Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU36_RS11700 Genome accession   NZ_AP025458
Coordinates   2616387..2617496 (-) Length   369 a.a.
NCBI ID   WP_261838150.1    Uniprot ID   -
Organism   Vibrio artabrorum strain CECT 7226     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2611387..2622496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU36_RS11665 - 2611726..2612229 (+) 504 WP_261838143.1 SprT family zinc-dependent metalloprotease -
  OCU36_RS11670 - 2612323..2613054 (+) 732 WP_261838144.1 endonuclease -
  OCU36_RS11675 rsmE 2613207..2613938 (+) 732 WP_261838145.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU36_RS11680 gshB 2613948..2614898 (+) 951 WP_261838146.1 glutathione synthase -
  OCU36_RS11685 - 2615015..2615578 (+) 564 WP_261838147.1 YqgE/AlgH family protein -
  OCU36_RS11690 ruvX 2615639..2616061 (+) 423 WP_261838148.1 Holliday junction resolvase RuvX -
  OCU36_RS11695 - 2616160..2616321 (+) 162 WP_261838149.1 hypothetical protein -
  OCU36_RS11700 pilU 2616387..2617496 (-) 1110 WP_261838150.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU36_RS11705 pilT 2617511..2618548 (-) 1038 WP_261838151.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU36_RS11710 - 2618575..2619279 (+) 705 WP_261838152.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU36_RS11715 proC 2619361..2620179 (+) 819 WP_261838153.1 pyrroline-5-carboxylate reductase -
  OCU36_RS11720 - 2620255..2620812 (+) 558 WP_261838154.1 YggT family protein -
  OCU36_RS11725 yggU 2620842..2621132 (+) 291 WP_009847703.1 DUF167 family protein YggU -
  OCU36_RS11730 - 2621161..2621592 (+) 432 WP_261838155.1 DUF4426 domain-containing protein -
  OCU36_RS11735 - 2621639..2622238 (+) 600 WP_261838156.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41555.71 Da        Isoelectric Point: 6.4356

>NTDB_id=91613 OCU36_RS11700 WP_261838150.1 2616387..2617496(-) (pilU) [Vibrio artabrorum strain CECT 7226]
MELNQILEGMLSQKASDLYITVDAPLLFRVDGELRPQGEKLNVTQVTRLLDMMMDQEKRHEYKKTREANYAIVRDFGRFR
VSAFFQRELPGAVIRRIETDIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNANRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTDSYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCIATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGDLHELKATMAKSKEIGMQTFDQALY
HLVVAGKISEEDAFHGADSANDLRLMLKTRCGDIDYGTGPLADVKIDMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=91613 OCU36_RS11700 WP_261838150.1 2616387..2617496(-) (pilU) [Vibrio artabrorum strain CECT 7226]
ATGGAATTGAATCAAATCCTTGAGGGAATGCTGTCTCAAAAAGCGTCGGATCTTTATATCACCGTTGATGCACCGCTCCT
GTTTCGTGTAGATGGTGAATTACGGCCTCAAGGAGAGAAGCTGAATGTCACTCAGGTTACTCGGCTACTTGATATGATGA
TGGATCAAGAGAAGCGTCATGAATATAAAAAAACGCGTGAGGCGAACTATGCCATTGTGCGGGACTTTGGTCGCTTTCGT
GTTAGTGCATTCTTTCAACGAGAGCTTCCAGGCGCGGTTATTCGACGCATCGAGACCGATATCCCAACGTTTGAACAGTT
AAAGCTTCCAGATGTACTACAAGATCTTTCTATTGCGAAGCGTGGACTGGTGCTGGTGGTTGGGGCAACAGGTTCTGGTA
AATCAACCTCAATGGCTGCGATGACAGGTTATCGTAACGCTAACCGCTCAGGGCACATATTGACCGTTGAAGACCCAATT
GAGTTCGTCCACGAGCACAAGAAGTGCATTGTGACTCAGCGTGAGGTCGGGCTCGACACTGATAGTTATGAAATTGCGCT
TAAGAATTCATTACGCCAAGCACCAGATATGATCTTGATTGGCGAAATCCGTAGCCGCGAAACGATGGAGTATGCGATGA
CCTTTGCTGAAACGGGTCACTTGTGTATTGCAACGCTGCACGCCAATAATGCCAACCAAGCGTTAGAACGTATTCTTCAC
TTGGTGCCGAAAGAGCAAAAAGAGCAGTTCTTGTTTGATCTGTCGATGAATTTACGTGGTGTCGTGGCTCAGCAACTCAT
TCGCGATAAGAATGGCAGCGGGCGCCATGGTGTATTTGAAATTCTGTTGAATAGCCCACGAGTGTCTGACTTGATTCGTC
GTGGCGATCTCCATGAGCTAAAAGCAACCATGGCGAAATCGAAGGAAATTGGTATGCAAACCTTTGATCAAGCTTTGTAT
CATTTAGTGGTTGCAGGCAAGATCAGCGAAGAAGATGCATTTCATGGTGCCGATTCTGCTAATGACTTACGCTTAATGCT
AAAAACTCGATGTGGTGATATAGATTATGGAACGGGACCATTGGCAGACGTTAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.114

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

58.286

94.851

0.553
  pilU Acinetobacter baylyi ADP1

53.561

95.122

0.509
  pilT Legionella pneumophila strain Lp02

42.398

92.683

0.393
  pilT Legionella pneumophila strain ERS1305867

42.398

92.683

0.393
  pilT Acinetobacter baumannii D1279779

41.493

90.786

0.377
  pilT Acinetobacter baumannii strain A118

41.493

90.786

0.377
  pilT Acinetobacter nosocomialis M2

41.493

90.786

0.377
  pilT Pseudomonas aeruginosa PAK

41.493

90.786

0.377
  pilT Vibrio cholerae strain A1552

40.719

90.515

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.515

0.369
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.975

87.805

0.369
  pilT Pseudomonas stutzeri DSM 10701

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

40.785

89.702

0.366

Multiple sequence alignment