Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV19_RS02195 Genome accession   NZ_AP025463
Coordinates   440022..441059 (+) Length   345 a.a.
NCBI ID   WP_017062242.1    Uniprot ID   A0A4U2ACB1
Organism   Vibrio celticus strain CECT 7224     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 435022..446059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV19_RS02160 hemW 435150..436337 (-) 1188 WP_065675453.1 radical SAM family heme chaperone HemW -
  OCV19_RS02165 - 436337..436936 (-) 600 WP_065675454.1 XTP/dITP diphosphatase -
  OCV19_RS02170 - 436985..437416 (-) 432 WP_065675455.1 DUF4426 domain-containing protein -
  OCV19_RS02175 yggU 437445..437735 (-) 291 WP_009847703.1 DUF167 family protein YggU -
  OCV19_RS02180 - 437765..438322 (-) 558 WP_017067798.1 YggT family protein -
  OCV19_RS02185 proC 438399..439217 (-) 819 WP_065675456.1 pyrroline-5-carboxylate reductase -
  OCV19_RS02190 - 439291..439995 (-) 705 WP_065675457.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV19_RS02195 pilT 440022..441059 (+) 1038 WP_017062242.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV19_RS02200 pilU 441074..442183 (+) 1110 WP_065675458.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV19_RS02205 - 442258..442395 (-) 138 WP_167352278.1 hypothetical protein -
  OCV19_RS02210 ruvX 442495..442917 (-) 423 WP_065675459.1 Holliday junction resolvase RuvX -
  OCV19_RS02215 - 442978..443541 (-) 564 WP_048611435.1 YqgE/AlgH family protein -
  OCV19_RS02220 gshB 443646..444596 (-) 951 WP_065675460.1 glutathione synthase -
  OCV19_RS02225 rsmE 444606..445337 (-) 732 WP_048615674.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38257.78 Da        Isoelectric Point: 6.0507

>NTDB_id=91686 OCV19_RS02195 WP_017062242.1 440022..441059(+) (pilT) [Vibrio celticus strain CECT 7224]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLDQLGAPEIFERIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=91686 OCV19_RS02195 WP_017062242.1 440022..441059(+) (pilT) [Vibrio celticus strain CECT 7224]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGCGATTCACAGCGCGGCGAGTTTGAAGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTCCGTGTTAATGCGTTTAACCAAGCTCGTGGCTGCTCTGCTGTCTTTCGAACTATTCCTGTCGAAATCCCGACTTTAGA
TCAGTTAGGTGCACCTGAGATCTTCGAAAGGATTGCTAACTACGAAAAAGGCTTAGTGCTCGTTACTGGTCCAACTGGCT
CTGGTAAATCAACGACTCTTGCAGCGATGGTTGATTACGTAAATCGCAACCACAATAAGCATATCCTGACGATTGAAGAC
CCGATTGAATTCGTTCACACCAATAATAAATGCTTAGTCAACCAGCGTGAAGTTCACCGTGATACTCACAGTTTTAAAGC
GGCACTGCGCAGTGCGTTACGTGAAGACCCGGACGTGATTCTTGTTGGTGAGCTTCGTGACCAAGAGACGATTAGCTTAG
CGTTAACCGCAGCAGAAACGGGTCATTTAGTGTTTGGTACTCTGCATACTAGTTCTGCGGCTAAAACGATCGACCGTATT
ATCGATGTGTTCCCAGGTAGCGATAAAGATATGGTTCGTTCGATGTTGTCTGAATCACTGCGCTCGGTGATAGCCCAGAA
GCTGCTAAAGCGTGTTGGTGGTGGTCGTGTGGCGTGTCATGAAATCATGATGGCGACACCTGCGATCAGAAACTTGATTC
GTGAAGACAAGGTTGCGCAGATGTATTCGATCATTCAAACGGGCGCAGCGCACGGCATGCAGACCATGGAACAAAATGCG
AAGCAGCTGATGGCGCAGGGCTTGGTTGATTCGGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U2ACB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Pseudomonas aeruginosa PAK

69.118

98.551

0.681
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.319

100

0.623
  pilT Neisseria gonorrhoeae MS11

62.029

100

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.812

0.391
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment