Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV19_RS02200 Genome accession   NZ_AP025463
Coordinates   441074..442183 (+) Length   369 a.a.
NCBI ID   WP_065675458.1    Uniprot ID   A0A1C3J9P6
Organism   Vibrio celticus strain CECT 7224     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436074..447183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV19_RS02165 - 436337..436936 (-) 600 WP_065675454.1 XTP/dITP diphosphatase -
  OCV19_RS02170 - 436985..437416 (-) 432 WP_065675455.1 DUF4426 domain-containing protein -
  OCV19_RS02175 yggU 437445..437735 (-) 291 WP_009847703.1 DUF167 family protein YggU -
  OCV19_RS02180 - 437765..438322 (-) 558 WP_017067798.1 YggT family protein -
  OCV19_RS02185 proC 438399..439217 (-) 819 WP_065675456.1 pyrroline-5-carboxylate reductase -
  OCV19_RS02190 - 439291..439995 (-) 705 WP_065675457.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV19_RS02195 pilT 440022..441059 (+) 1038 WP_017062242.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV19_RS02200 pilU 441074..442183 (+) 1110 WP_065675458.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV19_RS02205 - 442258..442395 (-) 138 WP_167352278.1 hypothetical protein -
  OCV19_RS02210 ruvX 442495..442917 (-) 423 WP_065675459.1 Holliday junction resolvase RuvX -
  OCV19_RS02215 - 442978..443541 (-) 564 WP_048611435.1 YqgE/AlgH family protein -
  OCV19_RS02220 gshB 443646..444596 (-) 951 WP_065675460.1 glutathione synthase -
  OCV19_RS02225 rsmE 444606..445337 (-) 732 WP_048615674.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV19_RS02230 - 445491..446222 (-) 732 WP_065675461.1 endonuclease -
  OCV19_RS02235 - 446319..446819 (-) 501 WP_048605835.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41508.48 Da        Isoelectric Point: 5.8729

>NTDB_id=91687 OCV19_RS02200 WP_065675458.1 441074..442183(+) (pilU) [Vibrio celticus strain CECT 7224]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMEQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGCGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVIAGKIGEEDAFHSADSANDLRLMLKTRRGDDDYGTGALADVKIDMR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=91687 OCV19_RS02200 WP_065675458.1 441074..442183(+) (pilU) [Vibrio celticus strain CECT 7224]
ATGGAATTGAATCAAATCCTTGAGGGAATGCTTTCTCAAAAGGCATCGGATCTTTATATCACTGTCGATGCACCAATCCT
GTTTCGTGTGGATGGTGAATTACGCCCCCAAGGAGAGAAGTTGAATGCGGCTCAGGTTGCTCAATTACTTGATGCGATGA
TGGAACAAGACCGACGTGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCGATTGTGCGTGACTTTGGTCGTTTTCGT
GTCAGTGCATTCTTTCAGCGAGAGCTGCCTGGAGCGGTCATTCGACGTATCGAGACTAACATCCCAACCTTTGAGCAACT
AAAGCTTCCTGATGTACTGCAAGACCTGTCAATTGCTAAGCGCGGACTTGTGCTGGTGGTTGGGGCGACTGGCTCTGGTA
AATCGACCTCAATGGCTGCAATGACAGGCTATCGTAACACCAATCGCTCGGGGCATATTTTGACGGTTGAAGACCCGATT
GAATTCGTACATGAGCACAAAAAGTGTATCGTGACTCAGCGTGAAGTAGGGCTGGATACCGAGAGCTATGAAGTCGCACT
TAAGAACTCGCTACGCCAAGCACCAGATATGATTTTGATTGGTGAAATTCGTAGCCGTGAAACCATGGAGTACGCGATGA
CTTTTGCTGAGACGGGGCATTTGTGTATGGCAACCTTGCACGCGAATAATGCTAACCAAGCGTTAGAGCGTATTCTTCAC
TTGGTGCCGAAAGAACAGAAAGAACAGTTTTTGTTTGATTTGTCGATGAACCTACGTGGTGTCGTCGCTCAGCAACTTAT
TCGCGACAAGAATGGTTGCGGGCGTCATGGTGTATTCGAGATTCTGCTGAATAGCCCTCGTGTGTCTGACTTGATTCGTC
GTGGTGAACTGCACGAACTGAAAGCAACCATGGCTAAATCGAAAGAAATTGGTATGCAGACTTTTGACCAAGCTTTGTAT
GATTTAGTGATAGCAGGCAAGATCGGTGAAGAGGACGCGTTCCACAGTGCCGATTCTGCTAATGACTTGCGCTTAATGTT
AAAGACTAGACGTGGTGATGATGACTATGGAACTGGGGCTTTGGCTGATGTGAAGATAGATATGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3J9P6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.967

99.729

0.837
  pilU Pseudomonas stutzeri DSM 10701

58.286

94.851

0.553
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Legionella pneumophila strain ERS1305867

41.888

91.87

0.385
  pilT Legionella pneumophila strain Lp02

41.888

91.87

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas aeruginosa PAK

41.493

90.786

0.377
  pilT Acinetobacter baumannii D1279779

41.003

91.87

0.377
  pilT Acinetobacter baumannii strain A118

41.003

91.87

0.377
  pilT Acinetobacter nosocomialis M2

41.003

91.87

0.377
  pilT Acinetobacter baylyi ADP1

41.212

89.431

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363
  pilT Pseudomonas stutzeri DSM 10701

40

90.786

0.363

Multiple sequence alignment