Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U3649_RS16825 Genome accession   NZ_CP141262
Coordinates   3857048..3858085 (-) Length   345 a.a.
NCBI ID   WP_407352439.1    Uniprot ID   -
Organism   Luteimonas sp. R10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3852048..3863085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3649_RS16815 - 3855097..3855732 (-) 636 Protein_3319 DUF6531 domain-containing protein -
  U3649_RS16820 (U3649_16720) pilU 3855793..3856923 (-) 1131 WP_407352438.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U3649_RS16825 (U3649_16725) pilT 3857048..3858085 (-) 1038 WP_407352439.1 type IV pilus twitching motility protein PilT Machinery gene
  U3649_RS16830 (U3649_16730) - 3858236..3858946 (+) 711 WP_407352440.1 YggS family pyridoxal phosphate-dependent enzyme -
  U3649_RS16835 (U3649_16735) proC 3859134..3859988 (+) 855 WP_407352441.1 pyrroline-5-carboxylate reductase -
  U3649_RS16840 (U3649_16740) - 3860216..3860713 (+) 498 WP_407352442.1 DUF4426 domain-containing protein -
  U3649_RS16845 (U3649_16745) - 3860848..3861243 (-) 396 WP_407352443.1 DoxX family protein -
  U3649_RS16850 (U3649_16750) - 3861463..3862867 (+) 1405 Protein_3326 MFS transporter -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38364.08 Da        Isoelectric Point: 6.7268

>NTDB_id=916511 U3649_RS16825 WP_407352439.1 3857048..3858085(-) (pilT) [Luteimonas sp. R10]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTVPSEVLTLEDLGCPPVFRQLIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHVLSVED
PIEFVHTAQKCLINQREVHRDTHGFNEALRSALREDPDYILVGEMRDIETIRLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQSVGMMTLDQHL
QDLVKRGLVTRAQAKDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=916511 U3649_RS16825 WP_407352439.1 3857048..3858085(-) (pilT) [Luteimonas sp. R10]
ATGGATATCGCCGAACTGTTGGCGTTCTCGGTCAAGAACAAGGCATCGGACCTGCATCTGTCGGCGGGACTTCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGCCGCATCAACATCCCGGCGCTCGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATCCCGGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCGTGCCCTCGGAAGTGCTGACCCTGGA
GGACCTGGGCTGTCCGCCGGTGTTCCGGCAGCTGATCGACCAGCCGCAAGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAGTACGGCCACGTCCTCAGCGTCGAGGAC
CCGATCGAGTTCGTGCACACCGCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
GGCCCTGCGCTCGGCGCTGCGCGAGGATCCCGACTACATCCTGGTCGGCGAGATGCGCGACATCGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGCCACCCTGCATACCTCGAGCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATCAGCCAGGC
CCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAGATCATGGTCGGCACCCCCGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGTCGGTCGGCATGATGACTCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCGGCCTGGTCACGCGTGCGCAGGCCAAGGACTACGCGAAGGACAAACGCCTGTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Acinetobacter nosocomialis M2

77.907

99.71

0.777
  pilT Acinetobacter baumannii D1279779

77.907

99.71

0.777
  pilT Acinetobacter baumannii strain A118

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.581

98.261

0.762
  pilT Acinetobacter baylyi ADP1

76.453

99.71

0.762
  pilT Legionella pneumophila strain ERS1305867

71.802

99.71

0.716
  pilT Legionella pneumophila strain Lp02

71.802

99.71

0.716
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.605

95.362

0.664
  pilT Vibrio cholerae strain A1552

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

63.93

98.841

0.632
  pilT Neisseria gonorrhoeae MS11

63.636

98.841

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

100

0.487
  pilU Pseudomonas stutzeri DSM 10701

40.805

100

0.412
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388