Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M3O54_RS07300 Genome accession   NZ_CP142004
Coordinates   1659418..1660548 (+) Length   376 a.a.
NCBI ID   WP_064632557.1    Uniprot ID   -
Organism   Xanthomonas nasturtii strain 8853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1654418..1665548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3O54_RS07275 (M3O54_007260) - 1654585..1655223 (-) 639 WP_064632613.1 M23 family metallopeptidase -
  M3O54_RS07280 (M3O54_007265) - 1655333..1655758 (-) 426 WP_064632551.1 HU family DNA-binding protein -
  M3O54_RS07285 (M3O54_007270) proC 1656305..1657159 (-) 855 WP_064632552.1 pyrroline-5-carboxylate reductase -
  M3O54_RS07290 (M3O54_007275) - 1657223..1657915 (-) 693 WP_064632553.1 YggS family pyridoxal phosphate-dependent enzyme -
  M3O54_RS07295 (M3O54_007280) pilT 1658267..1659304 (+) 1038 WP_064632554.1 type IV pilus twitching motility protein PilT Machinery gene
  M3O54_RS07300 (M3O54_007285) pilU 1659418..1660548 (+) 1131 WP_064632557.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M3O54_RS07305 (M3O54_007290) - 1660864..1661538 (+) 675 WP_064632559.1 YitT family protein -
  M3O54_RS07310 (M3O54_007295) - 1661671..1662317 (-) 647 Protein_1425 DUF72 domain-containing protein -
  M3O54_RS07315 (M3O54_007300) - 1662354..1662926 (-) 573 WP_064632615.1 DNA-3-methyladenine glycosylase I -
  M3O54_RS07320 (M3O54_007305) - 1663217..1663783 (+) 567 WP_064632563.1 YqgE/AlgH family protein -
  M3O54_RS07325 (M3O54_007310) ruvX 1663776..1664243 (+) 468 WP_064632565.1 Holliday junction resolvase RuvX -
  M3O54_RS07330 (M3O54_007315) - 1664257..1665204 (+) 948 WP_064632567.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41931.15 Da        Isoelectric Point: 6.7681

>NTDB_id=920841 M3O54_RS07300 WP_064632557.1 1659418..1660548(+) (pilU) [Xanthomonas nasturtii strain 8853]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKDVMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=920841 M3O54_RS07300 WP_064632557.1 1659418..1660548(+) (pilU) [Xanthomonas nasturtii strain 8853]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGATCTGTTCATCACTTCGGGCAT
GCCGCCGGCAATCAAGGTGAATGGCAAGATCAGCCCGATCACCCAGACACCGCTGACGGCGCAGCAGAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAATGCAACTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTGAGCTGCTTTTATCAGCGCAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGTG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCGACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGATAG
CTGGGAAAATGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCACGCCGTGGCCTTTGCCGAAACTGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAATGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAACCAGCTGTTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGT
TGCGCAGCAACTGATTCCGACCCCGGACGGGCGCAGCCGCCGCGTGGCGATGGAAATCATGTTGGGCACCCCGCTGGTGC
AGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGATGTCATGAAGGAGTCCACCAACCTGGGCATGCGCACTTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGTGAAATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCTTGCGCATCAAGCTCTCCCAGGGTGGCGATGCCAAGACCCTGGCACAGGGGCTGGATGGCGTGGAGATTGCCG
AGGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.302

97.606

0.657
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362