Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OC193_RS13445 Genome accession   NZ_AP025476
Coordinates   3025148..3026185 (-) Length   345 a.a.
NCBI ID   WP_048659419.1    Uniprot ID   A0A4U1VD25
Organism   Vibrio crassostreae strain LMG 22240     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3020148..3031185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OC193_RS13415 rsmE 3020860..3021591 (+) 732 WP_048662976.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OC193_RS13420 gshB 3021601..3022551 (+) 951 WP_048662975.1 glutathione synthase -
  OC193_RS13425 - 3022656..3023219 (+) 564 WP_048611435.1 YqgE/AlgH family protein -
  OC193_RS13430 ruvX 3023275..3023697 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  OC193_RS13435 - 3023796..3023954 (+) 159 WP_165886710.1 hypothetical protein -
  OC193_RS13440 pilU 3024024..3025133 (-) 1110 WP_048662973.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OC193_RS13445 pilT 3025148..3026185 (-) 1038 WP_048659419.1 type IV pilus twitching motility protein PilT Machinery gene
  OC193_RS13450 - 3026212..3026916 (+) 705 WP_048662972.1 YggS family pyridoxal phosphate-dependent enzyme -
  OC193_RS13455 proC 3026993..3027811 (+) 819 WP_048662970.1 pyrroline-5-carboxylate reductase -
  OC193_RS13460 - 3027888..3028445 (+) 558 WP_048662969.1 YggT family protein -
  OC193_RS13465 yggU 3028475..3028765 (+) 291 WP_009847703.1 DUF167 family protein YggU -
  OC193_RS13470 - 3028794..3029225 (+) 432 WP_048662968.1 DUF4426 domain-containing protein -
  OC193_RS13475 - 3029272..3029871 (+) 600 WP_048662966.1 XTP/dITP diphosphatase -
  OC193_RS13480 hemW 3029871..3031058 (+) 1188 WP_048662965.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38229.76 Da        Isoelectric Point: 6.0507

>NTDB_id=91885 OC193_RS13445 WP_048659419.1 3025148..3026185(-) (pilT) [Vibrio crassostreae strain LMG 22240]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLDQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=91885 OC193_RS13445 WP_048659419.1 3025148..3026185(-) (pilT) [Vibrio crassostreae strain LMG 22240]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGCGATTCGCAGCGTGGTGAGTTTGAAGAAAAATTGGAAGTCGACTTCTCTTTTGAATTGCCCAATGTTGGTCGT
TTCCGTGTGAATGCTTTTAACCAAGCTCGTGGTTGCTCGGCTGTCTTTCGAACTATCCCTGTAGAGATCCCAACTTTAGA
TCAACTAGGTGCGCCTGAAATCTTTGAAAAGATTGCCAACTACGAAAAAGGTTTAGTGCTCGTTACTGGCCCAACAGGCT
CTGGTAAATCAACGACCCTTGCGGCGATGGTTGATTACGTAAACCGCAACCACAACAAACATATCCTGACGATCGAAGAC
CCGATTGAATTCGTTCACACCAACAATAAATGCCTTGTTAACCAGCGTGAGGTCCATCGTGATACTCACAGCTTCAAAGC
GGCGCTGCGCAGCGCGTTACGTGAAGACCCAGATGTTATCCTTGTTGGTGAGCTTCGTGACCAAGAAACGATCAGCTTAG
CGCTAACCGCAGCGGAAACCGGTCACTTGGTTTTTGGTACCCTACATACCAGCTCTGCAGCAAAAACGATTGACCGTATC
ATTGATGTTTTCCCTGGTAGCGATAAAGACATGGTGCGTTCAATGCTGTCTGAATCGTTACGATCGGTAATTGCACAGAA
GCTGCTAAAACGTGTAGGGGGGGGGCGTGTGGCTTGCCATGAAATCATGATGGCGACACCAGCGATCAGAAACTTGATTC
GTGAAGATAAGGTCGCTCAGATGTATTCGATCATTCAAACGGGCGCAGCACATGGCATGCAAACTATGGAGCAAAACGCG
AAGCAGCTGATGGCTCAAGGCTTGGTTGATTCGGAAGAGGTTGAGAAAAAAATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U1VD25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.812

0.394
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment