Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OC193_RS13440 Genome accession   NZ_AP025476
Coordinates   3024024..3025133 (-) Length   369 a.a.
NCBI ID   WP_048662973.1    Uniprot ID   -
Organism   Vibrio crassostreae strain LMG 22240     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3019024..3030133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OC193_RS13405 - 3019323..3019823 (+) 501 WP_048662979.1 SprT family zinc-dependent metalloprotease -
  OC193_RS13410 - 3019975..3020706 (+) 732 WP_048662978.1 endonuclease -
  OC193_RS13415 rsmE 3020860..3021591 (+) 732 WP_048662976.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OC193_RS13420 gshB 3021601..3022551 (+) 951 WP_048662975.1 glutathione synthase -
  OC193_RS13425 - 3022656..3023219 (+) 564 WP_048611435.1 YqgE/AlgH family protein -
  OC193_RS13430 ruvX 3023275..3023697 (+) 423 WP_048659417.1 Holliday junction resolvase RuvX -
  OC193_RS13435 - 3023796..3023954 (+) 159 WP_165886710.1 hypothetical protein -
  OC193_RS13440 pilU 3024024..3025133 (-) 1110 WP_048662973.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OC193_RS13445 pilT 3025148..3026185 (-) 1038 WP_048659419.1 type IV pilus twitching motility protein PilT Machinery gene
  OC193_RS13450 - 3026212..3026916 (+) 705 WP_048662972.1 YggS family pyridoxal phosphate-dependent enzyme -
  OC193_RS13455 proC 3026993..3027811 (+) 819 WP_048662970.1 pyrroline-5-carboxylate reductase -
  OC193_RS13460 - 3027888..3028445 (+) 558 WP_048662969.1 YggT family protein -
  OC193_RS13465 yggU 3028475..3028765 (+) 291 WP_009847703.1 DUF167 family protein YggU -
  OC193_RS13470 - 3028794..3029225 (+) 432 WP_048662968.1 DUF4426 domain-containing protein -
  OC193_RS13475 - 3029272..3029871 (+) 600 WP_048662966.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41396.33 Da        Isoelectric Point: 5.8700

>NTDB_id=91884 OC193_RS13440 WP_048662973.1 3024024..3025133(-) (pilU) [Vibrio crassostreae strain LMG 22240]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEKLNLVQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTSRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQTLY
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=91884 OC193_RS13440 WP_048662973.1 3024024..3025133(-) (pilU) [Vibrio crassostreae strain LMG 22240]
ATGGAATTGAATCAAATCCTTGAGGGAATGCTGTCTCAAAAAGCGTCCGATCTTTATATCACTGTCGATGCGCCTGTCTT
GTTCCGTGTCGATGGTGAACTACGACCGCAAGGCGAGAAGCTGAATTTGGTTCAGGTCGCTCAACTACTTGATGCGATGA
TGGATCAAGACCGACGTGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCCATAGTGCGTGACTTTGGGCGTTTTCGT
GTCAGTGCATTCTTTCAACGAGAGCTACCTGGAGCGGTCATTCGACGTATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCAGATGTACTGCAGGACCTTTCAATCGCTAAGCGCGGACTTGTGTTGGTGGTTGGGGCGACAGGCTCTGGTA
AATCGACCTCAATGGCTGCGATGACTGGTTATCGCAATACCAGCCGCTCGGGGCATATCTTGACGGTTGAAGACCCAATT
GAATTCGTTCACGAACATAAAAAATGTATCGTGACTCAACGTGAGGTTGGGCTCGACACCGAGAGCTATGAAGTCGCACT
TAAGAACTCGTTACGTCAAGCGCCAGACATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCAACTTTGCACGCCAATAACGCTAACCAAGCGTTAGAACGTATTCTTCAC
TTGGTGCCGAAAGAGCAGAAAGAGCAGTTCCTGTTTGATTTGTCGATGAACTTGCGTGGCGTGGTTGCTCAACAGTTAAT
CCGAGATAAGAATGGCAGCGGTCGTCATGGTGTGTTTGAAATTCTGCTGAATAGCCCTCGTGTGTCTGACTTGATTCGTC
GTGGTGAACTACATGAACTGAAAGCAACCATGGCAAAATCAAAAGAGATTGGTATGCAGACCTTTGACCAAACTTTATAT
GATTTAGTTGTAGCAGGCAAGATCAGCGAAGAGGATGCGTTTCACAGTGCTGACTCTGCTAATGACTTACGTTTGATGCT
AAAAACCAGACGTGGCGATGATGACTACGGGACTGGTGCTTTGGCCGGGGTAAAGATAGACATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.967

99.729

0.837
  pilU Pseudomonas stutzeri DSM 10701

55.795

100

0.561
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Legionella pneumophila strain Lp02

42.69

92.683

0.396
  pilT Legionella pneumophila strain ERS1305867

42.69

92.683

0.396
  pilT Acinetobacter baylyi ADP1

42.262

91.057

0.385
  pilT Acinetobacter baumannii strain A118

41.279

93.225

0.385
  pilT Acinetobacter baumannii D1279779

41.279

93.225

0.385
  pilT Acinetobacter nosocomialis M2

41.279

93.225

0.385
  pilT Pseudomonas aeruginosa PAK

41.399

92.954

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

87.805

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.233

92.954

0.374
  pilT Neisseria meningitidis 8013

38.682

94.58

0.366
  pilT Neisseria gonorrhoeae MS11

38.682

94.58

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366

Multiple sequence alignment