Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U5817_RS21655 Genome accession   NZ_CP141259
Coordinates   4630143..4631276 (+) Length   377 a.a.
NCBI ID   WP_169127432.1    Uniprot ID   -
Organism   Aromatoleum evansii strain MAY27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4625143..4636276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U5817_RS21630 (U5817_21570) - 4626447..4626740 (-) 294 WP_169127424.1 DUF167 family protein -
  U5817_RS21635 (U5817_21575) - 4626743..4627321 (-) 579 WP_169127426.1 YggT family protein -
  U5817_RS21640 (U5817_21580) proC 4627326..4628138 (-) 813 WP_169127462.1 pyrroline-5-carboxylate reductase -
  U5817_RS21645 (U5817_21585) - 4628162..4628863 (-) 702 WP_407278779.1 YggS family pyridoxal phosphate-dependent enzyme -
  U5817_RS21650 (U5817_21590) pilT 4628966..4630006 (+) 1041 WP_169127430.1 type IV pilus twitching motility protein PilT Machinery gene
  U5817_RS21655 (U5817_21595) pilU 4630143..4631276 (+) 1134 WP_169127432.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U5817_RS21660 (U5817_21600) - 4631408..4632568 (-) 1161 WP_407278780.1 class I SAM-dependent RNA methyltransferase -
  U5817_RS21665 (U5817_21605) - 4632626..4633036 (-) 411 WP_169127436.1 CopD family protein -
  U5817_RS21670 (U5817_21610) - 4633046..4633930 (-) 885 WP_169127438.1 SDR family oxidoreductase -
  U5817_RS21675 (U5817_21615) - 4633952..4634971 (+) 1020 WP_407278782.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  U5817_RS21680 (U5817_21620) - 4634976..4636112 (+) 1137 WP_169127442.1 EAL and HDOD domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42175.55 Da        Isoelectric Point: 6.4206

>NTDB_id=916481 U5817_RS21655 WP_169127432.1 4630143..4631276(+) (pilU) [Aromatoleum evansii strain MAY27]
MERDQALKFMHDLLRLMVQKNGSDLFITAGFPPAIKVDGRVIPQSNQSLTQQHTAELARAVMNDRQAAEFESTKECNFAI
SPAGIGRFRANAFIQQGKVGLVLRTIAQKIPTFDDLGMPAVLRDVAMAKRGLVIFVGGTGTGKTTSLAAMVDFRNEHSYG
HIITVEDPIEYVHLHKNCIVTQREIGIDTDTWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQALDRIINFFPEDRRQQLLMDLSLNLRALISQRLLPKKGEKGRVPAVEVLLNSPLISDLIFKGEVPGIKEVMKRSRELG
MQTFDQALFDLYEEERITYEDALRNADSVNDLRLQIKLNSKLGEKDLVSGIQHLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=916481 U5817_RS21655 WP_169127432.1 4630143..4631276(+) (pilU) [Aromatoleum evansii strain MAY27]
ATGGAACGCGATCAGGCCCTCAAGTTCATGCACGACCTGCTCAGGCTGATGGTGCAGAAGAACGGCTCCGACCTCTTCAT
CACCGCCGGCTTCCCGCCCGCGATCAAGGTCGACGGACGCGTCATCCCGCAGTCCAACCAGTCGCTCACGCAGCAGCACA
CCGCCGAGCTCGCGCGCGCGGTCATGAACGACCGCCAGGCCGCGGAGTTCGAATCGACCAAGGAATGCAACTTCGCGATC
TCGCCCGCGGGCATCGGCCGTTTCCGCGCCAACGCCTTCATCCAGCAGGGCAAGGTCGGCCTCGTGCTGCGCACGATCGC
GCAGAAGATCCCGACCTTCGACGATCTGGGCATGCCCGCGGTGCTGCGCGACGTCGCGATGGCCAAGCGCGGTCTCGTGA
TCTTCGTCGGCGGCACCGGTACCGGCAAGACGACCTCGCTCGCCGCGATGGTGGACTTCCGCAACGAGCACTCCTACGGC
CACATCATCACCGTCGAGGACCCGATCGAATACGTGCATCTGCACAAGAACTGCATCGTCACGCAGCGCGAGATCGGCAT
CGACACCGACACCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCCCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACGATGGACTACGCGATCGCTTTCGCCGAGACCGGCCACCTGTGCCTCGCCACGCTGCACGCGAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAGCTGCTGATGGACCTCTCGCTCAACCT
GCGCGCGCTGATCTCGCAGCGCCTGCTGCCGAAGAAGGGCGAGAAGGGACGCGTGCCGGCCGTCGAGGTGCTGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGCCCGGCATCAAGGAAGTGATGAAGCGCTCGCGCGAACTCGGG
ATGCAGACCTTCGACCAGGCGCTCTTCGACCTCTACGAGGAGGAGCGGATCACCTACGAGGACGCCCTGCGCAACGCCGA
CTCGGTGAACGACTTGCGCCTGCAGATCAAGCTCAACAGCAAGCTCGGCGAGAAGGATCTCGTATCAGGGATCCAGCACC
TCGACATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.912

96.552

0.607
  pilU Acinetobacter baylyi ADP1

59.49

93.634

0.557
  pilU Vibrio cholerae strain A1552

56.012

90.451

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.859

0.387
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.859

0.371
  pilT Pseudomonas aeruginosa PAK

41.791

88.859

0.371
  pilT Legionella pneumophila strain ERS1305867

41.692

87.798

0.366
  pilT Legionella pneumophila strain Lp02

41.692

87.798

0.366
  pilT Acinetobacter nosocomialis M2

41.692

87.798

0.366
  pilT Acinetobacter baylyi ADP1

42.812

84.881

0.363