Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VA613_RS12880 Genome accession   NZ_CP141769
Coordinates   2572685..2573812 (+) Length   375 a.a.
NCBI ID   WP_324779417.1    Uniprot ID   -
Organism   Thiobacillus sedimenti strain SCUT-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2567685..2578812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VA613_RS12855 (VA613_12855) - 2569110..2569421 (-) 312 WP_324779412.1 DUF167 domain-containing protein -
  VA613_RS12860 (VA613_12860) - 2569459..2570034 (-) 576 WP_324779413.1 YggT family protein -
  VA613_RS12865 (VA613_12865) proC 2570031..2570846 (-) 816 WP_324779414.1 pyrroline-5-carboxylate reductase -
  VA613_RS12870 (VA613_12870) - 2570839..2571564 (-) 726 WP_324779415.1 YggS family pyridoxal phosphate-dependent enzyme -
  VA613_RS12875 (VA613_12875) pilT 2571631..2572674 (+) 1044 WP_324779416.1 type IV pilus twitching motility protein PilT Machinery gene
  VA613_RS12880 (VA613_12880) pilU 2572685..2573812 (+) 1128 WP_324779417.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VA613_RS12885 (VA613_12885) - 2573904..2574287 (-) 384 WP_324779418.1 hypothetical protein -
  VA613_RS12890 (VA613_12890) - 2574372..2575622 (-) 1251 WP_324779419.1 THUMP domain-containing protein -
  VA613_RS12895 (VA613_12895) - 2575642..2576055 (-) 414 WP_324779420.1 CopD family protein -
  VA613_RS12900 (VA613_12900) - 2576054..2576590 (+) 537 WP_324779421.1 TlpA disulfide reductase family protein -
  VA613_RS12905 (VA613_12905) - 2576587..2576790 (+) 204 WP_324779422.1 SlyX family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41922.24 Da        Isoelectric Point: 6.4360

>NTDB_id=919317 VA613_RS12880 WP_324779417.1 2572685..2573812(+) (pilU) [Thiobacillus sedimenti strain SCUT-2]
MNAEKTVHDLLRLMLAKNASDLFITAGFPPAIKVDGKITPVSNQSLTPAHTSELARSIMNERQWKDFEATNESNFAISPP
EIGRFRVNVFVQQGRVGIVMRTINTKIPKMEELNLPPILKDVAMIKRGLVIFVGGTGTGKSTSLAAMVGYRNENDAGHII
TVEDPIEYVHEHRKSIITQREVGIDTDNWFSALKNTLRQAPDVILIGEIRDRDTMEYAIAFAETGHLCLSTLHANSANQA
LDRIINFFPEEKRDQLLMDLSLNLKAFISQRLVPRKGTNGRVAAVEILLNSPLIADLIFKGHVHEIKEIMAKSRELGMQT
FDQALFDLYEAGMISYEDALRNADSLNDLRLQIKLHGQEAKGRDMMAGLDHLDIV

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=919317 VA613_RS12880 WP_324779417.1 2572685..2573812(+) (pilU) [Thiobacillus sedimenti strain SCUT-2]
ATGAACGCCGAAAAAACCGTCCACGACCTGCTGCGCCTGATGCTGGCGAAGAACGCCTCCGACCTCTTCATCACCGCGGG
CTTCCCGCCCGCGATCAAGGTCGACGGCAAGATCACCCCGGTGTCGAACCAGAGCCTGACGCCTGCCCACACCAGCGAGC
TCGCACGTTCGATCATGAACGAGCGGCAATGGAAGGATTTCGAGGCCACCAACGAATCCAACTTCGCGATCAGCCCGCCC
GAGATCGGCCGCTTCCGCGTCAACGTCTTCGTGCAGCAGGGCCGCGTCGGCATCGTGATGCGGACGATCAACACCAAGAT
CCCGAAGATGGAGGAGCTGAACCTGCCGCCCATCCTCAAGGACGTCGCGATGATCAAGCGCGGACTGGTGATCTTCGTCG
GCGGCACCGGCACCGGTAAATCGACCTCGCTGGCGGCGATGGTGGGCTACCGCAACGAGAACGACGCCGGGCACATCATC
ACCGTCGAGGACCCGATCGAATACGTCCACGAGCACCGGAAATCGATCATCACCCAGCGCGAGGTCGGCATCGACACCGA
CAACTGGTTCTCGGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTCATCCTCATCGGCGAAATCCGCGACCGCGACA
CCATGGAATACGCCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTCGACGCTGCACGCCAACAGCGCCAACCAGGCG
CTCGACCGCATCATCAACTTCTTCCCCGAGGAAAAGCGCGACCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGCCTT
CATCTCGCAGCGCCTCGTGCCGCGCAAGGGCACCAACGGCCGCGTCGCCGCGGTCGAGATCCTGCTCAACTCGCCGCTGA
TCGCCGACCTGATCTTCAAGGGCCACGTGCACGAGATCAAGGAGATCATGGCGAAGTCGCGCGAGCTCGGCATGCAGACC
TTCGACCAGGCGCTATTCGACCTCTACGAGGCCGGCATGATTTCCTACGAGGACGCGCTACGCAACGCCGACAGCCTCAA
CGACCTGCGCCTGCAGATCAAGCTGCACGGCCAGGAAGCCAAGGGGCGCGACATGATGGCGGGACTGGATCACCTCGATA
TCGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.291

95.467

0.595
  pilU Acinetobacter baylyi ADP1

59.944

95.2

0.571
  pilU Vibrio cholerae strain A1552

56.012

90.933

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.023

91.467

0.403
  pilT Acinetobacter baylyi ADP1

43.363

90.4

0.392
  pilT Acinetobacter baumannii D1279779

43.844

88.8

0.389
  pilT Acinetobacter baumannii strain A118

43.844

88.8

0.389
  pilT Acinetobacter nosocomialis M2

43.844

88.8

0.389
  pilT Pseudomonas aeruginosa PAK

43.027

89.867

0.387
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

88.533

0.373
  pilT Vibrio cholerae strain A1552

42.169

88.533

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.867

0.373
  pilT Legionella pneumophila strain Lp02

41.742

88.8

0.371
  pilT Legionella pneumophila strain ERS1305867

41.742

88.8

0.371