Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV52_RS13490 Genome accession   NZ_AP025465
Coordinates   2952568..2953605 (-) Length   345 a.a.
NCBI ID   WP_137408533.1    Uniprot ID   A0A7V7TF73
Organism   Vibrio chagasii strain LMG 21353     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2947568..2958605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV52_RS13460 rsmE 2948269..2949000 (+) 732 WP_137408535.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV52_RS13465 gshB 2949010..2949960 (+) 951 WP_102424750.1 glutathione synthase -
  OCV52_RS13470 - 2950069..2950632 (+) 564 WP_102424749.1 YqgE/AlgH family protein -
  OCV52_RS13475 ruvX 2950693..2951115 (+) 423 WP_004738973.1 Holliday junction resolvase RuvX -
  OCV52_RS13480 - 2951214..2951384 (+) 171 WP_155647308.1 hypothetical protein -
  OCV52_RS13485 pilU 2951444..2952553 (-) 1110 WP_137408534.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV52_RS13490 pilT 2952568..2953605 (-) 1038 WP_137408533.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV52_RS13495 - 2953632..2954336 (+) 705 WP_061032414.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV52_RS13500 proC 2954417..2955235 (+) 819 WP_102426486.1 pyrroline-5-carboxylate reductase -
  OCV52_RS13505 - 2955312..2955869 (+) 558 WP_017069592.1 YggT family protein -
  OCV52_RS13510 yggU 2955899..2956189 (+) 291 WP_137408532.1 DUF167 family protein YggU -
  OCV52_RS13515 - 2956218..2956649 (+) 432 WP_137408531.1 DUF4426 domain-containing protein -
  OCV52_RS13520 - 2956698..2957297 (+) 600 WP_137408530.1 XTP/dITP diphosphatase -
  OCV52_RS13525 hemW 2957297..2958484 (+) 1188 WP_137408529.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38144.65 Da        Isoelectric Point: 5.8996

>NTDB_id=91749 OCV52_RS13490 WP_137408533.1 2952568..2953605(-) (pilT) [Vibrio chagasii strain LMG 21353]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLEQLGAPDIFEKIANFEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHSDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=91749 OCV52_RS13490 WP_137408533.1 2952568..2953605(-) (pilT) [Vibrio chagasii strain LMG 21353]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCACCGTTTGGTTTTTGAGA
TCATGAGCGACTCGCAGCGCGGTGAGTTCGAGGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTCGGTCGT
TTCCGTGTTAACGCTTTTAACCAAGCCCGTGGCTGCTCAGCTGTCTTTCGAACTATCCCTGTCGAGATACCAACGTTAGA
GCAGTTAGGCGCTCCTGATATCTTTGAAAAGATTGCTAATTTCGAAAAGGGTCTAGTGTTAGTTACAGGCCCAACAGGTT
CTGGTAAATCGACTACTCTTGCGGCGATGGTGGATTACGTTAACCGTAACCACAATAAGCATATTCTCACCATAGAAGAC
CCGATTGAATTTGTTCACACCAACAATAAGTGCCTAGTGAACCAGCGTGAGGTTCATAGTGATACTCATAGCTTTAAAGC
GGCGCTGCGCAGTGCGTTACGTGAAGACCCTGACGTTATCCTTGTTGGTGAACTTCGTGACCAAGAAACCATTAGTTTAG
CGCTCACGGCTGCAGAAACTGGTCACTTGGTTTTTGGTACCTTACATACCAGTTCTGCGGCAAAAACGATTGACCGTATT
ATCGATGTATTCCCGGGGAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCATTACGCTCGGTGATTGCCCAGAA
ACTATTAAAGCGTGTCGGTGGTGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCGGCGATCAGAAACTTGATTC
GTGAAGATAAAGTCGCTCAGATGTATTCAATCATTCAAACAGGCGCAGCACATGGCATGCAAACCATGGAGCAGAATGCG
AAGCAGCTGATGGCCCAAGGTTTGGTTGACTCGGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7V7TF73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.406

100

0.884
  pilT Vibrio cholerae strain A1552

88.406

100

0.884
  pilT Acinetobacter baumannii D1279779

71.687

96.232

0.69
  pilT Acinetobacter baumannii strain A118

71.687

96.232

0.69
  pilT Acinetobacter nosocomialis M2

71.386

96.232

0.687
  pilT Acinetobacter baylyi ADP1

70.606

95.652

0.675
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

66.087

100

0.661
  pilT Legionella pneumophila strain ERS1305867

66.087

100

0.661
  pilT Neisseria meningitidis 8013

62.899

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.609

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.44

98.841

0.499
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment