Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U6038_RS03365 Genome accession   NZ_CP140715
Coordinates   698125..699162 (-) Length   345 a.a.
NCBI ID   WP_004694862.1    Uniprot ID   N9CT59
Organism   Acinetobacter johnsonii strain FAHZZU3110hy     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 681852..743772 698125..699162 within 0


Gene organization within MGE regions


Location: 681852..743772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6038_RS03275 (U6038_03275) - 682109..682396 (-) 288 WP_004694907.1 PA4642 family protein -
  U6038_RS03280 (U6038_03280) minE 682518..682790 (-) 273 WP_004694905.1 cell division topological specificity factor MinE -
  U6038_RS03285 (U6038_03285) minD 682793..683605 (-) 813 WP_010326008.1 septum site-determining protein MinD -
  U6038_RS03290 (U6038_03290) minC 683675..684406 (-) 732 WP_125281218.1 septum site-determining protein MinC -
  U6038_RS03295 (U6038_03295) - 684530..685579 (-) 1050 WP_374758889.1 preprotein translocase subunit SecA -
  U6038_RS03300 (U6038_03300) - 685590..686522 (-) 933 WP_279679100.1 acyltransferase -
  U6038_RS03305 (U6038_03305) - 686779..687429 (+) 651 WP_010326012.1 OmpA family protein -
  U6038_RS03310 (U6038_03310) - 687556..689595 (+) 2040 WP_004694892.1 UvrD-helicase domain-containing protein -
  U6038_RS03315 (U6038_03315) dut 689639..690091 (+) 453 WP_004694889.1 dUTP diphosphatase -
  U6038_RS03320 (U6038_03320) - 690288..691715 (+) 1428 WP_333655310.1 phosphomannomutase/phosphoglucomutase -
  U6038_RS03325 (U6038_03325) argB 691726..692628 (+) 903 WP_058869034.1 acetylglutamate kinase -
  U6038_RS03330 (U6038_03330) - 692797..693645 (+) 849 WP_333655313.1 class II glutamine amidotransferase -
  U6038_RS03335 (U6038_03335) - 693920..694375 (+) 456 WP_005400160.1 bacteriohemerythrin -
  U6038_RS03340 (U6038_03340) - 694518..695600 (+) 1083 WP_058869036.1 hypothetical protein -
  U6038_RS03345 (U6038_03345) - 695600..695923 (+) 324 WP_010326018.1 RnfH family protein -
  U6038_RS03350 (U6038_03350) - 695970..696368 (-) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  U6038_RS03355 (U6038_03355) fur 696481..696918 (+) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  U6038_RS03360 (U6038_03360) pilU 696977..698098 (-) 1122 WP_333655316.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U6038_RS03365 (U6038_03365) pilT 698125..699162 (-) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  U6038_RS03370 (U6038_03370) - 699285..699974 (+) 690 WP_125281224.1 YggS family pyridoxal phosphate-dependent enzyme -
  U6038_RS03375 (U6038_03375) - 700165..700899 (+) 735 WP_148826638.1 hypothetical protein -
  U6038_RS03380 (U6038_03380) - 701016..704612 (-) 3597 WP_333655320.1 AAA family ATPase -
  U6038_RS03385 (U6038_03385) - 704622..705875 (-) 1254 WP_333655323.1 exonuclease SbcCD subunit D -
  U6038_RS03390 (U6038_03390) - 706273..707250 (+) 978 WP_333655325.1 sensor domain-containing diguanylate cyclase -
  U6038_RS03395 (U6038_03395) - 707585..708037 (+) 453 WP_125281229.1 ABZJ_00895 family protein -
  U6038_RS03400 (U6038_03400) - 708091..708990 (+) 900 WP_205667061.1 TIGR01777 family oxidoreductase -
  U6038_RS03405 (U6038_03405) thiO 709019..710128 (-) 1110 WP_333655329.1 glycine oxidase ThiO -
  U6038_RS03410 (U6038_03410) hemB 710156..711169 (-) 1014 WP_058869046.1 porphobilinogen synthase -
  U6038_RS03415 (U6038_03415) - 711278..711775 (+) 498 WP_004694841.1 thioesterase family protein -
  U6038_RS03420 (U6038_03420) - 712023..713174 (+) 1152 WP_279687755.1 efflux RND transporter periplasmic adaptor subunit -
  U6038_RS03425 (U6038_03425) - 713181..714710 (+) 1530 WP_058869049.1 DHA2 family efflux MFS transporter permease subunit -
  U6038_RS03430 (U6038_03430) glpK 714807..716291 (-) 1485 WP_333655335.1 glycerol kinase GlpK -
  U6038_RS03440 (U6038_03440) - 716963..718099 (+) 1137 WP_333655337.1 tyrosine-type recombinase/integrase -
  U6038_RS03445 (U6038_03445) - 718226..719038 (-) 813 WP_333655340.1 hypothetical protein -
  U6038_RS03450 (U6038_03450) - 719293..721644 (+) 2352 WP_333655343.1 hypothetical protein -
  U6038_RS03455 (U6038_03455) - 722175..723101 (+) 927 WP_333655346.1 YagK/YfjJ domain-containing protein -
  U6038_RS03460 (U6038_03460) - 723185..724138 (+) 954 WP_333655349.1 hypothetical protein -
  U6038_RS03465 (U6038_03465) - 724288..724515 (+) 228 WP_333655352.1 helix-turn-helix transcriptional regulator -
  U6038_RS03470 (U6038_03470) - 724548..724766 (-) 219 WP_333655355.1 hypothetical protein -
  U6038_RS03475 (U6038_03475) - 725456..726664 (+) 1209 WP_333655358.1 hypothetical protein -
  U6038_RS03480 (U6038_03480) - 726815..727186 (+) 372 WP_333655362.1 hypothetical protein -
  U6038_RS03485 (U6038_03485) - 727339..727659 (+) 321 WP_184117949.1 hypothetical protein -
  U6038_RS03490 (U6038_03490) - 727724..728770 (+) 1047 WP_333655366.1 DUF932 domain-containing protein -
  U6038_RS03495 (U6038_03495) - 728914..729957 (+) 1044 WP_178905318.1 YqaJ viral recombinase family protein -
  U6038_RS03500 (U6038_03500) - 730074..731006 (+) 933 WP_279681078.1 hydrolase or metal-binding protein -
  U6038_RS03505 (U6038_03505) - 731074..731304 (+) 231 WP_016167128.1 helix-turn-helix transcriptional regulator -
  U6038_RS03510 (U6038_03510) - 731335..731559 (+) 225 Protein_667 hypothetical protein -
  U6038_RS03515 (U6038_03515) - 731640..734477 (+) 2838 WP_279681077.1 SNF2-related protein -
  U6038_RS03520 (U6038_03520) - 734520..734792 (+) 273 WP_106439006.1 hypothetical protein -
  U6038_RS03525 (U6038_03525) - 734851..736980 (-) 2130 WP_333655378.1 nuclease-related domain-containing DEAD/DEAH box helicase -
  U6038_RS03530 (U6038_03530) - 737173..740301 (+) 3129 WP_333655380.1 site-specific DNA-methyltransferase -
  U6038_RS03535 (U6038_03535) - 740308..743730 (+) 3423 WP_333655382.1 DEAD/DEAH box helicase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38292.96 Da        Isoelectric Point: 6.6658

