Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV20_RS02270 Genome accession   NZ_AP025470
Coordinates   444153..445190 (+) Length   345 a.a.
NCBI ID   WP_086774741.1    Uniprot ID   -
Organism   Vibrio coralliirubri strain DSM 27495     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 436728..447672 444153..445190 within 0


Gene organization within MGE regions


Location: 436728..447672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV20_RS02215 - 436728..437651 (+) 924 WP_086774734.1 hypothetical protein -
  OCV20_RS02220 - 437648..438298 (+) 651 WP_086774735.1 hypothetical protein -
  OCV20_RS02225 - 438298..438981 (+) 684 WP_086774736.1 hypothetical protein -
  OCV20_RS02230 - 439055..439483 (+) 429 WP_086774747.1 M15 family metallopeptidase -
  OCV20_RS02235 - 439480..439890 (+) 411 WP_086774737.1 hypothetical protein -
  OCV20_RS02240 - 439894..440052 (+) 159 WP_261881421.1 hypothetical protein -
  OCV20_RS02245 - 440361..441068 (+) 708 WP_086774738.1 SDR family NAD(P)-dependent oxidoreductase -
  OCV20_RS02250 yggU 441571..441861 (-) 291 WP_009847703.1 DUF167 family protein YggU -
  OCV20_RS02255 - 441891..442448 (-) 558 WP_017067798.1 YggT family protein -
  OCV20_RS02260 proC 442525..443343 (-) 819 WP_086774739.1 pyrroline-5-carboxylate reductase -
  OCV20_RS02265 - 443422..444126 (-) 705 WP_086774740.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV20_RS02270 pilT 444153..445190 (+) 1038 WP_086774741.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV20_RS02275 pilU 445205..446314 (+) 1110 WP_017062241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV20_RS02280 - 446389..446526 (-) 138 WP_202910134.1 hypothetical protein -
  OCV20_RS02285 ruvX 446627..447049 (-) 423 WP_081230758.1 Holliday junction resolvase RuvX -
  OCV20_RS02290 - 447109..447672 (-) 564 WP_048611435.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38275.81 Da        Isoelectric Point: 6.0507

>NTDB_id=91775 OCV20_RS02270 WP_086774741.1 444153..445190(+) (pilT) [Vibrio coralliirubri strain DSM 27495]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLDQLGAPEIFERIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGMVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=91775 OCV20_RS02270 WP_086774741.1 444153..445190(+) (pilT) [Vibrio coralliirubri strain DSM 27495]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTTCATCGTTTGGTTTTTGAGA
TCATGAGCGATTCACAGCGCGGCGAGTTTGAAGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTCCGTGTTAATGCGTTTAACCAAGCTCGTGGCTGCTCTGCTGTCTTTCGAACTATTCCTGTCGAAATCCCGACTTTAGA
TCAGTTAGGTGCACCTGAGATCTTCGAAAGGATTGCTAACTACGAAAAAGGCTTAGTGCTCGTTACTGGTCCAACTGGCT
CAGGTAAATCAACAACTCTTGCGGCTATGGTTGATTACGTAAATCGCAACCACAATAAGCATATTCTGACGATTGAAGAC
CCGATTGAGTTCGTTCACACCAACAATAAATGCCTAGTCAACCAGCGTGAAGTTCACCGTGATACTCATAGCTTTAAAGC
GGCGTTGCGCAGTGCGTTACGTGAAGATCCAGACGTGATTCTGGTTGGTGAGCTTCGTGACCAAGAGACGATCAGTTTAG
CGCTAACCGCAGCAGAGACCGGTCATTTAGTGTTTGGTACTTTGCATACTAGTTCTGCGGCTAAAACGATCGACCGTATT
ATCGATGTGTTCCCTGGTAGCGATAAAGATATGGTTCGTTCGATGTTGTCTGAATCACTGCGCTCGGTGATAGCCCAGAA
GCTGCTAAAGCGTGTTGGTGGTGGTCGTGTGGCGTGTCATGAAATCATGATGGCAACACCTGCGATCAGAAACTTGATTC
GTGAAGATAAGGTTGCGCAGATGTACTCGATCATTCAAACGGGCGCAGCGCATGGTATGCAGACCATGGAGCAAAACGCG
AAACAACTGATGGCGCAGGGCATGGTTGATTCGGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.029

100

0.62
  pilT Neisseria gonorrhoeae MS11

61.739

100

0.617
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.516

92.174

0.484
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.812

0.391
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment