Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV50_RS12310 Genome accession   NZ_AP025487
Coordinates   2815568..2816605 (-) Length   345 a.a.
NCBI ID   WP_239841276.1    Uniprot ID   -
Organism   Vibrio fortis strain LMG 21557     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2810568..2821605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV50_RS12280 rsmE 2811325..2812056 (+) 732 WP_261903173.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV50_RS12285 gshB 2812070..2813020 (+) 951 WP_261903174.1 glutathione synthase -
  OCV50_RS12290 - 2813115..2813678 (+) 564 WP_239841272.1 YqgE/AlgH family protein -
  OCV50_RS12295 ruvX 2813710..2814132 (+) 423 WP_261903175.1 Holliday junction resolvase RuvX -
  OCV50_RS12300 - 2814229..2814387 (+) 159 WP_239841274.1 hypothetical protein -
  OCV50_RS12305 pilU 2814443..2815552 (-) 1110 WP_261903176.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV50_RS12310 pilT 2815568..2816605 (-) 1038 WP_239841276.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV50_RS12315 - 2816632..2817336 (+) 705 WP_239841277.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV50_RS12320 proC 2817394..2818212 (+) 819 WP_261903177.1 pyrroline-5-carboxylate reductase -
  OCV50_RS12325 - 2818267..2818824 (+) 558 WP_239841279.1 YggT family protein -
  OCV50_RS12330 yggU 2818824..2819114 (+) 291 WP_239841280.1 DUF167 family protein YggU -
  OCV50_RS12335 - 2819143..2819574 (+) 432 WP_032549854.1 DUF4426 domain-containing protein -
  OCV50_RS12340 - 2819613..2820212 (+) 600 WP_239841281.1 XTP/dITP diphosphatase -
  OCV50_RS12345 hemW 2820212..2821399 (+) 1188 WP_261903178.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38245.77 Da        Isoelectric Point: 6.0891

>NTDB_id=92018 OCV50_RS12310 WP_239841276.1 2815568..2816605(-) (pilT) [Vibrio fortis strain LMG 21557]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPDIFEKIANFEKGLVLVTGPTGSGKSTTLAAMVDYVNRNYNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGVVDSEEVQKKIEIETALF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=92018 OCV50_RS12310 WP_239841276.1 2815568..2816605(-) (pilT) [Vibrio fortis strain LMG 21557]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTTAGGAAGCTTGGAATACCGGCTTTGAGTCATGCTGATGTTCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTCGAAGAGAAGCTAGAAGTCGATTTTTCATTTGAGCTACCGAATGTTGGTCGC
TTTCGTGTGAATGCGTTTAACCAATCGCGCGGTTGTTCTGCGGTATTTCGAACCATCCCTGTTGAAATCCCAACACTGGA
GCAGTTAGGCGCTCCTGATATCTTTGAAAAGATCGCTAATTTTGAGAAAGGCTTGGTGTTAGTTACAGGTCCAACGGGGT
CGGGTAAGTCAACGACACTGGCGGCGATGGTTGATTATGTAAACCGTAACTACAACAAACACATCCTTACCATTGAAGAC
CCTATCGAATTTGTTCACACCAACAACAAGTGTCTTGTGAACCAGCGTGAAGTTCATCGTGATACTCACAGCTTTAAAGC
GGCTCTTCGAAGCGCCCTACGTGAAGACCCAGATGTTATCTTGGTCGGCGAGCTGCGTGACCAAGAGACCATCAGCTTAG
CGTTGACCGCAGCCGAAACTGGTCACCTAGTTTTTGGCACGCTGCATACGAGCTCTGCGGCTAAAACCATTGACCGTATT
ATTGATGTCTTCCCTGGCAGCGATAAGGACATGGTGCGCTCTATGCTTTCTGAATCTTTACGCTCGGTGATTGCTCAGAA
GTTATTGAAGCGAGTAGGCGGTGGGCGTGTCGCTTGTCATGAAATCATGATGGCAACGCCAGCAATCCGTAACTTGATTC
GTGAGGACAAAGTCGCTCAGATGTACTCGATCATTCAAACAGGTGCTGCACACGGCATGCAGACCATGGAGCAAAACGCG
AAACAGTTGATTGCTCAGGGAGTGGTTGACTCAGAAGAGGTCCAAAAGAAAATTGAAATCGAAACAGCGCTGTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.565

100

0.896
  pilT Vibrio cholerae strain A1552

89.565

100

0.896
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Acinetobacter baylyi ADP1

71.212

95.652

0.681
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Legionella pneumophila strain Lp02

68.997

95.362

0.658
  pilT Legionella pneumophila strain ERS1305867

68.997

95.362

0.658
  pilT Neisseria meningitidis 8013

63.235

98.551

0.623
  pilT Neisseria gonorrhoeae MS11

62.941

98.551

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

92.174

0.487
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.617

96.812

0.403
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment