCTGACCGCCGCGGAAACCGGCCATTTGGTGTTCGGCACCTTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCCCTGCGCGCGGTGATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGCACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCGATCCAGACAGGCCAGCAGTACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCCACCAGGCGCGCGAATACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1KFD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394