Detailed information NTG0916510

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U3649_RS16820
• Genome accession: NZ_CP141262
• Coordinates: 3855793..3856923 (-)
• Length: 376 a.a.
• NCBI ID: WP_407352438.1
• Uniprot ID: -
• Organism: Luteimonas sp. R10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3850793..3861923

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U3649_RS16805 (U3649_16710)	-	3851076..3851414 (-)	339	WP_407352437.1	hypothetical protein	-
U3649_RS16810 (U3649_16715)	-	3851425..3854913 (-)	3489	WP_407354652.1	RHS repeat-associated core domain-containing protein	-
U3649_RS16815	-	3855097..3855732 (-)	636	Protein_3319	DUF6531 domain-containing protein	-
U3649_RS16820 (U3649_16720)	pilU	3855793..3856923 (-)	1131	WP_407352438.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
U3649_RS16825 (U3649_16725)	pilT	3857048..3858085 (-)	1038	WP_407352439.1	type IV pilus twitching motility protein PilT	Machinery gene
U3649_RS16830 (U3649_16730)	-	3858236..3858946 (+)	711	WP_407352440.1	YggS family pyridoxal phosphate-dependent enzyme	-
U3649_RS16835 (U3649_16735)	proC	3859134..3859988 (+)	855	WP_407352441.1	pyrroline-5-carboxylate reductase	-
U3649_RS16840 (U3649_16740)	-	3860216..3860713 (+)	498	WP_407352442.1	DUF4426 domain-containing protein	-
U3649_RS16845 (U3649_16745)	-	3860848..3861243 (-)	396	WP_407352443.1	DoxX family protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41802.03 Da
• Isoelectric Point: 6.6610

>NTDB_id=916510 U3649_RS16820 WP_407352438.1 3855793..3856923(-) (pilU) [Luteimonas sp. R10]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSIKVHGKISPITQNPLTPQQSRDLVLNVMSPPQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPIIKTLAMTKRGIIVFVGATGTGKSTSLASMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKARRVAMEIMLGTPLVQDYIRDGEIHKLKDVMKESTNLGMKTF
DQALFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLSQGLDGVEVAEAN

Nucleotide

• Length: 1131 bp

>NTDB_id=916510 U3649_RS16820 WP_407352438.1 3855793..3856923(-) (pilU) [Luteimonas sp. R10]
ATGAGCAGCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATCAAGGTCCACGGCAAGATCTCGCCGATCACCCAGAACCCGCTCACGCCGCAGCAGTCGCGCGACCTGG
TGCTCAACGTGATGTCGCCGCCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACCAGGATCCC
GACGATCGAGGAGCTCAACCTGCCGCCGATCATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCGTCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGTCGATGATCGGCTACCGCAACCAGAACTCCACCGGCCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGTATCGACACCGACAG
CTGGGAGAACGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCGCCTTTGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCGGCCAGCTGCTGATGGACCTCTCGCTCAACCTCAAGGGCGTCGT
CGCCCAGCAGCTGATCCCGACCCCGGACGGCAAGGCCCGCCGCGTCGCCATGGAGATCATGCTCGGCACTCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGACGTCATGAAGGAGTCCACCAACCTCGGCATGAAGACCTTC
GACCAGGCCCTGTTCGAGCTCTACCAGGCCGGCGAGATCTCCTACGAGGACGCCCTGCGCTACGCCGACTCCGCCAACGA
GGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCCGCACGCTCAGCCAGGGGCTGGACGGGGTGGAGGTCGCGG
AGGCGAACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	67.847	97.606	0.662
pilU	Acinetobacter baylyi ADP1	63.866	94.947	0.606
pilU	Vibrio cholerae strain A1552	53.652	94.681	0.508
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.109	90.691	0.391
pilT	Pseudomonas aeruginosa PAK	40.597	89.096	0.362