Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU78_RS02190 Genome accession   NZ_AP025490
Coordinates   488112..489143 (+) Length   343 a.a.
NCBI ID   WP_137374472.1    Uniprot ID   -
Organism   Vibrio gallaecicus strain CECT 7244     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483112..494143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU78_RS02155 hemW 483216..484406 (-) 1191 WP_137374465.1 radical SAM family heme chaperone HemW -
  OCU78_RS02160 - 484406..485005 (-) 600 WP_137374466.1 XTP/dITP diphosphatase -
  OCU78_RS02165 - 485056..485487 (-) 432 WP_137374467.1 DUF4426 domain-containing protein -
  OCU78_RS02170 yggU 485517..485807 (-) 291 WP_137374468.1 DUF167 family protein YggU -
  OCU78_RS02175 - 485872..486429 (-) 558 WP_137374469.1 YggT family protein -
  OCU78_RS02180 proC 486490..487308 (-) 819 WP_137374470.1 pyrroline-5-carboxylate reductase -
  OCU78_RS02185 - 487381..488085 (-) 705 WP_137374471.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU78_RS02190 pilT 488112..489143 (+) 1032 WP_137374472.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU78_RS02195 pilU 489255..490364 (+) 1110 WP_137374473.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU78_RS02200 ruvX 490437..490862 (-) 426 WP_137374474.1 Holliday junction resolvase RuvX -
  OCU78_RS02205 - 490931..491494 (-) 564 WP_137374475.1 YqgE/AlgH family protein -
  OCU78_RS02210 gshB 491594..492544 (-) 951 WP_137374476.1 glutathione synthase -
  OCU78_RS02215 rsmE 492554..493285 (-) 732 WP_137374477.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38080.58 Da        Isoelectric Point: 6.2336

>NTDB_id=92040 OCU78_RS02190 WP_137374472.1 488112..489143(+) (pilT) [Vibrio gallaecicus strain CECT 7244]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFSHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPSVGR
FRVNAFNQARGCSAVFRTIPVEIPTLDQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNYNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNS
KQLIAQGLVDPEEVQKKIETSLF

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=92040 OCU78_RS02190 WP_137374472.1 488112..489143(+) (pilT) [Vibrio gallaecicus strain CECT 7244]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAGTTCCTGCTTTTAGTCATGCTGATGTACATCGTTTGGTTTTTGAGA
TCATGAACGATTCCCAGCGCAGTGAATTTGAAGAAAAGCTGGAAGTCGATTTTTCTTTTGAATTACCTAGTGTTGGACGC
TTCCGTGTTAATGCATTTAATCAGGCTCGTGGCTGTTCTGCTGTATTTCGTACCATTCCCGTTGAGATCCCAACCTTAGA
TCAATTAGGTGCGCCAGAGATCTTTGAAAAAATTGCAAATTATGAAAAGGGCTTAGTACTCGTTACGGGTCCTACTGGTT
CTGGTAAGTCGACCACACTTGCCGCTATGGTAGATTATGTAAACCGTAACTATAACAAACACATATTAACCATCGAAGAC
CCGATTGAATTCGTACATACCAACAATAAGTGTTTGATAAACCAACGTGAAGTACATAGAGATACCCATAGCTTTAAAGC
AGCCTTACGCAGTGCTTTACGTGAAGACCCTGATGTTATTTTGGTGGGCGAGCTTCGTGATCAAGAAACGATCAGTTTAG
CGCTTACAGCAGCCGAAACGGGGCACCTTGTTTTTGGTACATTGCATACTAGCTCTGCGGCAAAAACCATTGACCGTATT
ATTGATGTATTCCCCGGTAGTGATAAAGACATGGTTCGGTCTATGCTTTCTGAATCACTTCGTTCGGTTATTGCTCAGAA
GTTGCTTAAGCGTATTGGGGGCGGGCGTGTTGCTTGCCACGAAATTATGATGGCGACGCCAGCAATTCGTAACTTGATAC
GTGAAGATAAAGTGGCGCAGATGTACTCAATTATCCAAACGGGAGCTGCTCACGGCATGCAAACAATGGAACAAAACTCC
AAGCAGTTGATTGCTCAAGGTTTGGTGGATCCAGAAGAAGTTCAGAAGAAAATTGAAACATCGTTATTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

90.643

99.708

0.904
  pilT Vibrio cholerae strain A1552

90.643

99.708

0.904
  pilT Acinetobacter baumannii D1279779

72.289

96.793

0.7
  pilT Acinetobacter baumannii strain A118

72.289

96.793

0.7
  pilT Acinetobacter nosocomialis M2

71.988

96.793

0.697
  pilT Acinetobacter baylyi ADP1

71.212

96.21

0.685
  pilT Pseudomonas aeruginosa PAK

69.139

98.251

0.679
  pilT Pseudomonas stutzeri DSM 10701

69.139

98.251

0.679
  pilT Legionella pneumophila strain Lp02

67.173

95.918

0.644
  pilT Legionella pneumophila strain ERS1305867

67.173

95.918

0.644
  pilT Neisseria meningitidis 8013

63.45

99.708

0.633
  pilT Neisseria gonorrhoeae MS11

63.158

99.708

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

92.711

0.49
  pilU Vibrio cholerae strain A1552

42.769

94.752

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.719

97.376

0.397
  pilU Acinetobacter baylyi ADP1

40

93.294

0.373

Multiple sequence alignment