Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU36_RS11705 Genome accession   NZ_AP025458
Coordinates   2617511..2618548 (-) Length   345 a.a.
NCBI ID   WP_261838151.1    Uniprot ID   -
Organism   Vibrio artabrorum strain CECT 7226     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2612511..2623548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU36_RS11675 rsmE 2613207..2613938 (+) 732 WP_261838145.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU36_RS11680 gshB 2613948..2614898 (+) 951 WP_261838146.1 glutathione synthase -
  OCU36_RS11685 - 2615015..2615578 (+) 564 WP_261838147.1 YqgE/AlgH family protein -
  OCU36_RS11690 ruvX 2615639..2616061 (+) 423 WP_261838148.1 Holliday junction resolvase RuvX -
  OCU36_RS11695 - 2616160..2616321 (+) 162 WP_261838149.1 hypothetical protein -
  OCU36_RS11700 pilU 2616387..2617496 (-) 1110 WP_261838150.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU36_RS11705 pilT 2617511..2618548 (-) 1038 WP_261838151.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU36_RS11710 - 2618575..2619279 (+) 705 WP_261838152.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU36_RS11715 proC 2619361..2620179 (+) 819 WP_261838153.1 pyrroline-5-carboxylate reductase -
  OCU36_RS11720 - 2620255..2620812 (+) 558 WP_261838154.1 YggT family protein -
  OCU36_RS11725 yggU 2620842..2621132 (+) 291 WP_009847703.1 DUF167 family protein YggU -
  OCU36_RS11730 - 2621161..2621592 (+) 432 WP_261838155.1 DUF4426 domain-containing protein -
  OCU36_RS11735 - 2621639..2622238 (+) 600 WP_261838156.1 XTP/dITP diphosphatase -
  OCU36_RS11740 hemW 2622238..2623425 (+) 1188 WP_261838157.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38327.96 Da        Isoelectric Point: 6.2291

>NTDB_id=91614 OCU36_RS11705 WP_261838151.1 2617511..2618548(-) (pilT) [Vibrio artabrorum strain CECT 7226]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFDIMDDLQRGEFEEKLEVDFSFELPNVGR
FRVNAFHQSRGCSAVFRTIPVEIPTLEQLGTPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRHHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDPEEVEKKIEIESSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=91614 OCU36_RS11705 WP_261838151.1 2617511..2618548(-) (pilT) [Vibrio artabrorum strain CECT 7226]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCAGATGTGCATCGTTTGGTGTTTGATA
TTATGGACGATCTACAGCGTGGTGAGTTTGAAGAGAAGCTGGAAGTCGACTTCTCTTTTGAATTGCCCAACGTTGGTCGT
TTTCGTGTGAATGCTTTTCATCAATCTCGTGGTTGCTCGGCTGTTTTTCGAACCATCCCAGTGGAAATTCCAACTTTAGA
GCAGTTAGGCACACCTGAAATATTTGAAAAAATTGCTAACTACGAAAAAGGCTTAGTGCTCGTCACTGGGCCAACAGGTT
CGGGTAAATCAACAACACTGGCAGCGATGGTCGATTATGTAAACCGTCATCATAATAAGCACATATTAACGATCGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAATGTCTCATTAACCAACGAGAAGTACACCGTGATACACACAGCTTTAAAGC
GGCGTTGCGCAGTGCTCTGCGTGAAGATCCTGATGTGATCCTTGTGGGGGAGCTTCGTGATCAAGAAACCATTAGCTTAG
CGCTAACCGCTGCTGAAACAGGGCATTTAGTTTTCGGAACTCTACACACCAGTTCTGCGGCAAAAACCATAGATCGTATT
ATCGATGTATTTCCGGGTAGCGATAAAGGTATGGTTCGTTCCATGCTGTCTGAATCATTGCGCTCAGTGATTGCTCAGAA
GCTGTTAAAGCGTGTCGGTGGTGGCCGTGTAGCTTGCCATGAAATCATGATGGCGACACCTGCGATCAGAAACTTGATCC
GTGAAGATAAAGTTGCACAGATGTACTCGATCATTCAAACGGGCGCAGCTCATGGTATGCAAACCATGGAGCAAAATGCG
AAACAACTGATGGCGCAAGGCTTGGTTGATCCAGAGGAAGTCGAGAAAAAGATCGAAATTGAATCCTCCATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

72.289

96.232

0.696
  pilT Acinetobacter baumannii strain A118

72.289

96.232

0.696
  pilT Acinetobacter nosocomialis M2

71.988

96.232

0.693
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Legionella pneumophila strain Lp02

68.085

95.362

0.649
  pilT Legionella pneumophila strain ERS1305867

68.085

95.362

0.649
  pilT Neisseria meningitidis 8013

62.899

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.609

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

92.174

0.487
  pilU Vibrio cholerae strain A1552

43.692

94.203

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.812

0.391
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374

Multiple sequence alignment