Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VA613_RS12875 Genome accession   NZ_CP141769
Coordinates   2571631..2572674 (+) Length   347 a.a.
NCBI ID   WP_324779416.1    Uniprot ID   -
Organism   Thiobacillus sedimenti strain SCUT-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2566631..2577674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VA613_RS12850 (VA613_12850) - 2567209..2569113 (-) 1905 WP_324779411.1 dynamin family protein -
  VA613_RS12855 (VA613_12855) - 2569110..2569421 (-) 312 WP_324779412.1 DUF167 domain-containing protein -
  VA613_RS12860 (VA613_12860) - 2569459..2570034 (-) 576 WP_324779413.1 YggT family protein -
  VA613_RS12865 (VA613_12865) proC 2570031..2570846 (-) 816 WP_324779414.1 pyrroline-5-carboxylate reductase -
  VA613_RS12870 (VA613_12870) - 2570839..2571564 (-) 726 WP_324779415.1 YggS family pyridoxal phosphate-dependent enzyme -
  VA613_RS12875 (VA613_12875) pilT 2571631..2572674 (+) 1044 WP_324779416.1 type IV pilus twitching motility protein PilT Machinery gene
  VA613_RS12880 (VA613_12880) pilU 2572685..2573812 (+) 1128 WP_324779417.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VA613_RS12885 (VA613_12885) - 2573904..2574287 (-) 384 WP_324779418.1 hypothetical protein -
  VA613_RS12890 (VA613_12890) - 2574372..2575622 (-) 1251 WP_324779419.1 THUMP domain-containing protein -
  VA613_RS12895 (VA613_12895) - 2575642..2576055 (-) 414 WP_324779420.1 CopD family protein -
  VA613_RS12900 (VA613_12900) - 2576054..2576590 (+) 537 WP_324779421.1 TlpA disulfide reductase family protein -
  VA613_RS12905 (VA613_12905) - 2576587..2576790 (+) 204 WP_324779422.1 SlyX family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38280.94 Da        Isoelectric Point: 6.7773

>NTDB_id=919316 VA613_RS12875 WP_324779416.1 2571631..2572674(+) (pilT) [Thiobacillus sedimenti strain SCUT-2]
MDISELLAFAVKNKASDLHLSAGLPPMIRVNGDIRRINLPPMDHKDVHRMVYDIMNDSQRKVYEETLEIDFSFEIPSLAR
FRVNAFNQQYGAGAVFRTIPSKVLTLEELNAPPIFKEISDQPRGIVLVTGPTGSGKSTTLAAMMDYVNENQYAHILTVED
PIEFVHQSKKCLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGAGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTGGNLGMQTLDQN
LQDLVRRNIVAPGVARAAAQNKEAFTG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=919316 VA613_RS12875 WP_324779416.1 2571631..2572674(+) (pilT) [Thiobacillus sedimenti strain SCUT-2]
GTGGATATTTCTGAACTGCTGGCATTCGCCGTCAAGAACAAGGCATCGGACTTGCACCTCTCGGCGGGCCTGCCGCCGAT
GATCCGGGTCAACGGCGACATCCGCCGCATCAACCTGCCGCCGATGGACCACAAGGACGTGCACCGCATGGTGTACGACA
TCATGAACGACAGCCAGCGCAAGGTGTACGAGGAGACGCTGGAAATCGACTTCTCGTTCGAGATTCCCTCGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGCAGTACGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGACGCTCGA
GGAGCTCAACGCGCCGCCCATCTTCAAGGAGATCTCGGACCAGCCGCGCGGCATCGTGCTGGTCACCGGCCCGACCGGCT
CCGGCAAGTCGACCACGCTGGCGGCGATGATGGACTACGTCAACGAGAACCAGTACGCCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACCAGAGCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGCATACGCTGTCGTTCAACAA
CGCGCTGCGCTCGGCGCTGCGTGAGGACCCGGACGTCATCCTGGTCGGCGAATTGCGCGACCTCGAAACGATCCGCCTGG
CGCTCACCGCCGCCGAGACCGGCCACCTCGTGTTCGGCACCCTGCACACCTCGTCGGCCGCCAAGACGATCGACCGCGTG
GTCGACGTGTTCCCCGCGGCGGAAAAGGAAATGGTGCGCTCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCGCAGAC
GCTGTTGAAGACCAAGGACGGCGCCGGCCGCGTCGCCGCGCACGAGATCATGATCGGCACCCCGGCCATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCGGCAACCTGGGCATGCAGACGCTCGACCAGAAC
CTGCAGGACCTGGTGCGGCGCAACATCGTCGCTCCGGGCGTGGCGCGCGCCGCGGCGCAGAACAAAGAAGCCTTCACGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.71

99.424

0.793
  pilT Acinetobacter baumannii D1279779

79.71

99.424

0.793
  pilT Acinetobacter baumannii strain A118

79.71

99.424

0.793
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Legionella pneumophila strain Lp02

73.623

99.424

0.732
  pilT Legionella pneumophila strain ERS1305867

73.623

99.424

0.732
  pilT Neisseria meningitidis 8013

72.543

99.712

0.723
  pilT Neisseria gonorrhoeae MS11

71.965

99.712

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70

95.101

0.666
  pilT Vibrio cholerae strain A1552

70

95.101

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.727

95.101

0.501
  pilU Pseudomonas stutzeri DSM 10701

43.21

93.372

0.403
  pilU Acinetobacter baylyi ADP1

42.769

93.66

0.401
  pilU Vibrio cholerae strain A1552

40.923

93.66

0.383