Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U3H55_RS03075 Genome accession   NZ_CP140792
Coordinates   641962..643071 (+) Length   369 a.a.
NCBI ID   WP_322864596.1    Uniprot ID   -
Organism   Aeromonas allosaccharophila strain M01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636962..648071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3H55_RS03045 - 637863..638282 (-) 420 WP_033135536.1 DUF4426 domain-containing protein -
  U3H55_RS03050 yggU 638326..638628 (-) 303 WP_005341417.1 DUF167 family protein YggU -
  U3H55_RS03055 - 638628..639179 (-) 552 WP_042011910.1 YggT family protein -
  U3H55_RS03060 proC 639191..640015 (-) 825 WP_322863375.1 pyrroline-5-carboxylate reductase -
  U3H55_RS03065 - 640158..640859 (-) 702 WP_322863376.1 YggS family pyridoxal phosphate-dependent enzyme -
  U3H55_RS03070 pilT 640899..641933 (+) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  U3H55_RS03075 pilU 641962..643071 (+) 1110 WP_322864596.1 type IVa pilus ATPase TapU Machinery gene
  U3H55_RS03080 yaaA 643119..643892 (+) 774 WP_322863377.1 peroxide stress protein YaaA -
  U3H55_RS03085 - 644087..644512 (+) 426 WP_322863378.1 hypothetical protein -
  U3H55_RS03090 - 644874..645365 (-) 492 WP_322863379.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  U3H55_RS03095 arfB 645398..645814 (-) 417 WP_075113904.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  U3H55_RS03100 glnK 646016..646354 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  U3H55_RS03105 - 646613..647620 (+) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40966.84 Da        Isoelectric Point: 6.0885

>NTDB_id=915596 U3H55_RS03075 WP_322864596.1 641962..643071(+) (pilU) [Aeromonas allosaccharophila strain M01]
MNLDELLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVIRRIETRIPTFEDLQLPNILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSTDTMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLQIKLSGQDALGSGTLENVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=915596 U3H55_RS03075 WP_322864596.1 641962..643071(+) (pilU) [Aeromonas allosaccharophila strain M01]
ATGAATCTGGATGAATTGCTGAGCGAACTGGTCGAACGAAAGGGCTCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
TCTCAAGGTCAATGGCCATCTGGTGTCGCTGGGGGGCGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGAGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCCATCTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGATACGTCGCATTGAGACCCGCATCCCCACCTTTGA
AGATCTGCAGTTGCCCAACATCCTGCAGGAGGTCGCCATGGCCAAGCGGGGGTTGGTGCTGTTTGTCGGGGCCACCGGGG
CGGGTAAGTCTACCACTCAGGCGGCGATGATTGGCTATCGCAACCAGCATGCCGATGGCCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGCCGCAGTCTGGTGACCCAGCGTGAAGTGGGGATCGATACCGAGTCGTTCGATGT
GGCGCTGAAGAGCTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGAAGTACAGATACCATGGAGTTTG
CCCTGCAGTTCGCCGAAACCGGCCACCTTTGCCTCGCCACTCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGGATC
CTCCATCTGGTGCCGCAAGACAAACATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGAAAGCCATCGTAGCCCAGCA
ACTGGTGCCAAGCATGGATGGCAAGCGGCGTTTTGCCGCGTTCGAGGTGCTGCTCAATACCCCGCTGATCACCGACATCA
TCCGCAAGGGTGAGATGCACCGCCTCAAGGAGGTGATGACCAAATCCACCGAACTGGGCATGCAGACCTTCGATCAGGCG
CTGTTCAGCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCTCATGCCGACTCGGCTAATGATTTGCGTCT
GCAAATCAAGCTATCCGGTCAGGATGCGCTTGGCTCCGGCACGCTGGAGAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.141

99.729

0.61
  pilU Pseudomonas stutzeri DSM 10701

59.887

95.935

0.575
  pilU Acinetobacter baylyi ADP1

57.507

95.664

0.55
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377