Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV36_RS02305 Genome accession   NZ_AP025483
Coordinates   471287..472324 (+) Length   345 a.a.
NCBI ID   WP_017075423.1    Uniprot ID   A0A2N7JML0
Organism   Vibrio echinoideorum strain DSM 107264     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466287..477324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV36_RS02270 hemW 466397..467584 (-) 1188 WP_135457242.1 radical SAM family heme chaperone HemW -
  OCV36_RS02275 - 467584..468183 (-) 600 WP_135457240.1 XTP/dITP diphosphatase -
  OCV36_RS02280 - 468232..468663 (-) 432 WP_017075428.1 DUF4426 domain-containing protein -
  OCV36_RS02285 yggU 468692..468982 (-) 291 WP_017075427.1 DUF167 family protein YggU -
  OCV36_RS02290 - 469012..469569 (-) 558 WP_017075426.1 YggT family protein -
  OCV36_RS02295 proC 469653..470471 (-) 819 WP_135457238.1 pyrroline-5-carboxylate reductase -
  OCV36_RS02300 - 470556..471260 (-) 705 WP_102553300.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV36_RS02305 pilT 471287..472324 (+) 1038 WP_017075423.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV36_RS02310 pilU 472339..473448 (+) 1110 WP_135457236.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV36_RS02315 - 473526..473687 (-) 162 WP_167853042.1 hypothetical protein -
  OCV36_RS02320 ruvX 473785..474207 (-) 423 WP_017075420.1 Holliday junction resolvase RuvX -
  OCV36_RS02325 - 474255..474818 (-) 564 WP_135457234.1 YqgE/AlgH family protein -
  OCV36_RS02330 gshB 474936..475886 (-) 951 WP_135457232.1 glutathione synthase -
  OCV36_RS02335 rsmE 475896..476627 (-) 732 WP_102553296.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38240.79 Da        Isoelectric Point: 6.0501

>NTDB_id=91953 OCV36_RS02305 WP_017075423.1 471287..472324(+) (pilT) [Vibrio echinoideorum strain DSM 107264]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMDDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPVEIPTLEQLGAPDIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIQTSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=91953 OCV36_RS02305 WP_017075423.1 471287..472324(+) (pilT) [Vibrio echinoideorum strain DSM 107264]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTTAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCATCGTTTAGTTTTTGAGA
TCATGGACGACTCACAGCGCGGTGAGTTTGAGGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTCCGTGTTAATGCTTTTAACCAAGCTCGTGGTTGCGCAGCTGTCTTTCGAACTATCCCTGTAGAAATTCCCACGTTAGA
GCAGTTAGGCGCACCTGATATTTTTGAAAAGATTGCCAACTACGAAAAAGGCTTAGTGCTGGTGACTGGCCCTACAGGTT
CTGGTAAATCGACAACTTTGGCGGCAATGGTCGATTACGTAAATCGCAACCACAACAAGCATATTCTGACGATTGAAGAT
CCGATCGAATTTGTGCATACCAATAATAAATGTCTCGTTAATCAGCGTGAAGTTCATCGTGATACTCACAGCTTCAAAGC
GGCGCTGCGCAGTGCGTTACGTGAAGACCCGGACGTTATCCTTGTCGGTGAGCTTCGTGACCAAGAAACGATTAGCTTGG
CGCTAACAGCAGCAGAAACTGGGCATTTAGTGTTTGGTACTCTACACACCAGTTCTGCGGCTAAAACTATCGATCGTATT
ATCGATGTATTCCCAGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTAAAGCGTGTCGGTGGTGGTCGTGTGGCTTGTCATGAAATTATGATGGCGACACCTGCGATCAGAAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTACTCGATCATTCAAACGGGCGCTGCACATGGTATGCAAACCATGGAGCAAAATGCG
AAGCAGCTTATGGCTCAAGGTTTGGTTGATTCGGAAGAGGTCGAGAAAAAGATCGAAATTCAAACCTCTATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7JML0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.289

96.232

0.696
  pilT Acinetobacter baumannii strain A118

72.289

96.232

0.696
  pilT Acinetobacter nosocomialis M2

71.988

96.232

0.693
  pilT Acinetobacter baylyi ADP1

71.212

95.652

0.681
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Legionella pneumophila strain Lp02

66.087

100

0.661
  pilT Legionella pneumophila strain ERS1305867

66.087

100

0.661
  pilT Neisseria meningitidis 8013

63.188

100

0.632
  pilT Neisseria gonorrhoeae MS11

62.899

100

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.385

94.203

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment