Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U5817_RS21650 Genome accession   NZ_CP141259
Coordinates   4628966..4630006 (+) Length   346 a.a.
NCBI ID   WP_169127430.1    Uniprot ID   -
Organism   Aromatoleum evansii strain MAY27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4623966..4635006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U5817_RS21625 (U5817_21565) - 4624928..4626349 (-) 1422 WP_407278778.1 pyridoxal phosphate-dependent decarboxylase family protein -
  U5817_RS21630 (U5817_21570) - 4626447..4626740 (-) 294 WP_169127424.1 DUF167 family protein -
  U5817_RS21635 (U5817_21575) - 4626743..4627321 (-) 579 WP_169127426.1 YggT family protein -
  U5817_RS21640 (U5817_21580) proC 4627326..4628138 (-) 813 WP_169127462.1 pyrroline-5-carboxylate reductase -
  U5817_RS21645 (U5817_21585) - 4628162..4628863 (-) 702 WP_407278779.1 YggS family pyridoxal phosphate-dependent enzyme -
  U5817_RS21650 (U5817_21590) pilT 4628966..4630006 (+) 1041 WP_169127430.1 type IV pilus twitching motility protein PilT Machinery gene
  U5817_RS21655 (U5817_21595) pilU 4630143..4631276 (+) 1134 WP_169127432.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U5817_RS21660 (U5817_21600) - 4631408..4632568 (-) 1161 WP_407278780.1 class I SAM-dependent RNA methyltransferase -
  U5817_RS21665 (U5817_21605) - 4632626..4633036 (-) 411 WP_169127436.1 CopD family protein -
  U5817_RS21670 (U5817_21610) - 4633046..4633930 (-) 885 WP_169127438.1 SDR family oxidoreductase -
  U5817_RS21675 (U5817_21615) - 4633952..4634971 (+) 1020 WP_407278782.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38555.29 Da        Isoelectric Point: 7.0157

>NTDB_id=916480 U5817_RS21650 WP_169127430.1 4628966..4630006(+) (pilT) [Aromatoleum evansii strain MAY27]
MDITELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPPMEHKDVHSMVYDIMNDQQRKQYEEVLECDFSFAVPNLAR
FRVNAFNQNRGAAAVFRTIPSKVLTLEELNCPKIFKDISNQPRGIVLVTGPTGSGKSTTLAAMVDHINDNEYGHILTIED
PIEFVHESKRCLINQREVARDTLSFNNALRSALREDPDVILVGEMRDLETIRLALTGAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNVGMQTLDQC
LLDLVRRNIVSPAEAKMKAQNKDNFG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=916480 U5817_RS21650 WP_169127430.1 4628966..4630006(+) (pilT) [Aromatoleum evansii strain MAY27]
ATGGACATCACCGAACTGCTGGCCTTTGCGGTCAAGAACAAGGCCTCCGACCTCCATCTCTCATCCGGCCTGCCGCCCAT
GATCCGCGTGCATGGCGACGTGCGGCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTGCACTCCATGGTGTACGACA
TCATGAACGACCAGCAGCGCAAGCAGTACGAGGAAGTGCTGGAGTGCGACTTCTCGTTCGCGGTGCCCAACCTCGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGTGGCGCGGCCGCCGTGTTCCGGACCATTCCGTCCAAGGTGCTGACGCTCGA
GGAGCTCAACTGCCCGAAGATCTTCAAGGACATCTCCAACCAGCCGCGTGGCATCGTGCTCGTCACCGGGCCGACCGGCT
CGGGCAAGTCGACGACGCTCGCGGCAATGGTCGACCACATCAACGACAACGAATACGGCCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTGCATGAGTCGAAGCGTTGCCTGATCAACCAGCGCGAAGTCGCGCGCGACACGCTGTCCTTCAACAA
CGCGCTGCGTTCGGCGCTACGCGAGGACCCGGACGTGATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTCG
CACTCACCGGCGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCGGCCGCCAAGACCATCGACCGTATC
GTCGACGTCTTCCCGGCCGCCGAGAAGGAGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCCGTCATCTCGCAGAC
CCTGCTGAAGACCAAGGACGGCCAGGGTCGCGTCGCCGCCCACGAGATCATGATCGGCACCCCCGCCATCCGCAACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTACTCGTCGATCCAGACCGGCCAGAACGTCGGCATGCAGACGCTGGACCAGTGC
CTGCTCGATCTCGTGCGCCGCAACATCGTGTCGCCCGCCGAAGCCAAGATGAAGGCGCAGAACAAGGACAACTTCGGCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.551

99.711

0.783
  pilT Acinetobacter baumannii D1279779

78.551

99.711

0.783
  pilT Acinetobacter baumannii strain A118

78.551

99.711

0.783
  pilT Pseudomonas stutzeri DSM 10701

77.391

99.711

0.772
  pilT Pseudomonas aeruginosa PAK

77.101

99.711

0.769
  pilT Acinetobacter baylyi ADP1

76.522

99.711

0.763
  pilT Legionella pneumophila strain Lp02

73.333

99.711

0.731
  pilT Legionella pneumophila strain ERS1305867

73.333

99.711

0.731
  pilT Neisseria meningitidis 8013

71.884

99.711

0.717
  pilT Neisseria gonorrhoeae MS11

71.594

99.711

0.714
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.941

98.266

0.668
  pilT Vibrio cholerae strain A1552

67.941

98.266

0.668
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.683

95.665

0.523
  pilU Acinetobacter baylyi ADP1

41.379

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.711

0.402
  pilU Vibrio cholerae strain A1552

40.29

99.711

0.402