Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU78_RS02195 Genome accession   NZ_AP025490
Coordinates   489255..490364 (+) Length   369 a.a.
NCBI ID   WP_137374473.1    Uniprot ID   -
Organism   Vibrio gallaecicus strain CECT 7244     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484255..495364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU78_RS02160 - 484406..485005 (-) 600 WP_137374466.1 XTP/dITP diphosphatase -
  OCU78_RS02165 - 485056..485487 (-) 432 WP_137374467.1 DUF4426 domain-containing protein -
  OCU78_RS02170 yggU 485517..485807 (-) 291 WP_137374468.1 DUF167 family protein YggU -
  OCU78_RS02175 - 485872..486429 (-) 558 WP_137374469.1 YggT family protein -
  OCU78_RS02180 proC 486490..487308 (-) 819 WP_137374470.1 pyrroline-5-carboxylate reductase -
  OCU78_RS02185 - 487381..488085 (-) 705 WP_137374471.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU78_RS02190 pilT 488112..489143 (+) 1032 WP_137374472.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU78_RS02195 pilU 489255..490364 (+) 1110 WP_137374473.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU78_RS02200 ruvX 490437..490862 (-) 426 WP_137374474.1 Holliday junction resolvase RuvX -
  OCU78_RS02205 - 490931..491494 (-) 564 WP_137374475.1 YqgE/AlgH family protein -
  OCU78_RS02210 gshB 491594..492544 (-) 951 WP_137374476.1 glutathione synthase -
  OCU78_RS02215 rsmE 492554..493285 (-) 732 WP_137374477.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU78_RS02220 degS 493464..494525 (-) 1062 WP_137374589.1 outer membrane-stress sensor serine endopeptidase DegS -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41382.17 Da        Isoelectric Point: 6.0673

>NTDB_id=92041 OCU78_RS02195 WP_137374473.1 489255..490364(+) (pilU) [Vibrio gallaecicus strain CECT 7244]
MELNQILEGMISQKASDLYITVDAPILFRVDGELRPQGEKLNEAQVAQLLDAMMDQDRRAEYRRTRESNFAVVRDTGRFR
VSAFFQRELPGAVIRRIETNIPSFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNSNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGDLHELKATMAKSKEVGMQTFDQSLY
DLVVSGKISEEDAFHSADSANDLRLMLKTQRGDDSYGTGALAGVKIDME

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92041 OCU78_RS02195 WP_137374473.1 489255..490364(+) (pilU) [Vibrio gallaecicus strain CECT 7244]
ATGGAATTGAATCAAATTCTCGAAGGGATGATCTCGCAAAAAGCATCGGATCTTTATATCACGGTGGATGCACCTATATT
ATTTAGAGTTGACGGGGAGTTGCGCCCTCAAGGTGAAAAGCTCAATGAAGCTCAAGTTGCTCAGTTGCTTGATGCCATGA
TGGATCAAGATCGCCGTGCTGAGTATCGTCGAACTCGTGAATCAAATTTTGCGGTAGTTCGAGATACTGGGCGTTTTCGT
GTGAGTGCTTTCTTCCAGAGGGAATTGCCTGGTGCTGTCATTCGCCGTATCGAAACCAATATCCCTAGCTTTGAGCAGCT
TAAACTTCCTGATGTACTCCAAGACCTATCTATCGCTAAGCGTGGGTTAGTGCTGGTGGTAGGTGCTACAGGGTCGGGTA
AATCAACTTCTATGGCGGCAATGACAGGTTATCGTAATTCTAACCGCTCAGGGCATATCCTAACGGTTGAAGACCCAATC
GAGTTTGTTCATGAGCACAAAAAATGTATCGTTACTCAGCGTGAAGTTGGGTTAGATACAGATAGTTATGAAGTTGCTTT
AAAGAACTCGCTTCGACAAGCGCCTGATATGATTCTTATTGGTGAAATACGTAGCCGTGAAACGATGGAATATGCCATGA
CTTTCGCGGAAACTGGTCACCTTTGTATGGCGACACTTCATGCCAATAATGCTAACCAAGCGTTAGAGCGTATTTTGCAT
TTAGTGCCAAAAGAGCAAAAAGAGCAATTCTTATTCGACTTATCAATGAACTTGCGCGGTGTGGTTGCTCAGCAACTTAT
CCGAGATAAAAATGGTAATGGTCGACATGGTGTGTTTGAGATTCTGCTCAATAGCCCAAGGATCTCTGATTTGATTCGCC
GCGGTGATCTTCATGAATTGAAGGCGACGATGGCGAAATCGAAAGAAGTTGGTATGCAAACTTTTGATCAATCATTATAT
GACTTAGTTGTTTCAGGGAAAATCAGTGAAGAGGATGCATTTCATAGTGCTGATTCTGCCAATGATCTACGCTTGATGCT
CAAGACCCAACGCGGTGATGACAGCTACGGAACCGGTGCACTTGCAGGTGTGAAGATTGATATGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.011

100

0.84
  pilU Pseudomonas stutzeri DSM 10701

55.526

100

0.558
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Legionella pneumophila strain Lp02

43.068

91.87

0.396
  pilT Legionella pneumophila strain ERS1305867

43.068

91.87

0.396
  pilT Acinetobacter baumannii strain A118

41.593

91.87

0.382
  pilT Acinetobacter baumannii D1279779

41.593

91.87

0.382
  pilT Acinetobacter nosocomialis M2

41.593

91.87

0.382
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Acinetobacter baylyi ADP1

41.39

89.702

0.371
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.975

87.805

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.551

93.496

0.36
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36

Multiple sequence alignment