Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV50_RS12305 Genome accession   NZ_AP025487
Coordinates   2814443..2815552 (-) Length   369 a.a.
NCBI ID   WP_261903176.1    Uniprot ID   -
Organism   Vibrio fortis strain LMG 21557     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2809443..2820552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV50_RS12270 - 2809851..2810354 (+) 504 WP_261903171.1 SprT family zinc-dependent metalloprotease -
  OCV50_RS12275 - 2810445..2811176 (+) 732 WP_261903172.1 endonuclease -
  OCV50_RS12280 rsmE 2811325..2812056 (+) 732 WP_261903173.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV50_RS12285 gshB 2812070..2813020 (+) 951 WP_261903174.1 glutathione synthase -
  OCV50_RS12290 - 2813115..2813678 (+) 564 WP_239841272.1 YqgE/AlgH family protein -
  OCV50_RS12295 ruvX 2813710..2814132 (+) 423 WP_261903175.1 Holliday junction resolvase RuvX -
  OCV50_RS12300 - 2814229..2814387 (+) 159 WP_239841274.1 hypothetical protein -
  OCV50_RS12305 pilU 2814443..2815552 (-) 1110 WP_261903176.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV50_RS12310 pilT 2815568..2816605 (-) 1038 WP_239841276.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV50_RS12315 - 2816632..2817336 (+) 705 WP_239841277.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV50_RS12320 proC 2817394..2818212 (+) 819 WP_261903177.1 pyrroline-5-carboxylate reductase -
  OCV50_RS12325 - 2818267..2818824 (+) 558 WP_239841279.1 YggT family protein -
  OCV50_RS12330 yggU 2818824..2819114 (+) 291 WP_239841280.1 DUF167 family protein YggU -
  OCV50_RS12335 - 2819143..2819574 (+) 432 WP_032549854.1 DUF4426 domain-containing protein -
  OCV50_RS12340 - 2819613..2820212 (+) 600 WP_239841281.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41280.08 Da        Isoelectric Point: 5.6664

>NTDB_id=92017 OCV50_RS12305 WP_261903176.1 2814443..2815552(-) (pilU) [Vibrio fortis strain LMG 21557]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEVLNAIQVSQLLDAMMDQERRDEYRQTREANFAIVRDSGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNSNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGDLHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTQRGDDDYGSGSLAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92017 OCV50_RS12305 WP_261903176.1 2814443..2815552(-) (pilU) [Vibrio fortis strain LMG 21557]
ATGGAATTAAATCAGATCCTCGAAGGCATGCTGTCACAAAAAGCGTCCGATCTTTATATTACGGTTGATGCACCTGTGCT
GTTTCGTGTCGATGGTGAGTTGCGCCCGCAAGGTGAAGTGCTCAACGCGATTCAAGTGTCACAGCTTCTTGATGCAATGA
TGGATCAAGAACGACGTGACGAGTACCGTCAAACGCGCGAGGCAAACTTTGCTATTGTTCGAGACAGTGGTCGCTTTCGT
GTCAGTGCTTTCTTTCAAAGAGAGCTACCAGGAGCCGTGATTCGTCGCATTGAAACGAATATTCCAACCTTTGAGCAATT
AAAGCTCCCTGATGTTCTGCAAGATCTCTCCATCGCTAAACGCGGTCTTGTGCTGGTGGTTGGTGCAACAGGCTCTGGTA
AGTCGACGTCTATGGCAGCAATGACAGGCTACCGTAATAGCAATCGCTCTGGTCATATCCTCACGGTAGAAGACCCAATT
GAGTTTGTGCATGAGCATAAGAAGTGCATTGTCACCCAGCGTGAGGTGGGCTTAGATACTGAAAGCTATGAGGTGGCACT
GAAGAACTCGTTACGTCAAGCGCCAGATATGATCTTGATTGGTGAAATTCGAAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCATCTTTGTATGGCCACATTGCACGCCAACAATGCTAACCAAGCGTTAGAACGTATTCTTCAC
TTGGTCCCTAAAGAGCAAAAAGAGCAGTTCTTGTTCGACCTGTCGATGAACCTACGGGGTGTCGTCGCTCAGCAGTTAAT
TCGTGATAAAAATGGCAGTGGTCGTCATGGTGTGTTTGAGATCTTACTCAACAGCCCACGTGTATCTGATCTGATTCGTC
GTGGTGATTTGCATGAGCTCAAAGCAACGATGGCCAAATCAAAAGAGATCGGCATGCAGACTTTTGACCAAGCTTTATAT
GACTTAGTCGTAGCAGGGAAAATCAGTGAAGAGGATGCATTCCACAGTGCTGATTCTGCAAACGACCTACGCTTGATGCT
GAAAACTCAGCGTGGTGACGATGACTACGGCAGCGGATCCTTGGCTGGTGTTAAGATTGATATGGGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.783

99.729

0.846
  pilU Pseudomonas stutzeri DSM 10701

58.286

94.851

0.553
  pilU Acinetobacter baylyi ADP1

53.561

95.122

0.509
  pilT Legionella pneumophila strain Lp02

42.982

92.683

0.398
  pilT Legionella pneumophila strain ERS1305867

42.982

92.683

0.398
  pilT Acinetobacter baumannii strain A118

42.353

92.141

0.39
  pilT Acinetobacter baumannii D1279779

42.353

92.141

0.39
  pilT Acinetobacter nosocomialis M2

42.353

92.141

0.39
  pilT Acinetobacter baylyi ADP1

42.136

91.328

0.385
  pilT Pseudomonas aeruginosa PAK

41.593

91.87

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

87.805

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.118

91.87

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.506

94.309

0.363
  pilT Neisseria meningitidis 8013

38.506

94.309

0.363

Multiple sequence alignment