Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV39_RS02060 Genome accession   NZ_AP025472
Coordinates   425535..426572 (+) Length   345 a.a.
NCBI ID   WP_017054690.1    Uniprot ID   A0A1E5D0S2
Organism   Vibrio cortegadensis strain CECT 7227     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 420535..431572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV39_RS02025 hemW 420746..421951 (-) 1206 WP_171757187.1 radical SAM family heme chaperone HemW -
  OCV39_RS02030 - 421951..422547 (-) 597 WP_017054696.1 XTP/dITP diphosphatase -
  OCV39_RS02035 - 422586..423017 (-) 432 WP_171757185.1 DUF4426 domain-containing protein -
  OCV39_RS02040 yggU 423067..423357 (-) 291 WP_017054694.1 DUF167 family protein YggU -
  OCV39_RS02045 - 423357..423914 (-) 558 WP_017054693.1 YggT family protein -
  OCV39_RS02050 proC 423958..424776 (-) 819 WP_171757184.1 pyrroline-5-carboxylate reductase -
  OCV39_RS02055 - 424809..425510 (-) 702 WP_113795760.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV39_RS02060 pilT 425535..426572 (+) 1038 WP_017054690.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV39_RS02065 pilU 426617..427723 (+) 1107 WP_171757183.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV39_RS02070 ruvX 427835..428272 (-) 438 WP_113795762.1 Holliday junction resolvase RuvX -
  OCV39_RS02075 - 428269..428832 (-) 564 WP_017054687.1 YqgE/AlgH family protein -
  OCV39_RS02080 gshB 428891..429838 (-) 948 WP_017054686.1 glutathione synthase -
  OCV39_RS02085 rsmE 429852..430583 (-) 732 WP_017054685.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV39_RS02090 - 430721..431419 (-) 699 WP_261888837.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38458.03 Da        Isoelectric Point: 6.1486

>NTDB_id=91820 OCV39_RS02060 WP_017054690.1 425535..426572(+) (pilT) [Vibrio cortegadensis strain CECT 7227]
MDISELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFSHADVHRLVFEIMNDAQRSEYEEKLEVDFSFELPQVGR
FRVNAFNQARGCAAVFRTIPVEIPTLEQLDAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRHHNKHILTIED
PIEFVHTNNKSLVNQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMMSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNS
KQLIAQGLVELEEVQKKIEIETSLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=91820 OCV39_RS02060 WP_017054690.1 425535..426572(+) (pilT) [Vibrio cortegadensis strain CECT 7227]
ATGGATATCTCTGAATTACTGGATTTTAGTGTAAAGCATAACGCATCAGATCTACATCTTTCTGCAGGTGTTCCTCCTAT
GGTACGTATAGATGGCGAAGTAAGAAAGCTTGGAGTGCCAGCTTTTAGCCATGCAGATGTACATCGTTTGGTTTTTGAGA
TCATGAACGATGCTCAACGTAGCGAGTATGAAGAAAAACTGGAGGTCGATTTCTCATTTGAACTTCCTCAAGTTGGACGT
TTTCGTGTGAATGCTTTCAATCAAGCACGCGGCTGCGCAGCTGTATTTCGTACTATTCCAGTGGAGATCCCAACATTAGA
GCAGTTGGATGCACCGGAAATATTTGAAAAAATAGCTAATTATGAGAAAGGTTTAGTTTTGGTTACTGGGCCTACTGGTT
CTGGTAAATCGACAACCTTGGCGGCCATGGTCGATTATGTGAATCGTCACCATAATAAGCATATCTTAACCATTGAAGAT
CCGATCGAGTTCGTTCACACCAATAACAAAAGCTTAGTGAATCAGCGTGAAGTCCATCGTGATACTCACAGTTTTAAAAA
TGCTTTACGTTCAGCTTTGCGTGAAGACCCTGATGTGATCTTAGTGGGGGAGTTGCGAGACCAAGAGACGATCAGCTTAG
CGTTAACCGCAGCCGAAACAGGACACTTAGTTTTTGGTACGTTGCACACCAGCTCTGCGGCGAAAACTATTGACCGTATT
ATTGATGTCTTCCCAGGTAGCGATAAAGATATGGTTCGTTCGATGATGTCTGAGTCATTACGTGCGGTGATTGCTCAAAA
ACTCTTAAAACGCATTGGTGGTGGGCGAGTTGCGTGTCATGAAATTATGATGGCAACCCCTGCAATTCGTAACTTGATCC
GTGAAGATAAAGTCGCGCAAATGTACTCCATCATTCAAACCGGCGCCGCTCATGGCATGCAAACCATGGAGCAAAATTCG
AAACAGCTCATTGCTCAAGGTTTGGTTGAGTTGGAAGAAGTGCAGAAAAAAATTGAAATAGAAACCTCACTGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E5D0S2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

91.014

100

0.91
  pilT Vibrio cholerae strain A1552

91.014

100

0.91
  pilT Acinetobacter baumannii D1279779

72.036

95.362

0.687
  pilT Acinetobacter baumannii strain A118

72.036

95.362

0.687
  pilT Acinetobacter nosocomialis M2

71.733

95.362

0.684
  pilT Acinetobacter baylyi ADP1

71.299

95.942

0.684
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.941

98.551

0.67
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

63.188

100

0.632
  pilT Neisseria gonorrhoeae MS11

62.899

100

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.201

92.174

0.481
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.812

0.394
  pilU Acinetobacter baylyi ADP1

39.062

92.754

0.362

Multiple sequence alignment