Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV30_RS13300 Genome accession   NZ_AP025460
Coordinates   2984375..2985484 (-) Length   369 a.a.
NCBI ID   WP_041472921.1    Uniprot ID   A0A1C3IVG6
Organism   Vibrio atlanticus strain CECT 7223     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2979375..2990484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV30_RS13270 - 2979702..2980202 (+) 501 WP_065679489.1 SprT family zinc-dependent metalloprotease -
  OCV30_RS13275 - 2980300..2981031 (+) 732 WP_065679490.1 endonuclease -
  OCV30_RS13280 rsmE 2981191..2981922 (+) 732 WP_065679491.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV30_RS13285 gshB 2981932..2982882 (+) 951 WP_004735482.1 glutathione synthase -
  OCV30_RS13290 - 2982991..2983554 (+) 564 WP_065679492.1 YqgE/AlgH family protein -
  OCV30_RS13295 ruvX 2983615..2984037 (+) 423 WP_009847698.1 Holliday junction resolvase RuvX -
  OCV30_RS13300 pilU 2984375..2985484 (-) 1110 WP_041472921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV30_RS13305 pilT 2985499..2986536 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  OCV30_RS13310 - 2986563..2987279 (+) 717 WP_065679493.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCV30_RS13315 proC 2987343..2988161 (+) 819 WP_065679494.1 pyrroline-5-carboxylate reductase -
  OCV30_RS13320 - 2988254..2988811 (+) 558 WP_012604836.1 YggT family protein -
  OCV30_RS13325 yggU 2988841..2989131 (+) 291 WP_017101445.1 DUF167 family protein YggU -
  OCV30_RS13330 - 2989160..2989591 (+) 432 WP_017101446.1 DUF4426 domain-containing protein -
  OCV30_RS13335 - 2989640..2990239 (+) 600 WP_065679495.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41454.32 Da        Isoelectric Point: 5.6712

>NTDB_id=91658 OCV30_RS13300 WP_041472921.1 2984375..2985484(-) (pilU) [Vibrio atlanticus strain CECT 7223]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQDRRDEYKQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVVADKISEDDAFHSADSANDLRLMLKTKRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=91658 OCV30_RS13300 WP_041472921.1 2984375..2985484(-) (pilU) [Vibrio atlanticus strain CECT 7223]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTCTCTCAAAAGGCGTCGGATCTTTACATCACAGTCGATGCGCCAATTCT
ATTTCGTGTGGATGGTGAACTACGACCTCAGGGAGAGAAGTTGAATGCTGATCAAGTATCTCAGTTACTTGATGCAATGA
TGGATCAAGATCGACGCGATGAATACAAGCAAACGCGTGAGGCTAATTTTGCCATCGTGCGTGATTTTGGTCGTTTTCGT
GTGAGTGCATTCTTTCAGCGAGAGCTACCTGGAGCGGTAATTCGTCGCATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGACGTACTACAAGACCTTTCAATCGCTAAGCGCGGGCTTGTGCTGGTGGTTGGTGCGACTGGCTCTGGTA
AATCGACCTCGATGGCTGCGATGACAGGCTATCGCAATACCAATCGTTCAGGTCATATCTTGACGGTTGAAGACCCGATT
GAATTCGTACACGAACATAAAAAGTGCATCGTGACTCAACGAGAGGTTGGACTCGACACCGAAAGCTATGAAGTCGCGCT
TAAAAACTCGTTACGCCAAGCCCCTGATATGATTTTGATTGGCGAAATCCGTAGTCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCAACCCTGCATGCCAATAACGCTAACCAAGCGCTAGAGCGTATTCTTCAC
TTGGTGCCGAAAGAGCAGAAAGAGCAGTTCTTGTTTGATCTGTCGATGAATCTGCGTGGTGTGGTCGCTCAGCAGTTAAT
ACGCGATAAAAATGGCAATGGTCGTCATGGTGTCTTCGAAATTCTTCTAAATAGCCCTCGTATATCTGACTTGATTCGTC
GTGGTGAGTTGCATGAGTTAAAAGCGACCATGGCCAAATCGAAAGAGATTGGTATGCAGACTTTTGACCAAGCTTTGTAT
GATTTAGTAGTTGCGGACAAGATTAGCGAGGATGATGCATTCCATAGTGCAGATTCTGCCAATGACTTGCGTTTAATGCT
TAAGACCAAACGTGGCGATGATGACTACGGAACTGGTGCGTTGGCTGGCGTTAAGATTGATATGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3IVG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.598

99.729

0.854
  pilU Pseudomonas stutzeri DSM 10701

55.795

100

0.561
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain ERS1305867

42.982

92.683

0.398
  pilT Legionella pneumophila strain Lp02

42.982

92.683

0.398
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

91.87

0.385
  pilT Pseudomonas aeruginosa PAK

41.493

90.786

0.377
  pilT Acinetobacter baylyi ADP1

42.638

88.347

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.597

90.786

0.369
  pilT Vibrio cholerae strain A1552

41.538

88.076

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.076

0.366

Multiple sequence alignment