Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV52_RS13485 Genome accession   NZ_AP025465
Coordinates   2951444..2952553 (-) Length   369 a.a.
NCBI ID   WP_137408534.1    Uniprot ID   A0A7V7NRC8
Organism   Vibrio chagasii strain LMG 21353     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2946444..2957553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV52_RS13450 - 2946733..2947233 (+) 501 WP_102424753.1 SprT family zinc-dependent metalloprotease -
  OCV52_RS13455 - 2947384..2948115 (+) 732 WP_102424752.1 endonuclease -
  OCV52_RS13460 rsmE 2948269..2949000 (+) 732 WP_137408535.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV52_RS13465 gshB 2949010..2949960 (+) 951 WP_102424750.1 glutathione synthase -
  OCV52_RS13470 - 2950069..2950632 (+) 564 WP_102424749.1 YqgE/AlgH family protein -
  OCV52_RS13475 ruvX 2950693..2951115 (+) 423 WP_004738973.1 Holliday junction resolvase RuvX -
  OCV52_RS13480 - 2951214..2951384 (+) 171 WP_155647308.1 hypothetical protein -
  OCV52_RS13485 pilU 2951444..2952553 (-) 1110 WP_137408534.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV52_RS13490 pilT 2952568..2953605 (-) 1038 WP_137408533.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV52_RS13495 - 2953632..2954336 (+) 705 WP_061032414.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV52_RS13500 proC 2954417..2955235 (+) 819 WP_102426486.1 pyrroline-5-carboxylate reductase -
  OCV52_RS13505 - 2955312..2955869 (+) 558 WP_017069592.1 YggT family protein -
  OCV52_RS13510 yggU 2955899..2956189 (+) 291 WP_137408532.1 DUF167 family protein YggU -
  OCV52_RS13515 - 2956218..2956649 (+) 432 WP_137408531.1 DUF4426 domain-containing protein -
  OCV52_RS13520 - 2956698..2957297 (+) 600 WP_137408530.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41334.17 Da        Isoelectric Point: 5.8352

>NTDB_id=91748 OCV52_RS13485 WP_137408534.1 2951444..2952553(-) (pilU) [Vibrio chagasii strain LMG 21353]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEKLNSAQVAQLLDAMMDQERRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRHIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEVGMQTFDQALF
DLVVAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALSGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=91748 OCV52_RS13485 WP_137408534.1 2951444..2952553(-) (pilU) [Vibrio chagasii strain LMG 21353]
ATGGAATTGAATCAAATCCTTGAGGGAATGCTTTCTCAAAAAGCGTCCGATCTTTACATCACGGTTGATGCGCCAGTCTT
GTTCCGTGTCGATGGTGAGCTGCGACCGCAAGGCGAGAAACTGAATTCGGCTCAGGTCGCTCAATTACTTGATGCGATGA
TGGATCAAGAACGACGTGATGAATATCAGCAAACGCGTGAGGCTAACTTTGCCATTGTACGTGATTTTGGTCGCTTTCGT
GTTAGTGCGTTCTTTCAGCGAGAGCTACCTGGAGCAGTGATTCGACACATCGAGACGAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTATTACAAGACCTCTCAATTGCTAAGCGTGGGCTTGTGCTGGTGGTTGGGGCTACGGGTTCTGGTA
AATCGACCTCGATGGCGGCGATGACAGGCTACCGCAATACCAATCGTTCTGGGCATATCTTGACGGTTGAAGACCCGATA
GAATTCGTCCACGAACATAAGAAATGCATAGTTACTCAGCGTGAGGTAGGGCTCGACACCGAGAGCTATGAAGTCGCCCT
TAAAAACTCGTTGCGCCAAGCGCCAGATATGATTTTGATTGGCGAAATCCGTAGCCGCGAAACCATGGAATATGCGATGA
CCTTTGCTGAGACGGGTCACTTGTGTATGGCAACTTTGCACGCGAATAATGCCAACCAAGCGTTAGAGCGTATTCTTCAT
TTGGTGCCTAAAGAACAGAAAGAACAGTTCCTGTTTGATCTGTCGATGAATCTGCGTGGTGTGATTGCTCAGCAGTTAAT
TCGAGATAAGAATGGCAGCGGGCGTCATGGTGTATTTGAAATTCTACTCAACAGCCCACGAGTGTCTGACTTGATTCGTC
GCGGTGAATTGCACGAGCTAAAAGCAACAATGGCAAAATCAAAAGAAGTCGGCATGCAGACCTTTGACCAAGCTTTGTTT
GATTTAGTTGTAGCGGGAAAGATTAGCGAAGAAGATGCGTTTCATAGTGCCGATTCGGCTAATGACTTACGTTTGATGCT
AAAAACCAGACGAGGTGATGATGACTATGGAACTGGAGCATTATCTGGCGTGAAAATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7V7NRC8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.511

99.729

0.843
  pilU Pseudomonas stutzeri DSM 10701

55.795

100

0.561
  pilU Acinetobacter baylyi ADP1

54.701

95.122

0.52
  pilT Acinetobacter baumannii strain A118

42.647

92.141

0.393
  pilT Acinetobacter baumannii D1279779

42.647

92.141

0.393
  pilT Acinetobacter nosocomialis M2

42.647

92.141

0.393
  pilT Acinetobacter baylyi ADP1

42.105

92.683

0.39
  pilT Pseudomonas aeruginosa PAK

41.888

91.87

0.385
  pilT Legionella pneumophila strain ERS1305867

41.888

91.87

0.385
  pilT Legionella pneumophila strain Lp02

41.888

91.87

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

87.805

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.413

91.87

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.515

0.369
  pilT Vibrio cholerae strain A1552

40.719

90.515

0.369
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363

Multiple sequence alignment