Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U3H55_RS03070 Genome accession   NZ_CP140792
Coordinates   640899..641933 (+) Length   344 a.a.
NCBI ID   WP_042063177.1    Uniprot ID   A0A6S5C6A2
Organism   Aeromonas allosaccharophila strain M01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 635899..646933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3H55_RS03040 - 636563..637759 (+) 1197 WP_322863374.1 NupC/NupG family nucleoside CNT transporter -
  U3H55_RS03045 - 637863..638282 (-) 420 WP_033135536.1 DUF4426 domain-containing protein -
  U3H55_RS03050 yggU 638326..638628 (-) 303 WP_005341417.1 DUF167 family protein YggU -
  U3H55_RS03055 - 638628..639179 (-) 552 WP_042011910.1 YggT family protein -
  U3H55_RS03060 proC 639191..640015 (-) 825 WP_322863375.1 pyrroline-5-carboxylate reductase -
  U3H55_RS03065 - 640158..640859 (-) 702 WP_322863376.1 YggS family pyridoxal phosphate-dependent enzyme -
  U3H55_RS03070 pilT 640899..641933 (+) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  U3H55_RS03075 pilU 641962..643071 (+) 1110 WP_322864596.1 type IVa pilus ATPase TapU Machinery gene
  U3H55_RS03080 yaaA 643119..643892 (+) 774 WP_322863377.1 peroxide stress protein YaaA -
  U3H55_RS03085 - 644087..644512 (+) 426 WP_322863378.1 hypothetical protein -
  U3H55_RS03090 - 644874..645365 (-) 492 WP_322863379.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  U3H55_RS03095 arfB 645398..645814 (-) 417 WP_075113904.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  U3H55_RS03100 glnK 646016..646354 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38291.00 Da        Isoelectric Point: 6.7439

>NTDB_id=915595 U3H55_RS03070 WP_042063177.1 640899..641933(+) (pilT) [Aeromonas allosaccharophila strain M01]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKISEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=915595 U3H55_RS03070 WP_042063177.1 640899..641933(+) (pilT) [Aeromonas allosaccharophila strain M01]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTATGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTTAACGCCTTCCAGCAGGCGCGCGGCTCCGGCGCGGTATTTCGTACCATCCCCAGCACGGTCTTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTTCGCAAGATCTCCGAATACCCGCGCGGTCTGGTGCTGGTGACCGGCCCGACCGGTT
CCGGTAAATCAACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATTCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGAACAAGCGTTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCTCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGTGACCTCGAGACCATTCGTCTGG
CCATGACGGCCGCCGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGTGCGGAGAAGGACATGGTACGTTCCATGCTTTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGTATTGGTGGCGGCCGGGTGGCGGCACACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTGATCC
GTGAGGACAAGATCGCCCAGCTTTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5C6A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.843

99.709

0.776
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter baumannii D1279779

77.551

99.709

0.773
  pilT Acinetobacter baumannii strain A118

77.551

99.709

0.773
  pilT Pseudomonas stutzeri DSM 10701

76.385

99.709

0.762
  pilT Pseudomonas aeruginosa PAK

75.802

99.709

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.353

98.837

0.715
  pilT Legionella pneumophila strain ERS1305867

72.353

98.837

0.715
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404