Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV36_RS02310 Genome accession   NZ_AP025483
Coordinates   472339..473448 (+) Length   369 a.a.
NCBI ID   WP_135457236.1    Uniprot ID   -
Organism   Vibrio echinoideorum strain DSM 107264     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467339..478448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV36_RS02275 - 467584..468183 (-) 600 WP_135457240.1 XTP/dITP diphosphatase -
  OCV36_RS02280 - 468232..468663 (-) 432 WP_017075428.1 DUF4426 domain-containing protein -
  OCV36_RS02285 yggU 468692..468982 (-) 291 WP_017075427.1 DUF167 family protein YggU -
  OCV36_RS02290 - 469012..469569 (-) 558 WP_017075426.1 YggT family protein -
  OCV36_RS02295 proC 469653..470471 (-) 819 WP_135457238.1 pyrroline-5-carboxylate reductase -
  OCV36_RS02300 - 470556..471260 (-) 705 WP_102553300.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV36_RS02305 pilT 471287..472324 (+) 1038 WP_017075423.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV36_RS02310 pilU 472339..473448 (+) 1110 WP_135457236.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV36_RS02315 - 473526..473687 (-) 162 WP_167853042.1 hypothetical protein -
  OCV36_RS02320 ruvX 473785..474207 (-) 423 WP_017075420.1 Holliday junction resolvase RuvX -
  OCV36_RS02325 - 474255..474818 (-) 564 WP_135457234.1 YqgE/AlgH family protein -
  OCV36_RS02330 gshB 474936..475886 (-) 951 WP_135457232.1 glutathione synthase -
  OCV36_RS02335 rsmE 475896..476627 (-) 732 WP_102553296.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV36_RS02340 - 476788..477519 (-) 732 WP_017075416.1 endonuclease -
  OCV36_RS02345 - 477617..478117 (-) 501 WP_017075415.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41297.26 Da        Isoelectric Point: 6.2490

>NTDB_id=91954 OCV36_RS02310 WP_135457236.1 472339..473448(+) (pilU) [Vibrio echinoideorum strain DSM 107264]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEKLNAAQVAQLLDAMMDQDRRDEYKQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKSTMAKSKEIGMQTFDQALY
DLVVAGKICEEDAFHSADSANDLRLMLKTRRGDGDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=91954 OCV36_RS02310 WP_135457236.1 472339..473448(+) (pilU) [Vibrio echinoideorum strain DSM 107264]
ATGGAATTGAATCAAATCCTTGAAGGTATGCTTTCACAAAAAGCGTCGGATCTTTACATCACGGTAGATGCTCCGGTTCT
ATTTCGTGTTGATGGTGAATTGCGACCTCAAGGGGAGAAGCTTAATGCGGCTCAGGTTGCTCAGTTACTTGATGCGATGA
TGGATCAAGATAGGCGTGACGAGTACAAACAAACGCGTGAGGCTAATTTTGCCATTGTGCGCGATTTTGGTCGCTTTCGT
GTCAGTGCTTTTTTTCAACGAGAGTTACCTGGAGCGGTCATTCGACGTATCGAAACCAACATCCCAACCTTCGAGCAGTT
AAAGCTTCCTGATGTCTTACAAGACCTTTCAATAGCAAAGCGTGGACTTGTGCTGGTGGTTGGGGCGACAGGCTCGGGTA
AATCAACCTCAATGGCTGCGATGACGGGTTATCGCAATACTAATCGTTCGGGGCATATCTTGACGGTTGAAGATCCGATT
GAGTTCGTGCACGAACATAAAAAGTGTATTGTGACTCAGCGAGAAGTGGGGCTCGACACTGAAAGTTATGAAGTCGCGCT
TAAGAACTCATTACGCCAAGCGCCAGATATGATCTTAATTGGTGAAATCCGTAGTCGTGAAACCATGGAATATGCGATGA
CGTTTGCTGAAACTGGTCATCTTTGTATGGCGACTTTACATGCCAATAATGCCAATCAAGCGTTAGAACGTATTCTTCAC
CTGGTGCCTAAAGAGCAAAAAGAACAGTTCTTATTTGATCTGTCGATGAACCTACGTGGTGTGGTCGCTCAGCAGCTTAT
TCGAGATAAGAATGGCAGTGGTCGTCATGGTGTGTTCGAAATCCTACTCAACAGCCCACGAGTATCTGATTTGATTCGTA
GAGGTGAGCTACATGAACTCAAGTCGACAATGGCTAAATCAAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GACTTAGTCGTAGCAGGCAAGATCTGTGAAGAGGATGCGTTCCACAGTGCTGACTCCGCTAATGACTTACGCTTAATGCT
AAAAACCAGACGCGGTGATGGTGACTACGGAACTGGTGCTTTGGCTGGCGTGAAGATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

99.729

0.84
  pilU Pseudomonas stutzeri DSM 10701

56.604

100

0.569
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.519

87.805

0.382
  pilT Pseudomonas aeruginosa PAK

41.593

91.87

0.382
  pilT Acinetobacter baylyi ADP1

42.598

89.702

0.382
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.212

89.431

0.369
  pilT Vibrio cholerae strain A1552

41.212

89.431

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363

Multiple sequence alignment