Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCV30_RS13305 Genome accession   NZ_AP025460
Coordinates   2985499..2986536 (-) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio atlanticus strain CECT 7223     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2980499..2991536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV30_RS13280 rsmE 2981191..2981922 (+) 732 WP_065679491.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV30_RS13285 gshB 2981932..2982882 (+) 951 WP_004735482.1 glutathione synthase -
  OCV30_RS13290 - 2982991..2983554 (+) 564 WP_065679492.1 YqgE/AlgH family protein -
  OCV30_RS13295 ruvX 2983615..2984037 (+) 423 WP_009847698.1 Holliday junction resolvase RuvX -
  OCV30_RS13300 pilU 2984375..2985484 (-) 1110 WP_041472921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV30_RS13305 pilT 2985499..2986536 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV30_RS13310 - 2986563..2987279 (+) 717 WP_065679493.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV30_RS13315 proC 2987343..2988161 (+) 819 WP_065679494.1 pyrroline-5-carboxylate reductase -
  OCV30_RS13320 - 2988254..2988811 (+) 558 WP_012604836.1 YggT family protein -
  OCV30_RS13325 yggU 2988841..2989131 (+) 291 WP_017101445.1 DUF167 family protein YggU -
  OCV30_RS13330 - 2989160..2989591 (+) 432 WP_017101446.1 DUF4426 domain-containing protein -
  OCV30_RS13335 - 2989640..2990239 (+) 600 WP_065679495.1 XTP/dITP diphosphatase -
  OCV30_RS13340 hemW 2990239..2991426 (+) 1188 WP_065679496.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=91659 OCV30_RS13305 WP_004735478.1 2985499..2986536(-) (pilT) [Vibrio atlanticus strain CECT 7223]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=91659 OCV30_RS13305 WP_004735478.1 2985499..2986536(-) (pilT) [Vibrio atlanticus strain CECT 7223]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGCCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAGTTCGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGC
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCTGTGTTCCGTACCATTCCAGTAGAGATTCCCACCCTTGA
ACAGCTAGGGGCTCCTGAGATATTTGAAAGAATATCTAATTATGAAAAAGGGCTAGTATTGGTTACTGGGCCGACTGGTT
CAGGTAAGTCGACAACTTTGGCTGCTATGGTAGATTACGTTAACCGTAACCATAATAAACATATATTGACGATTGAAGAC
CCGATCGAATTTGTTCATACCAACAACAAATGCCTAATCAACCAACGAGAAGTGCACCGCGATACTCACAGCTTTAAAGC
GGCGTTGCGCAGTGCGTTACGTGAAGATCCAGACGTAATCCTTGTCGGCGAACTTCGTGACCAAGAGACGATTAGCTTAG
CGCTAACCGCAGCAGAAACTGGCCACTTAGTTTTTGGTACCTTGCATACTAGCTCTGCGGCAAAAACCATAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTAAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATTAGAAACTTGATCC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGCGCGGCACACGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTGATGGCGCAAGGCTTAGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment