Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV39_RS02065 Genome accession   NZ_AP025472
Coordinates   426617..427723 (+) Length   368 a.a.
NCBI ID   WP_171757183.1    Uniprot ID   -
Organism   Vibrio cortegadensis strain CECT 7227     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421617..432723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV39_RS02030 - 421951..422547 (-) 597 WP_017054696.1 XTP/dITP diphosphatase -
  OCV39_RS02035 - 422586..423017 (-) 432 WP_171757185.1 DUF4426 domain-containing protein -
  OCV39_RS02040 yggU 423067..423357 (-) 291 WP_017054694.1 DUF167 family protein YggU -
  OCV39_RS02045 - 423357..423914 (-) 558 WP_017054693.1 YggT family protein -
  OCV39_RS02050 proC 423958..424776 (-) 819 WP_171757184.1 pyrroline-5-carboxylate reductase -
  OCV39_RS02055 - 424809..425510 (-) 702 WP_113795760.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV39_RS02060 pilT 425535..426572 (+) 1038 WP_017054690.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV39_RS02065 pilU 426617..427723 (+) 1107 WP_171757183.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV39_RS02070 ruvX 427835..428272 (-) 438 WP_113795762.1 Holliday junction resolvase RuvX -
  OCV39_RS02075 - 428269..428832 (-) 564 WP_017054687.1 YqgE/AlgH family protein -
  OCV39_RS02080 gshB 428891..429838 (-) 948 WP_017054686.1 glutathione synthase -
  OCV39_RS02085 rsmE 429852..430583 (-) 732 WP_017054685.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV39_RS02090 - 430721..431419 (-) 699 WP_261888837.1 endonuclease -
  OCV39_RS02095 - 431553..432065 (-) 513 WP_017054683.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41351.25 Da        Isoelectric Point: 5.6702

>NTDB_id=91821 OCV39_RS02065 WP_171757183.1 426617..427723(+) (pilU) [Vibrio cortegadensis strain CECT 7227]
MELNQILAGMNEEKASDLYITVGAPILFRIDGELRPQGEKLSEDQVTSLLDAMMDADRQNEFRQSRESNFAIMRDFGRFR
VSAFFQRELPGAVIRRIETVIPTFEQLKLPVVLQDLAIAKRGLVLVVGATGSGKSTSMAAMTGYRNSNRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRNRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGIVAQQLIRDKNGKGRHGVFEILLNSPRISDLIRRGDLHELKATMAKSQEIGMQTFDQSLY
KLVVEGKINEEDALHSADSANDLRLMLKTQSKTDSDSSSLSGIKIEMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=91821 OCV39_RS02065 WP_171757183.1 426617..427723(+) (pilU) [Vibrio cortegadensis strain CECT 7227]
ATGGAATTAAATCAAATCCTCGCGGGGATGAATGAAGAAAAAGCATCGGATCTTTATATTACTGTCGGTGCTCCTATTCT
CTTTCGTATTGATGGGGAGCTAAGACCACAAGGTGAAAAGCTATCAGAAGATCAGGTAACAAGCTTGCTTGATGCGATGA
TGGATGCCGATCGTCAGAATGAGTTTCGCCAATCCCGTGAATCAAATTTTGCGATTATGAGAGATTTTGGGCGTTTTCGT
GTGAGTGCTTTTTTCCAGCGAGAACTGCCAGGCGCGGTGATTCGCCGTATTGAAACGGTGATCCCGACGTTTGAGCAATT
GAAATTGCCCGTGGTATTACAAGACCTTGCGATTGCAAAACGTGGATTAGTGCTGGTGGTTGGTGCGACTGGTTCGGGTA
AATCGACATCAATGGCCGCGATGACAGGCTATCGCAATAGCAATCGGTCGGGTCATATTTTGACGGTAGAAGATCCGATT
GAATTCGTGCATGAACATCAGCGTTGTATTGTGACCCAGCGTGAAGTCGGTTTAGACACGGATAGCTATGAAGTGGCGTT
GAAAAACTCACTGCGTCAGGCTCCAGATATGATCTTAATTGGTGAAATTCGTAACCGAGAAACCATGGAATACGCGATGA
CGTTTGCTGAAACCGGGCATCTTTGTATGGCGACTCTGCACGCGAATAACGCTAACCAAGCCTTAGAACGCATTTTGCAC
TTAGTACCGAAAGAGCAAAAAGAGCAGTTCCTATTCGATCTTTCTATGAACTTAAAAGGCATTGTGGCTCAGCAATTGAT
TCGTGATAAAAACGGAAAAGGTCGTCATGGGGTATTTGAGATCTTGCTTAATAGTCCAAGGATCTCAGATTTGATCCGTC
GAGGCGATCTACATGAATTAAAAGCGACAATGGCGAAATCTCAAGAGATTGGAATGCAAACCTTTGATCAATCACTGTAT
AAGTTAGTCGTGGAAGGGAAGATCAATGAGGAAGATGCACTTCATAGTGCTGATTCAGCGAACGATCTACGTTTAATGCT
GAAAACACAGTCCAAAACAGATTCTGACAGTAGCAGCTTAAGTGGCATAAAAATCGAAATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58.491

100

0.59
  pilU Acinetobacter baylyi ADP1

53.561

95.38

0.511
  pilT Legionella pneumophila strain ERS1305867

42.69

92.935

0.397
  pilT Legionella pneumophila strain Lp02

42.69

92.935

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

92.12

0.389
  pilT Acinetobacter baylyi ADP1

40.176

92.663

0.372
  pilT Pseudomonas aeruginosa PAK

40.118

92.12

0.37
  pilT Acinetobacter baumannii strain A118

40.118

92.12

0.37
  pilT Acinetobacter nosocomialis M2

40.118

92.12

0.37
  pilT Acinetobacter baumannii D1279779

40.118

92.12

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.761

0.364
  pilT Vibrio cholerae strain A1552

40.12

90.761

0.364

Multiple sequence alignment