Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV20_RS02275 Genome accession   NZ_AP025470
Coordinates   445205..446314 (+) Length   369 a.a.
NCBI ID   WP_017062241.1    Uniprot ID   -
Organism   Vibrio coralliirubri strain DSM 27495     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 436728..447672 445205..446314 within 0


Gene organization within MGE regions


Location: 436728..447672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV20_RS02215 - 436728..437651 (+) 924 WP_086774734.1 hypothetical protein -
  OCV20_RS02220 - 437648..438298 (+) 651 WP_086774735.1 hypothetical protein -
  OCV20_RS02225 - 438298..438981 (+) 684 WP_086774736.1 hypothetical protein -
  OCV20_RS02230 - 439055..439483 (+) 429 WP_086774747.1 M15 family metallopeptidase -
  OCV20_RS02235 - 439480..439890 (+) 411 WP_086774737.1 hypothetical protein -
  OCV20_RS02240 - 439894..440052 (+) 159 WP_261881421.1 hypothetical protein -
  OCV20_RS02245 - 440361..441068 (+) 708 WP_086774738.1 SDR family NAD(P)-dependent oxidoreductase -
  OCV20_RS02250 yggU 441571..441861 (-) 291 WP_009847703.1 DUF167 family protein YggU -
  OCV20_RS02255 - 441891..442448 (-) 558 WP_017067798.1 YggT family protein -
  OCV20_RS02260 proC 442525..443343 (-) 819 WP_086774739.1 pyrroline-5-carboxylate reductase -
  OCV20_RS02265 - 443422..444126 (-) 705 WP_086774740.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV20_RS02270 pilT 444153..445190 (+) 1038 WP_086774741.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV20_RS02275 pilU 445205..446314 (+) 1110 WP_017062241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV20_RS02280 - 446389..446526 (-) 138 WP_202910134.1 hypothetical protein -
  OCV20_RS02285 ruvX 446627..447049 (-) 423 WP_081230758.1 Holliday junction resolvase RuvX -
  OCV20_RS02290 - 447109..447672 (-) 564 WP_048611435.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41365.27 Da        Isoelectric Point: 5.8713

>NTDB_id=91776 OCV20_RS02275 WP_017062241.1 445205..446314(+) (pilU) [Vibrio coralliirubri strain DSM 27495]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMEQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVIAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=91776 OCV20_RS02275 WP_017062241.1 445205..446314(+) (pilU) [Vibrio coralliirubri strain DSM 27495]
ATGGAATTGAATCAAATTCTTGAGGGGATGCTTTCACAAAAGGCGTCGGATCTTTATATCACGGTCGATGCGCCAATCCT
GTTTCGTGTGGATGGTGAATTACGCCCCCAAGGAGAGAAGCTGAATGCGGCTCAGGTTGCTCAATTACTTGATGCGATGA
TGGAACAAGATCGACGTGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCAATCGTGCGAGACTTTGGTCGTTTTCGT
GTCAGTGCATTCTTTCAGCGAGAGCTACCTGGAGCGGTCATTCGACGAATCGAGACTAATATTCCAACCTTTGAGCAACT
AAAGCTTCCTGATGTACTACAGGACCTATCAATTGCTAAGCGCGGACTTGTGCTGGTGGTTGGTGCGACTGGCTCTGGTA
AATCGACCTCAATGGCTGCAATGACAGGCTATCGCAACACCAATCGCTCGGGACATATCTTGACGGTTGAAGACCCTATT
GAATTTGTGCACGAACATAAAAAGTGCATCGTGACGCAACGTGAGGTCGGGCTCGACACCGAGAGCTATGAAGTCGCGCT
TAAGAACTCGCTACGCCAAGCTCCAGATATGATTTTGATTGGTGAAATTCGTAGTCGTGAAACCATGGAATACGCGATGA
CTTTTGCTGAAACGGGTCACTTGTGTATGGCAACCTTGCACGCCAATAACGCTAACCAAGCGCTAGAGCGTATTCTTCAT
TTGGTGCCGAAAGAACAGAAAGAGCAGTTCCTGTTCGATCTGTCGATGAACCTGCGCGGTGTTGTCGCTCAGCAGCTCAT
TCGCGATAAGAATGGCAGCGGGCGTCATGGTGTATTCGAGATTCTGCTGAATAGCCCTCGTGTGTCTGACTTGATACGTC
GTGGTGAACTACACGAACTGAAAGCGACCATGGCTAAATCGAAAGAGATAGGTATGCAGACTTTTGACCAAGCTTTGTAT
GATTTAGTGATAGCAGGCAAGATCAGCGAAGAGGACGCGTTCCACAGTGCCGATTCTGCTAATGACTTGCGTTTAATGCT
CAAAACTAGACGTGGTGATGATGACTACGGAACTGGTGCGTTGGCTGGCGTTAAGATTGATATGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

99.729

0.84
  pilU Pseudomonas stutzeri DSM 10701

56.065

100

0.564
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Acinetobacter baumannii strain A118

41.298

91.87

0.379
  pilT Acinetobacter baumannii D1279779

41.298

91.87

0.379
  pilT Acinetobacter nosocomialis M2

41.298

91.87

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Acinetobacter baylyi ADP1

41.088

89.702

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363

Multiple sequence alignment