Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RI032_RS04110 Genome accession   NZ_CP140571
Coordinates   864348..865385 (-) Length   345 a.a.
NCBI ID   WP_006422551.1    Uniprot ID   -
Organism   Stenotrophomonas geniculata strain FLMAT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 859348..870385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI032_RS04085 - 859937..860644 (-) 708 WP_029379885.1 YitT family protein -
  RI032_RS04090 - 860755..860967 (-) 213 WP_049410257.1 DUF4287 domain-containing protein -
  RI032_RS04095 - 861061..861957 (+) 897 WP_043398312.1 LysR family transcriptional regulator -
  RI032_RS04100 - 862000..862983 (-) 984 WP_164191942.1 aldo/keto reductase -
  RI032_RS04105 pilU 863106..864200 (-) 1095 WP_049410297.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RI032_RS04110 pilT 864348..865385 (-) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  RI032_RS04115 - 865473..866150 (+) 678 WP_228355123.1 YggS family pyridoxal phosphate-dependent enzyme -
  RI032_RS04120 proC 866169..866990 (+) 822 WP_322547899.1 pyrroline-5-carboxylate reductase -
  RI032_RS04125 - 867050..867925 (-) 876 WP_057503301.1 LysR family transcriptional regulator -
  RI032_RS04130 - 868024..868626 (+) 603 WP_010483701.1 short chain dehydrogenase -
  RI032_RS04135 soxR 868700..869134 (-) 435 WP_019336938.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38644.38 Da        Isoelectric Point: 6.6652

>NTDB_id=914736 RI032_RS04110 WP_006422551.1 864348..865385(-) (pilT) [Stenotrophomonas geniculata strain FLMAT1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=914736 RI032_RS04110 WP_006422551.1 864348..865385(-) (pilT) [Stenotrophomonas geniculata strain FLMAT1]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGGATCAACATCCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCGTCGCTGGCGCGC
TTCCGTGTGAACGCGTTCAACCAGAACCGCGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTTGA
GGACCTGGCCTGCCCGCCGTTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCATGGTTTCAACGA
AGCACTGCGCTCGGCACTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAACTTCGTGACCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGCTCAGCGGCCAAGACCATCGATCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCCGTGATCTCGCAGGC
GCTGCTGAAGAAGGTTGGTGGCGGTCGTACCGCTGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGACTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391