>NTDB_id=915117 U6038_RS03365 WP_004694862.1 698125..699162(-) (pilT) [Acinetobacter johnsonii strain FAHZZU3110hy]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPSIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQTFKELCELPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVTKGVISPQTARTAAKQPETFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=915117 U6038_RS03365 WP_004694862.1 698125..699162(-) (pilT) [Acinetobacter johnsonii strain FAHZZU3110hy]
ATGGATATCACAGAATTACTAGCCTTTTCGGCTAAGAACGGGGCATCAGATTTACACTTATCTGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATCAATTTACCAGCTTTAGAACACAAAGAAGTGCATAAACTGGTCTATGACA
TCATGAATGATAAACAACGTCGTGATTTCGAAGAAAAACTAGAAACAGATTTTTCCTTTGAAGTTCCAAGCATTGCTCGT
TTTCGTGTGAACGCATTCAACCAAAATCGCGGTGCAGGTGCAGTATTCCGTACCATTCCGTCTAAAGTCCTGACCATGGA
AGACTTAGGTTTAGGACAAACCTTTAAAGAGTTGTGTGAATTGCCACGTGGTTTGGTTTTGGTGACAGGACCCACCGGTT
CTGGTAAATCTACCACTTTGGCGGCAATGATCGATTATATTAACGATAACCGCTATGACCACATCTTAACAGTTGAAGAC
CCTATCGAATTTGTACACCAGTCAAAAAAATGTTTGATCAACCAACGTGAAGTGCACCGTGATACACATGGTTTTAACGA
AGCACTGCGTTCTGCACTCCGTGAAGACCCCGATATTATCCTCGTGGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGCTCACTGCCGCTGAAACGGGTCACTTGGTCTTCGGTACACTACATACCACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGTTATCTGAATCGCTACAAGCCGTGATTTCTCAAAC
CTTACTCAAGAAAAATGGCGGTGGCCGTGTTGCCGCGCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAACAAAGTGGCACAGATGTATTCTGCTATTCAGACTGGTGCAAACTACGGTATGACCACACTGGATCAAAGTTTG
AAAAACTTGGTCACCAAAGGGGTGATCAGTCCACAAACTGCCCGTACAGCTGCCAAGCAACCTGAAACCTTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9CT59

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.452

97.391

0.667
  pilT Vibrio cholerae strain A1552

68.452

97.391

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

42.183

98.261

0.414
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

39.942

99.42

0.397